CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…
More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…
Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…
The colorful picture shows colon tumor cells, fluorescently labelled and lineage traced from a multicolor tracer. The gray color codes for the second harmonic generation (SHG) signal from Collagen 1.…
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…
Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of the whole brain to the study of tumor development…
Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective.
Understanding kidney microanatomy is key to detecting and identifying early events in kidney…
Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing interest in this…
Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for…
Cancer research unsing multiphoton microscopy and 3D electron microscopy.
Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient…
CLARITY clearing derivatives for multiphoton microscopy.
Transparent organisms help us to identify spatial arrangements and connections of cells and tissues, especially neuronal circuits can easily…
Multi-channel multiphoton microscopy with dedicated optics for CLARITY.
Why clearing? Curiosity is human nature. And nothing attracts as much curiosity as the inside of living organisms. While in…
Imaging whole brains with CLARITY and multiphoton microscopy.
Image a whole brain without sectioning? Investigate neuronal circuits without reconstruction? Perform molecular phenotyping without…
Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under the name of Ultramicroscopy. Light sheet…
Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This…