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  • See the Structure with Microscopy - Know the Composition with Laser Spectroscopy

    The advantages of a 2-in-1 materials analysis solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) for simultaneous visual and chemical inspection are described in this report. The basic principles of the 2-in-1 solution and a comparison between it and other common materials analysis methods, such scanning electron microscopy (SEM), are explained to demonstrate how a rapid, efficient workflow is achieved. A 2-in-1 analysis solution can reduce significantly the cost and time for obtaining material image and composition data. Such data are instrumental in assuring quality and reliability to make confident decisions quickly during production, quality control, failure analysis, and research and development in industries and fields, such as automotive and metallurgy.
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  • How to Turn Microscope Workplaces Ergonomic

    Microscopes are tools that affect those who work with them every day. They can be highly demanding for the human body, requiring concentration and a lot of steady activity from many of our muscles. In this interview, Clinton Smith, Senior Product Manager at Leica Microsystems, talks about how to relieve possible tension and strain and how to create ergonomic workplaces to help microscope users work in comfort and how to increase productivity.
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  • How Digital Microscopy can Contribute to Efficient Workflows for Microelectronics and Electronics

    This report explains how users can benefit from the digital microscope portfolio of Leica Microsystems to attain cost-effectiveness over entire workflows in research and development (R&D), product innovation, process engineering, production, quality control and assurance (QC/QA), and failure analysis (FA).
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  • Work More Efficiently in Developmental Biology With Stereo Microscopy: Zebrafish, Medaka, and Xenopus

    Among the aquatic model organisms used in molecular and developmental biology the most prominent are the zebrafish (genus species: Danio rerio), medaka or japanese rice fish (genus species: Oryzias latipes), and african clawed frog (genus species: Xenopus laevis). This report gives useful information to scientists and technicians which can help improve their daily laboratory work by making the steps of transgenesis, fluorescent screening, and functional imaging more efficient.
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  • Practical Guide for Excellent GSDIM Super-Resolution Images

    Do you know that most protists and bacteria lack in one feature that each of our body cell has? Our cells are touch and communicate with one another. They send and receive a variety of signals that coordinate their behavior to act together as a functional multicellular organism. Exploring the way of cellular communication and the ways how the cell surface interacts to organize tissues and body structures is of great interest. Kees Jalink and his team of scientists at the Netherlands Cancer Institute (NKI) in Amsterdam obtained new scientific insights into the molecular architecture of hemidesmosomes, cytoskeletal components, cell surface receptors and vesicular proteins with the help of Ground-State-Depletion (GSD)/ dSTORM microscopy. In this interview, Kees Jalink comments on their developments in imaging chambers, buffer conditions and image analysis to get the perfect super resolution image.
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  • Forensics: How to Gather Evidence from Ballistics, Tool Marks, Questioned Documents, Counterfeiting, and Forgery Fast and Easily with Digital Microscopy

    The examples reported here demonstrate how digital microscopy enables forensic scientists to gather and compare evidence from ballistics and tool marks or counterfeited money, credit cards, and documents, more efficiently. Images and videos clearly show how to use the digital microscope to perform forensics investigations and how you speed up your workflow with the help of practical features, such as a convenient way to change magnification rapidly over the full range, tilting, sample rotation, and versatile illumination.
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  • C. Elegans

    Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans

    For scientists, technicians, and teachers working with the worm C. elegans in the research lab or classroom, this report is intended to give useful information to help improve their daly work. The aim is to make the work steps of worm picking, transgenesis, RNA interference, screening, and functional imaging efficient. It also details the various possibilities for equipping a research worm lab or biology classroom/teaching lab explaining worm methods.
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  • Workflows & Protocols: Plant Laser Microdissection

    During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection using the Leica LMD systems was demonstrated. One special focus was on plant dissection which needs a high laser power.
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  • Circuit board inspection

    Fast and Reliable Inspection of Printed Circuit Boards with Digital Microscopy

    Digital microscopy has been used more and more for inspection, quality control and assurance (QC/QA), failure analysis (FA), and research and development (R&D) in the microelectronics industry, especially for printed circuit boards (PCBs). Digital microscopes are practical to use and allow an efficient workflow for inspection.
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  • Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research

    Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a perfect tool for this purpose and how this was taught in the course of a workshop held in Brazil. With the Leica LMD system pure tumor material can be selected and dissected for downstream analysis to ensure 100% pure starting material without any risk of cross contamination with healthy cells.
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  • Automotive Industry: How Suppliers and Auto Manufacturers Can Verify Parts Specifications Quickly and Easily

    Checking specifications is critical: During the manufacture of auto parts, specifications must be met – whether by the auto parts supplier or the automobile manufacturer. It is important that the parts meet specifications as they are critical for maintaining the performance standards and safe operation of automobiles, trucks, and other vehicles during their lifetimes.
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  • Webinar: Sample Preparation of Nanocomposites and Nanomaterials by Ultramicrotomy - a Powerful Alternative to FIB

    In this webinar the sample preparation workflow including the Ultramicrotome Leica EM UC7, its cryo-chamber Leica EM FC7 and the pre-preparation system Leica EM TXP will be given. The main part of this webinar will cover tips and tricks to reveal the internal structure of composites and materials being investigated with TEM and STEM. Differences between Focused Ion Beam (FIB) and Ultramicrotomed samples will be shown and explained.
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  • How to do a Proper Cell Culture Quick Check

