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  • Coming to Grips with Biological Information Through Flexible Organic Electronics: Developing Bendable and Stretchable Biosensors and Device

    What do you associate with the word sensor? Perhaps technologies delivering automation in factories and other production sites? That may be what comes to mind, but advances in organic electronics are now driving the rapid development of biological sensors that measure physiological signals when in contact with the skin, organs, and other parts of the body.
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  • How Digital Microscopy can Contribute to Efficient Workflows for Microelectronics and Electronics

    This report explains how users can benefit from the digital microscope portfolio of Leica Microsystems to attain cost-effectiveness over entire workflows in research and development (R&D), product innovation, process engineering, production, quality control and assurance (QC/QA), and failure analysis (FA).
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  • Webinar: STED Nanoscopy in Combination with Optical Clearing Reveals Localization of Slit Diaphragm Proteins in the Kidney

    In this webinar, we show that optical clearing drastically increases the signal-to-noise ratio and staining quality, thus enabling STED nanoscopy of the subtlest elements of the kidney. In this way we show that optical clearing is not only a sample preparation technique to consider when imaging large mm-scale samples, but could also be fruitful when imaging at the nanoscale. Furthermore, the increased transparency of the optically cleared sample enables volumetric 3D STED imaging at sub-diffraction-limited resolution.
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  • Webinar: Introduction to Correlative Light and Electron Microscopy (CLEM)

    The webinar will provide an overview of the latest advances in Cryo CLEM, which acts as a powerful interface by combining the best of the light and electron microscopy worlds to overcome their independent barriers and determine the location of fluorescent labelled structures within the landscape of an electron micrograph and showcase how Cryo CLEM adds additional value to quantitative 3D imaging and tomography.
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  • Light Sheet Microscopy Turned Vertically

    Living cells and organisms often suffer from the high light intensities used for fluorescent imaging. Light sheet microscopy reduces phototoxic effects and bleaching by illuminating a specimen in only a single plane at a time. A new light sheet microscope combines light sheet and confocal microscopy in one system without compromising either functionality and allows the combination of the two methods, e.g. confocal photomanipulation with subsequent light sheet acquisition, for new applications.
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  • Using Telecentric Optical Systems to Optimize Forensic Image Accuracy and Reproducibility

    When the first compound microscopes were invented in 1590, scientists marveled at their new ability to see tiny objects and features that were previously invisible to the eye and therefore seemingly nonexistent. Ever since then, the study of these miniscule details has brought science into a forensic world once ruled by intuition and deduction. Choosing a microscope with the right optics can reduce these hidden errors considerably to provide results that are both more accurate and more reproducible – two attributes that are both essential in modern forensics.
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  • Using Telecentric Optical Systems to Optimize Industrial Image Accuracy and Reproducibility

    When the first multi-lens microscopes were invented in 1590, scientists marveled at their new ability to SEE small objects and features in the natural world that were previously invisible to the eye and therefore seemingly nonexistent. With the constant miniaturization of parts and products in automated manufacturing over the past 5 decades, the use of microscopes has spread increasingly from science to industry. Today microscopes are found in a multitude of assembly and inspection applications wherever visualization and measurement of miniscule features are required.
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  • Webinar: The Best of Both Worlds: Combining Light Sheet and Confocal Microscopy

    Living cells and organisms often suffer from high light intensities that are used in conventional imaging. Light sheet microscopy reduces phototoxic effects and bleaching, by only illuminating a specimen in a single plane at a time whilst the signal is detected in a perpendicular direction. In combination with high-speed cameras for image acquisition, light sheet microscopy is a very gentle method to observe fast biological processes in sensitive organisms over an extended time period.
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  • Confocal and Digital Light Sheet Imaging

    Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the limited depth of field. Our eye-lens (an optical imaging instrument) has the same trouble, but our brain smartly removes all not-in-focus information before the signal reaches conscious cognition.
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  • Webinar: Light Sheet Imaging – New Solutions and Their Applications in Zebrafish Embryogenesis

