In this webinar, we show that optical clearing drastically increases the signal-to-noise ratio and staining quality, thus enabling STED
nanoscopy of the subtlest elements of the kidney. In this way we show that optical clearing is not only a sample preparation technique to consider when imaging large mm-scale samples, but could also be fruitful when imaging at the nanoscale. Furthermore, the increased transparency of the optically cleared sample enables volumetric 3D STED
imaging at sub-diffraction-limited resolution.