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  • Real Time Observation of Neutrophil White Blood Cell Recruitment to Bacterial Infection In Vivo

    The zebrafish (Danio rerio) is an emerging vertebrate model organism to study infection. The transparent larva comprises a fully functional innate immune system and enables live imaging of fluorescent immune cells in transgenic animals. Zebrafish infection models have been developed for both the human bacterial pathogen Shigella flexneri and the natural fish bacterial pathogen Mycobacterium marinum. Importantly, whilst S. flexneri causes acute infection and is typically used as an inflammatory paradigm, M. marinum causes a chronic disease similar to tuberculosis in humans. Here, we use real time fluorescence microscopy to image transgenic zebrafish larvae with neutrophils (granulocyte white blood cells) expressing the green fluorescent protein eGFP.
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  • Characterization of Photosynthetic Biofilms from Roman Catacombs via 3D Imaging and Subcellular Identification of Pigments

    Artificial illumination can harm hypogean monuments by inducing the uncontrolled growth of photosynthetic biofilms (green sickness or maladie verte). With the aim of preventing biodeterioration or aesthetic damage in Roman hypogean monuments (the St. Callistus and St. Domitilla catacombs, Rome, Italy), a confocal technique is used for the analysis of fluorescent pigments from a single cell, based on spectrofluorometric methods.
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