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Quantitative Microscopy

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  • Webinar: Introduction to Correlative Light and Electron Microscopy (CLEM)

    The webinar will provide an overview of the latest advances in Cryo CLEM, which acts as a powerful interface by combining the best of the light and electron microscopy worlds to overcome their independent barriers and determine the location of fluorescent labelled structures within the landscape of an electron micrograph and showcase how Cryo CLEM adds additional value to quantitative 3D imaging and tomography.
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  • Webinar: Leveraging Digital Microscopy to Increase Overall Throughput and Efficiency

    The Digital Microscope has rapidly evolved from an emerging technology to the industry standard for quality-control, failure analysis and R&D inspection / measurement in various disciplines, such as Medical Devices, Plastics, Automotive, Aerospace, and Electronics manufacturing. As more companies in these markets demand increased product quality and faster time-to-result, while investing less time and money in advanced microscopy training, the Digital Microscope helps achieve these goals.
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  • Novel Microscopy-Based Screening Method Reveals Regulators of Contact-Dependent Intercellular Transfer

    Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators.
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  • Using Telecentric Optical Systems to Optimize Forensic Image Accuracy and Reproducibility

    When the first compound microscopes were invented in 1590, scientists marveled at their new ability to see tiny objects and features that were previously invisible to the eye and therefore seemingly nonexistent. Ever since then, the study of these miniscule details has brought science into a forensic world once ruled by intuition and deduction. Choosing a microscope with the right optics can reduce these hidden errors considerably to provide results that are both more accurate and more reproducible – two attributes that are both essential in modern forensics.
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  • Using Telecentric Optical Systems to Optimize Industrial Image Accuracy and Reproducibility

    When the first multi-lens microscopes were invented in 1590, scientists marveled at their new ability to SEE small objects and features in the natural world that were previously invisible to the eye and therefore seemingly nonexistent. With the constant miniaturization of parts and products in automated manufacturing over the past 5 decades, the use of microscopes has spread increasingly from science to industry. Today microscopes are found in a multitude of assembly and inspection applications wherever visualization and measurement of miniscule features are required.
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  • What You Always Wanted to Know About Digital Microscopy, but Never Got Around to Asking

    Digital microscopy is one of the buzz words in microscopy – and there are a couple of facts that are useful to know. Georg Schlaffer, Product Manager with Leica Microsystems, has often been asked about digital microscopy by customers and colleagues alike. He has worked together with Scientific Writer Jim DeRose for comprehensive answers to the most important ones.
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  • Are Your Digital Microscope Measurements Accurate and Reliable?

    For certain applications, such as the production and maintenance of automobiles, aircraft, or power plants, quality control and reliability assurance, as well as safety and health inspection, accurate and reliable image data with precise calibration are very important. Digital microscopes are appealing for a wide range of technical applications in various industries, such as automotive, aerospace, precision engineering, microelectronics, and medical devices.
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  • Fully Automated Tracking of Chloroplasts in Elodea Leaf Cells from 4D Image Data

    Fully automated tracking of moving 3D structures in living cells represents several challenges that are typical to the analysis of biological image data. Taking 4D image data from chloroplasts in living Elodea leaf cells as an example we demonstrate new approaches to meet these challenges. Chloroplasts are surprisingly dynamic organelles: They move extensively throughout cells, they regularly split or fuse and their shape changes constantly.
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  • Image Cytometry

    Microscopy-based imaging is booming and the need for tools to retrieve quantitative data from images is urgent. This book provides simple but reliable tools to generate valid quantitative gene expression data, at the mRNA, protein and activity level, from microscopic images in relation to structures in cells, tissues and organs in 2D and 3D.
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  • 100 Years of Binoculars and Quantitative Microscopy

    One hundred years ago, in 1913, the Optische Werke Ernst Leitz in Wetzlar, predecessor of Leica Microsystems CMS GmbH, made two inventions that were to blaze the trail for modern microscopy: the binocular tube and the integrating stage for quantitative microscopy.
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  • Integrative Open-Source Software for Image Analysis in Biology

    Imaging techniques are indispensable in many fields of life sciences today. With state-of-the-art optics and metrology, they provide hundreds of gigabytes of still images and videos. Correspondingly, there is a growing need for complex software solutions to ensure that the amounts of generated data can be automatically managed, processed and analyzed – and shared online with a large group of users. The combination of individual open-source software projects is proving especially useful for solving such complex image analysis problems.
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  • 50 Years of Image Analysis

    Modern image analysis systems perform highly sophisticated image processing functions on images from an automated microscope and digital camera. 50 years ago, the first image analysis system was analogue, based on a video camera and the area measurements could be read from a meter. Nevertheless, it marked the beginning of automation in this field.
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  • Research for the Optimal Structure

    To see how liquids can be made to flow, without being directly heated or touched, you only have to watch a raw egg explode in a microwave oven. Electromagnetic forces can even melt metal at hotter than 1000 °C. In the Magnetohydrodynamics study group at the Research Centre Dresden-Rossendorf (FZD) these complex interactions between electrically conductive liquids and magnetic fields are used to control the flow and solidification processes of liquid metal alloys.
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  • A New FRAP/FRAPa Method for Three-Dimensional Diffusion Measurements Based on Multiphoton Excitation Microscopy

    Quantitative measurement method based on FRAP and FRAPa using multiphoton microscopy. We present a new convenient method for quantitative three-dimensionally resolved diffusion measurements based on the photobleaching (FRAP) or photoactivation (FRAPa) of a disk-shaped area by the scanning laser beam of a multiphoton microscope. Contrary to previously reported spot-photobleaching protocols, this method has the advantage of full scalability of the size of the photobleached area and thus the range of diffusion coefficients, which can be measured conveniently.
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