A new type of fluorescent bead is presented by GATTAquant. These beads, called GATTA-Beads, are characterized by a small diameter (23 nm), high intensity and size uniformity. In combination with state-of the-art
STED microscopes such as the Leica
TCS SP8
STED 3X and high-end image restoration methods available in the Huygens Software, it is shown that these new beads can be used for accurate
STED PSF characterization in 3D. Furthermore, it is shown that the measured 3D
STED-PSF can be used to improve image restoration quality in combination with
STED deconvolution methods available in the Huygens Software.
