Point Spread Function (PSF)
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Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…Read article
Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images
Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution. This is typically obtained by measuring the image of…Read article
Methods to Calibrate and Scale Axial Distances in Confocal Microscopy as a Function of Refractive Index
Application example of HyVolution Super-Resolution - Accurate distance measurement in 3D confocal microscopy is important for quantitative analysis, volume visualization and image restoration.…Read article
Measuring the 3D STED-PSF with a new Type of Fluorescent Beads
A new type of fluorescent bead is presented by GATTAquant. These beads, called GATTA-Beads, are characterized by a small diameter (23 nm), high intensity and size uniformity. In combination with…Read article
Mirror-Enhanced Super-Resolution Microscopy
Axial excitation confinement beyond the diffraction limit is crucial to the development of next-generation, super-resolution microscopy. STimulated Emission Depletion (STED) nanoscopy offers lateral…Read article
Translation Microscopy (TRAM) for Super-Resolution Imaging
Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We…Read article
Quantifying the Resolution of a Leica SR GSD 3D Localization Microscopy System with 2D and 3D Nanorulers
DNA origami based nanorulers produced by GATTAquant are common standards to test the achievable spatial resolution of super-resolution microscopes. Recently the nanorulers were used to test the…Read article
Improving Axial Resolution in Confocal Microscopy with New High Refractive Index Mounting Media
Resolution, high signal intensity and elevated signal to noise ratio (SNR) are key issues for biologists who aim at studying the localisation of biological structures at the cellular and subcellular…Read article
Super-Resolution – On a Heuristic Point of View About the Resolution of a Light Microscope
Since super-resolution has become one of the most favored methods in biomedical research, the term has become increasingly popular. Still, there is much of confusion about what is super-resolution and…Read article
Video Talk by Jeff Lichtman: Point Spread Function
An infinitesimally small point appears in the microscope as a spot with a certain size, blurred in the z-direction and with concentric rings around it. This "point spread function" reveals many of the…Read article
Pinhole Geometry: Four Corners are Perfect
Square and hexagonal pinholes provide identical image signal levels, if the geometries are compared in a sensible manner. The amount of light passing the pinhole depends on the area of that aperture,…Read article
Image Processing for Widefield Microscopy
Fluorescence microscopy is a modern and steadily evolving tool to bring light to current cell biological questions. With the help of fluorescent proteins or dyes it is possible to make discrete…Read article
A Guide to Super-Resolution Fluorescence Microscopy
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass…Read article