    Living cells and organisms often suffer from high light intensities that are used in conventional imaging. Light sheet microscopy reduces phototoxic effects and bleaching, by only illuminating a specimen in a single plane at a time whilst the signal is detected in a perpendicular direction. In combination with high-speed cameras for image acquisition, light sheet microscopy is a very gentle method to observe fast biological processes in sensitive organisms over an extended time period.
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  • Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points

    Cancer research unsing multiphoton microscopy and 3D electron microscopy. Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient events at high resolution. Performing correlative microscopy in complex and bulky samples such as an entire living organism is a time-consuming and error-prone task.
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  • Third Harmonic Generation Microscopy – Label-Free 3D-Tissue Imaging and Blood Flow Characterization

    THG microscopy as special variants of multiphoton microscopy. Third Harmonic Generation (THG) microscopy is a non-fluorescent multi-photon technique that combines the advantages of label-free imaging with restriction of signal generation to the focal spot of the scanning laser. It allows three-dimensional imaging of refraction index mismatches and of hemoglobin.
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  • Nanoscale or Microscale Structures Formed in Polymers Containing Nanotubes Greatly Enhance the Electrical Conductivity: Potential Applications for Photovoltaic Devices

    The excellent mechanical and electrical properties of carbon nanotubes have led to them being exploited for the creation of a new class of high performance polymer composites. Due to important advances in the last few years, nanotube containing polymers have been developed for optoelectronic applications.
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  • Webinar: Light Sheet Imaging for Fast 3D Live Cell and Tissue Imaging

    In recent years, light sheet microscopy has emerged as the biological imaging modality of choice to achieve high performance in imaging speed, resolution, and penetration depth – all with minimal photo-induced damage.
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  • Nature Methods: Light-sheet Fluorescence Microscopy - Method of the Year 2014

    Just about everyone who has examined fluorescent samples under the microscope is aware of the constant struggle to have enough signal to see the labeled structures while also avoiding fluorophore bleaching. What may be less apparent, at least to those who image bright, robust or fixed samples, is how stressful and potentially toxic to living cells and tissues it is to illuminate them with high-intensity light.
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  • 3D STED (Stimulated Emission Depletion) Microscopy

    The resolution needed to image subcellular architecture and dynamics in light microscopy is hindered by the diffraction limits as described by Ernst Abbe. Simply stated, structures smaller than 200 nanometers are lost in a blur. However, the field of super-resolution microscopy has produced methods to obtain resolution beyond this limit. Leica Microsystems has pioneered this field and offers the Leica TCS SP8 STED 3X for 3D Stimulated Emission Depletion microscopy. STED instantly produces super-resolution images, compatible with the dynamics of living cells, without the need for post-processing.
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  • Video Interview with Timo Zimmermann

    The first super-resolution image he saw was an eye opener for him: "It was not just structures that got smaller. I was looking at a sample that I specifically had high hopes of seeing another layer of complexity and this actually was there."
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  • Webinar: New Dimensions in Super-Resolution Microscopy

    This webinar will highlight ground-breaking techniques in microscopy. Two scientists will present the impressive results they have achieved using confocal and widefield 3D super-resolution methods. Dr. Timo Zimmermann, Head of the Advanced Light Microscopy Unit, CRG – Centre for Genomic Regulation, Barcelona, Spain, will present biological applications for the next generation of STED microscopy systems. Dr. Eric Hosy from CNRS, University of Bordeaux, France, will talk about live cell localization microscopy with Leica SR GSD 3D.
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  • Video Talk by Ernst Stelzer: Light Sheet Sectioning

    This talk discusses the technique of light sheet microscopy, also known as selective plane illumination (SPIM). This uses two objectives, one to illuminate the sample and a second to image it and allows long-term 3D imaging of thick specimens like developing embryos with minimal photobleaching and phototoxicity.
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  • Laser Microdissection (LMD) and Fancy Applications

