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Phase Contrast

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  • The Principles of Phase Contrast

    In this tutorial the principle of phase contrast imaging is described taking the example of an inverted research microscope. Additionally, the alignment of the components needed for phase contrast is shown in the interactive part of the tutorial.
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  • Cell Cultures and Laser Microdissection

    Many of the discoveries that are now being made in cell division and differentiation, the relationships between single cells and cell organelles, treatments of cells with pharmaceutic substances, etc. would not be possible without live cell cultures. Allowing morphological and biochemical observations of single cells under different experimental conditions, they provide a unique source of information.
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  • Optical Contrast Methods

    Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the specimen into amplitude shifts that are visible to the human eye as differences in brightness.
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  • Phase Contrast

    Phase contrast is an optical contrast technique for making unstained phase objects (e.g. flat cells) visible under the light microscope. Cells that appear inconspicuous and transparent in brightfield can be viewed in high contrast and rich detail using phase contrast microscopy.
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