Leica Science Lab - Tag : Bleaching https://www.leica-microsystems.com//science-lab/tag/tags/bleaching/show/Tag/ Article tagged with Bleaching en-US https://www.leica-microsystems.com/15077 Light Sheet Microscopy Nature Methods: Light-sheet Fluorescence Microscopy - Method of the Year 2014 Just about everyone who has examined fluorescent samples under the microscope is aware of the constant struggle to have enough signal to see the labeled structures while also avoiding fluorophore bleaching. What may be less apparent, at least to those who image bright, robust or fixed samples, is how stressful and potentially toxic to living cells and tissues it is to illuminate them with high-intensity light. https://www.leica-microsystems.com//science-lab/nature-methods-light-sheet-fluorescence-microscopy-method-of-the-year-2014/ Wed, 14 Jan 2015 17:24:00 +0000 https://www.leica-microsystems.com/9759 Quantitative Fluorescence Step by Step Guide for FRAP Experiments Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be used to determine kinetic properties, like the diffusion coefficient, mobile fraction, and transport rate of the fluorescently labeled molecules. FRAP employs irradiation of a fluorophore with a short laser pulse. State of the art laser scanning microscopes, like the TCS SP8 confocal system, have the advantage of using a high intensity laser for photobleaching and a low intensity laser for image recording. With the LAS AF application wizard you can choose between different ways to carry out a FRAP experiment. https://www.leica-microsystems.com//science-lab/step-by-step-guide-for-frap-experiments/ Fri, 17 May 2013 13:50:00 +0000 Dr. Jan Schröder https://www.leica-microsystems.com/5890 Fluorescence Microscopy Basics in Microscopy Basic Principles of Luminescence There are a lot of light-emitting processes occurring in nature. Luminescence is an umbrella term for those kinds of events where light emission is not the result of high temperatures. This article depicts the different forms of luminescences and goes into detail in the case of fluorescence. Relevant technical terms describing a fluorochrome, like quenching, bleaching or quantum yield, are explained in the second part of the article to give detailed insights into basic characteristics of fluorescent molecules. https://www.leica-microsystems.com//science-lab/basic-principles-of-luminescence/ Mon, 09 Apr 2012 22:00:00 +0000 Dr. Christoph Greb