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Cryo CLEM

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  • Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy as a Straightforward Tool to Study Host-Pathogen Interactions

    Correlative light and electron microscopy is an imaging technique that enables identification and targeting of fluorescently tagged structures with subsequent imaging at near-to-nanometer resolution. We established a novel correlative cryo-fluorescence microscopy and cryo-scanning electron microscopy workflow, which enables imaging of the studied object of interest very close to its natural state, devoid of artifacts caused for instance by slow chemical fixation. This system was tested by investigating the interaction of the zoonotic bacterium Borrelia burgdorferi with two mammalian cell lines of neural origin in order to broaden our knowledge about the cell-association mechanisms that precedes the entry of the bacteria into the cell.
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  • Webinar: Introduction to Correlative Light and Electron Microscopy (CLEM)

    The webinar will provide an overview of the latest advances in Cryo CLEM, which acts as a powerful interface by combining the best of the light and electron microscopy worlds to overcome their independent barriers and determine the location of fluorescent labelled structures within the landscape of an electron micrograph and showcase how Cryo CLEM adds additional value to quantitative 3D imaging and tomography.
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  • Imaging of Host Cell-bacteria Interactions using Correlative Microscopy under Cryo-conditions

    Pathogenic bacteria have developed intriguing strategies to establish and promote infections in their respective hosts. Most bacterial pathogens initiate infectious diseases by adhering to host cells surface. Knowledge of the interplay between the pathogenic organism and the host cells can provide fundamental new insight into the underlying mechanisms of the infectious process and therefore of the disease. Various microscopy techniques have proven to be critical tools to study these events.
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  • Webinar: Correlative Fluorescence and (Cryo-) Electron Microscopy

    In this webinar, you will learn how you can identify objects of interest based on their fluorescent signal, and then find and image them in 3D in the transmission electron microscope using correlative light and electron microscopy (CLEM) methods.
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  • Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins

    We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer superior structural preservation, performing in-resin super-resolution, however, remains a challenge.
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  • Cryo CLEM – the Combination of Cryo Fluorescence Microscopy with Cryo Electron Microscopy

    Many biological insights can be obtained by combining the power of Fluorescence Microscopy (FM) with that of Electron Microscopy (EM) to study the same sample – this is called Correlative Light and Electron Microscopy (CLEM). In FM, specific proteins can be labelled and identified, and their dynamics and interactions can be visualized in fixed or living cells.
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