Fluorescence Lifetime Imaging (FLIM
) in combination with Förster Resonance Energy Transfer (FRET
) has proven to be very beneficial for investigations in biomedical research for a wide range of structural elements and dynamic changes in cells. FRET
allows to monitor molecular interactions, as the FRET
signal depends strongly on the distance of the two FRET
partners. This allows to investigate interaction of molecules, like ligand-receptor pairs, protein-protein interactions or interactions of effectors with DNA.