The resolution needed to image subcellular architecture and dynamics in light microscopy is hindered by the diffraction limits as described by Ernst Abbe. Simply stated, structures smaller than 200 nanometers are lost in a blur. However, the field of super-resolution microscopy has produced methods to obtain resolution beyond this limit. Leica Microsystems has pioneered this field and offers the Leica TCS
3X for 3D Stimulated Emission Depletion microscopy. STED
instantly produces super-resolution images, compatible with the dynamics of living cells, without the need for post-processing.