Cell Culture
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How can PAULA help streamline the workflow for my wound healing assay?
The wound healing assay, often used as a synonym for scratch assay or migration assay, is an important tool for the evaluation and measurement of cell migration, whether single cells or cell…Read article
Confluency Check with PAULA Cell Imager
Many cell-based experiments require cells in a certain state. This can include their morphology, fluorescent protein expression, and the confluency of the cell layer. Researchers check their cells…Read article
Introduction to Mammalian Cell Culture
Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research…Read article
Visualizing Tropoelastin in a Long-Term Human Elastic Fibre Cell Culture Model
Elastin is an essential protein found in a variety of tissues where resilience and flexibility are needed, such as the skin and the heart. When aiming to engineer suitable implants, elastic fibres are…Read articleHow to do a Proper Cell Culture Quick Check
In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth…Read article
Detailed Morphological Characterisation of Hendra Virus Infection of Different Cell Types Using Super-Resolution and Conventional Imaging
Hendra virus (HeV) is a pleomorphic virus belonging to the Paramyxovirus family. Our long-term aim is to understand the process of assembly of HeV virions. As a first step, we sought to determine the…Read article
Dry Ultrathin Sectioning Combined With High Pressure Freezing
We have used cultured UMR106-01 osteoblastic cells to investigate the process of bone mineralization. UMR106-01 cells as well as primary calvarial bone cells assembly spherical extracellular…Read article
How to Study Protein Recruitment to DNA Lesions by a Combination of UV Laser and White Light Laser
Understanding how DNA lesions are optimally repaired is of functional significance, especially from the view of genome karyotype stability.Read article
Cell Cultures and Laser Microdissection
Many of the discoveries that are now being made in cell division and differentiation, the relationships between single cells and cell organelles, treatments of cells with pharmaceutic substances, etc.…Read article
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further…Read article