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  • What Makes sCMOS Microscope Cameras so Popular?

    sCMOS cameras are more sensitive and are capable of much higher acquisition speed than cameras with other sensor types. Even though CCD cameras are widely used in live cell imaging and time-lapse recordings, researchers are often concerned that their camera does not detect faint signals. In this interview, Dr. Karin Schwab, Product Manager at Leica Microsystems, talks about the characteristics of sCMOS cameras and how researchers benefit from the latest camera sensor technology.
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  • Light Sheet Microscopy Turned Vertically

    Living cells and organisms often suffer from the high light intensities used for fluorescent imaging. Light sheet microscopy reduces phototoxic effects and bleaching by illuminating a specimen in only a single plane at a time. A new light sheet microscope combines light sheet and confocal microscopy in one system without compromising either functionality and allows the combination of the two methods, e.g. confocal photomanipulation with subsequent light sheet acquisition, for new applications.
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  • Video Talk by Ernst Stelzer: Light Sheet Sectioning

    This talk discusses the technique of light sheet microscopy, also known as selective plane illumination (SPIM). This uses two objectives, one to illuminate the sample and a second to image it and allows long-term 3D imaging of thick specimens like developing embryos with minimal photobleaching and phototoxicity.
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  • Principles of Multiphoton Microscopy for Deep Tissue Imaging

    Basics of multiphoton microscopy. This interactive tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking advantage of the reduced scattering of longer wavelengths. This makes multiphoton imaging the perfect tool for deep tissue imaging in thick sections and living animals.
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  • Live-Cell Imaging

    The understanding of complex and fast cellular dynamics is an important step to get insight into biological processes. Therefore, today’s life science research more and more demands studying physiological events on the molecular level in real-time.
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  • Step by Step Guide to the Molecular Basics of CARS Microscopy

    CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial advantage of this method is that the sample remains almost unaffected. This tutorial explains the molecular basics on Coherent Anti-Stokes Raman Scattering.
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  • Step by Step Guide to Hybrid Detection and Photon Counting

    This tutorial explains the underlying hybrid detection technology and compares it to photomultiplier technology. The implications of hybrid detection design for imaging and photon counting are discussed. The tutorial closes with a brief summary of photon counting in the context of imaging.
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  • Brighter Fluorescence by Resonant Scanning

    Fast True Confocal Scanning reduces photobleaching and increases the fluorescence yield at identical acquisition times. The long-lasting triplet state (or any other “dark state”) is less populated when the illumination is applied in shorter pulses at the same intensity. Consequently, more fluorochromes are available for the fluorescence process (brighter images) and fewer fluorochromes disintegrate from triplet states or excited triplet states (less bleaching).
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