    In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth must be monitored at regular intervals. This article describes a typical workflow for subculturing an adherent cell line with detailed illustrations of all of the necessary steps.
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  • Inspecting and Analyzing Printed Circuit Boards Quickly and Reliably with a Digital Microscope

    For the past several years, digital microscopy has been shown to be useful for inspection, quality control and assurance (QC/QA), and failure analysis (FA) in the microelectronics industry, especially for printed circuit boards (PCBs). Recently, state-of-the-art improvements have made digital microscopy even more powerful and practical for inspection, leading to a more efficient workflow. Here, the advantages of certain digital microscope features, i.e., intuitive software for operation and analysis, fast and easy ways to change magnification, and encoding for reliable recall of parameters, are explained.
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  • Automotive Industry: Rapid and Precise Surface Inspection on Hard-to-Image Samples

    In the automotive industry, microscopists may be challenged with samples that are difficult to image, for example, parts that have a special material composition. This report shows how the Leica DVM6 digital microscope helps to make inspection, measurement analysis, and reporting of such challenging samples quicker and easier.
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  • Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience

    The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as it allows to separate distinct brain layers or even to isolate individual neurons.
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  • Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection

    During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome. Another topic was the dissection of individual chromosomes from chromosome spreads. Leica specialists held a short training session with the LMD. After this, new LMD users were able to run the system and practiced how to dissect chromosomes and collect single chromosomes for downstream analysis.
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  • Webinar: Leveraging Digital Microscopy to Increase Overall Throughput and Efficiency

    The Digital Microscope has rapidly evolved from an emerging technology to the industry standard for quality-control, failure analysis and R&D inspection / measurement in various disciplines, such as Medical Devices, Plastics, Automotive, Aerospace, and Electronics manufacturing. As more companies in these markets demand increased product quality and faster time-to-result, while investing less time and money in advanced microscopy training, the Digital Microscope helps achieve these goals.
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  • Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis

    Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or sequencing techniques. Here we describe the successful combination of the Leica Microsystems LMD system and Qiagen kits for purification of nucleic acids even from little amounts.
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  • What You Always Wanted to Know About Digital Microscopy, but Never Got Around to Asking

    Digital microscopy is one of the buzz words in microscopy – and there are a couple of facts that are useful to know. Georg Schlaffer, Product Manager with Leica Microsystems, has often been asked about digital microscopy by customers and colleagues alike. He has worked together with Scientific Writer Jim DeRose for comprehensive answers to the most important ones.
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  • Five Advantages of Inverted Over Upright Microscopes in Industrial Applications

    With inverted microscopes, you look at samples from below since their optics are placed under the sample, with upright microscopes you look at samples from above. Traditionally, inverted microscopes are used for life science research, because gravity makes samples sink to the bottom of a holder with aqueous solution and you don’t see a lot from above.
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  • Are Your Digital Microscope Measurements Accurate and Reliable?

    For certain applications, such as the production and maintenance of automobiles, aircraft, or power plants, quality control and reliability assurance, as well as safety and health inspection, accurate and reliable image data with precise calibration are very important. Digital microscopes are appealing for a wide range of technical applications in various industries, such as automotive, aerospace, precision engineering, microelectronics, and medical devices.
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  • Webinar: Increase Efficiency and Productivity at your Microscope Workstation

    Ergonomic workstations improve more than comfort – they improve the overall wellbeing of the work force. With a few simple adjustments and the right equipment to provide better posture, microscope users can dramatically increase productivity, focus, and efficiency on the job, while reducing health issues in both the short and long term.
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  • Higher Motivation, Longer Concentration – Ergonomics as a Competitive Advantage

    Sensor and identification technology, industrial cameras: when top precision is required, the name Baumer Electric is soon mentioned. The Swiss company designs and produces components for manufacturing facilities all over the world. To be able to guarantee that the finished components are of the highest possible quality, elaborate quality inspections are carried out part for part under the stereo microscope.
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  • Ergonomically Designed Surgical Microscopes Support Performance

    Operations not only demand maximum concentration of the surgeon but also maximum physical fitness. Physiotherapist John Ludescher explains how the body can be given optimal support to perform at its best. The Austrian qualified as physiotherapist in Switzerland and has had his own practice for nine years. He is the author of the book “Aufrecht – Bewusst – Stark” (Upright – Aware – Strong) and designer of the “PhysioCap”.
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  • Integrative Open-Source Software for Image Analysis in Biology

    Imaging techniques are indispensable in many fields of life sciences today. With state-of-the-art optics and metrology, they provide hundreds of gigabytes of still images and videos. Correspondingly, there is a growing need for complex software solutions to ensure that the amounts of generated data can be automatically managed, processed and analyzed – and shared online with a large group of users. The combination of individual open-source software projects is proving especially useful for solving such complex image analysis problems.
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  • Body in Balance

    Nobody is the same. Whether large or small, well- or slightly built, left- or right-handed, everyone has different demands of the tool they work with for many hours a day. This is particularly true of laboratory workstations, where routine tasks such as pipetting or microscope or microtome work require a static posture.
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  • Webinar: Digital Metallographic and Quality-Control Advancements to Help Increase Efficiency

    Doing more with fewer resources is today’s standard, and the metallurgical/quality-control laboratory is no exception. Digitizing the metallurgical laboratory provides software tools that increase overall efficiency and accuracy of microstructure evaluation in accordance with ASTM and ISO standards, automate the generation of quality reports, and provide a structured interface for archiving images and related data. This webinar will provide a comprehensive overview of how these digital tools can help optimize workflow and save time and money.
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