Leica Science Lab - Tag : Blood Vessel https://www.leica-microsystems.com//science-lab/tag/tags/blood-vessel/show/Tag/ Article tagged with Blood Vessel en-US https://www.leica-microsystems.com/20418 Coherent Raman Scattering (CRS) Super-Resolution Multiphoton Microscopy Direct visualization of the arterial wall water permeability barrier using CARS microscopy The artery wall is equipped with a water permeation barrier that allows blood to flow at high pressure without significant water leak. The precise location of this barrier is unknown despite its importance in vascular function and its contribution to many vascular complications when it is compromised. Herein we map the water permeability in intact arteries, using coherent anti-Stokes Raman scattering (CARS) microscopy and isotopic perfusion experiments. Generation of the CARS signal is optimized for water imaging with broadband excitation. https://www.leica-microsystems.com//science-lab/direct-visualization-of-the-arterial-wall-water-permeability-barrier-using-cars-microscopy/ Wed, 22 Aug 2018 22:00:00 +0000 https://www.leica-microsystems.com/18952 Super-Resolution Live-Cell Imaging Image Restoration and Deconvolution Discovery of Novel Peptides Targeting Pro-Atherogenic Endothelium in Disturbed Flow Regions -Targeted siRNA Delivery to Pro-Atherogenic Endothelium in vivo Application example of HyVolution Super-Resolution - Atherosclerosis occurs preferentially in arterial regions exposed to disturbed blood flow. Targeting these pro-atherogenic regions is a potential anti-atherogenic therapeutic approach, but it has been extremely challenging. Here, using in vivo phage display approach and the partial carotid ligation model of flow-induced atherosclerosis in mouse, we identified novel peptides that specifically bind to endothelial cells (ECs) exposed to disturbed flow condition in pro-atherogenic regions. https://www.leica-microsystems.com//science-lab/discovery-of-novel-peptides-targeting-pro-atherogenic-endothelium-in-disturbed-flow-regions-targeted-sirna-delivery-to-pro-atherogenic-endothelium-in-vivo/ Thu, 10 Nov 2016 15:28:00 +0000 https://www.leica-microsystems.com/15430 Multiphoton Microscopy Confocal Microscopy Live-Cell Imaging Third Harmonic Generation Microscopy – Label-Free 3D-Tissue Imaging and Blood Flow Characterization THG microscopy as special variants of multiphoton microscopy. Third Harmonic Generation (THG) microscopy is a non-fluorescent multi-photon technique that combines the advantages of label-free imaging with restriction of signal generation to the focal spot of the scanning laser. It allows three-dimensional imaging of refraction index mismatches and of hemoglobin. https://www.leica-microsystems.com//science-lab/third-harmonic-generation-microscopy-label-free-3d-tissue-imaging-and-blood-flow-characterization/ Sat, 11 Apr 2015 17:34:00 +0000 Dr. Steffen Dietzel https://www.leica-microsystems.com/15424 Stereo Microscopy Egg-in-Cube: Design and Fabrication of a Novel Artificial Eggshell with Functionalized Surface The chick embryo and its circulatory system enclosed by the eggshell has become an important model for biomedical research such as the control of angiogenesis, cancer therapy, and drug delivery test. In this study, a novel artificial eggshell with functionalized surface is proposed, which allows the total amount of oxygen to pass into the egg for the chick embryo culturing and has high observability and accessibility for embryo manipulation. https://www.leica-microsystems.com//science-lab/egg-in-cube-design-and-fabrication-of-a-novel-artificial-eggshell-with-functionalized-surface/ Tue, 07 Apr 2015 15:55:00 +0000 https://www.leica-microsystems.com/9356 Live-Cell Imaging Stereo Microscopy Intravital Immunofluorescence for Visualizing the Microcirculatory and Immune Microenvironments in the Mouse Ear Dermis Intravital imaging of inflammatory and remodeling processes has been an important area of research and has also motivated the creation of numerous transgenic reporter mouse models that express fluorescent proteins. Our paper (Kilarski, Güç et al. 2013) describes a new in vivo imaging technique, which design was based on innovative concept of using immunostaining for live cells and tissue structures on surgically exposed mouse dermis without causing harmful immunotoxic effect. https://www.leica-microsystems.com//science-lab/intravital-immunofluorescence-for-visualizing-the-microcirculatory-and-immune-microenvironments-in-the-mouse-ear-dermis/ Mon, 18 Mar 2013 23:00:00 +0000 PhD Witold W. Kilarski, Esra Güç, PhD Melody A. Swartz