    New and far-reaching applications have recently been opened up in the field of laser microdissection. In addition to conventional dissection, the laser microdissection system (LMD) of Leica Microsystems is an excellent tool for marking relevant structures, offering highly specific laser manipulation of selected areas. This laser marking function is useful for applications such as CLEM, NanoSIMS as well as in the live cell sector.
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  • Three-Dimensional Super-Resolution GSDIM Microscopy

    With the new 3D GSDIM technique structures like the Golgi and the microtubular network are resolved not only laterally, but also in a third dimension. The principle is based on the use of optical astigmatism to determine the accurate lateral and axial position of individual fluorochromes.
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  • From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy

    To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines confocal time-lapse images of GFP-tagged proteins with electron microscopy. With laser micro-patterned culture substrate, we created coordinates that were conserved at every step of the sample preparation and visualization processes. Specifically designed for cryo-fixation, this method allowed a fast freezing of dynamic events within seconds and their ultrastructural characterization.
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  • Webinar: From Epifluorescence to Super-Resolution in 3D

    This webinar will illustrate results obtained by biochemical, Epifluorescence, TIRFM, Confocal and GSD techniques. Depending on the aim of experimental question, different imaging techniques deliver insights into varying aspects of intracellular pathways. To achieve "True-to-detail imaging" of the spatial arrangement of proteins and other biomolecules in cells, GSDIM achieves resolutions up to 20 nm in x and y direction – beyond the diffraction limit of light microscopy. But Super-resolution microscopy can be applied in the axial (z-) direction, too. A recent commercial implementation of the astigmatism approach will be discussed in more detail during this webinar.
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  • New Opportunities for 3D Technology in Medicine

    Until now, physicians have largely been skeptical of the advantages of 3D technology. But this may be about to change: the findings of a new study show that even experienced surgeons stand to benefit from the third dimension.
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  • Light Sheet Fluorescence Microscopy: Beyond the Flatlands

    Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under the name of Ultramicroscopy. Light sheet microscopy utilises a plane of light to optically section samples. This allows deep imaging within transparent tissues and whole organisms. This book chapter will provide the reader with a comprehensive view on this emerging technology.
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  • Tips & Tricks for Using Digital Microscopes

    Digital microscopes provide new opportunities and enhance workflow for measurement and documentation in quality control in 2D and 3D applications. Here, you can find a collection of videos showing set-up tips and tricks for optimal image acquisition. Learn about the use of the zoom lens, the BGA lens, the 360° rotary head, the inclinable stand and the installation of a camera.
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  • Digital Microscopy

    Digital microscopy offers clear advantages for a large number of industrial quality inspections, particularly for surface analysis. Here, you can find some videos that show examples of application for digital microscopy.
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  • Webinar: Challenges in Live Cell Imaging

    Live cell imaging is one tool in the cell biologist's tool box. There are, however, a variety of technologies and methods to achieve this. In this webinar, three practicing researchers discuss: Their use of live cell imaging and how it has helped them achieve publishable results.
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  • Developments in Multiphoton Excitation Microscopy

    Basics, history, and applications of multiphoton microscopy. Honouring Goeppert-Mayer’s prediction of simultaneous two-photon absorption by an atom or molecule reported in the 1930s in her PhD dissertation thesis, we can state that multiphoton excitation (MPE) microscopy, more frequently identified with two-photon excitation (2PE) fluorescence microscopy, is a key microscopy method in many areas from medicine to biology, from biophysics to materials science.
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  • 3D Visualization of Surface Structures

    One of the main features of a digital microscope is the speed and ease with which it enables surface models to be created of macroscopic and microscopic structures. In a qualitative evaluation, these provide a better understanding and a more detailed documentation of the specimen. In addition, quantification of the surface provides valuable information about the composition of the surface and its wear. Which specimens are suitable for use with a Leica digital microscope, and what are the limitations of the method used?
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