Leica Science Lab - Tag : Brain Tissue https://www.leica-microsystems.com//science-lab/tag/tags/brain-tissue/show/Tag/ Article tagged with Brain Tissue en-US https://www.leica-microsystems.com/25096 Widefield Microscopy Alzheimer Plaques: fast Visualization in Thick Sections More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this disease. A few treatments try to slow down the fatal path or try to relieve the patients from symptoms. The laboratory of Dr. Mehrdad Shamloo at Stanford University studies pathological brain function with the goal of contributing to the discovery of novel therapeutics for Alzheimer’s disease. They use a mouse model of this disease to study the role of inflammation in Alzheimer’s disease progression. This requires imaging of thick uncleared brain tissues. https://www.leica-microsystems.com//science-lab/alzheimer-plaques-fast-visualization-in-thick-sections/ Thu, 27 Jun 2019 10:50:00 +0000 PhD Olga Davydenko, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/18146 EM Sample Preparation Cultured Rat Hippocampal Neurons Application Note for Leica EM ICE - Rat Hippocampal neurons, cultured on 50 μm thick Aclar (Aclar embedding film, EMS) for 19 days, were frozen in the 100 μm deep side of lecithin coated (detailed protocol Appendix I) type A 3 mm Cu/Au carriers (Leica) and sandwiched with the flat side of lecithin coated type B 3 mm Cu/Au carriers (Leica). No additional filler was used, only cell culture medium with the addition of Hepes buffer pH 7.2 to a final concentration of 25 mM. Samples were frozen in a high-pressure freezer (Leica EM ICE). https://www.leica-microsystems.com//science-lab/cultured-rat-hippocampal-neurons/ Mon, 29 Aug 2016 07:12:00 +0000 E. G. van Donselaar, Dr. Martin Harterink, Drs. C. E. M. Vocking, Dr. W. H. Mueller, Prof. Dr. C. C. Hoogenraad https://www.leica-microsystems.com/17957 Super-Resolution Confocal Microscopy Light Sheet Microscopy Neuroscience Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for high-resolution fluorescence imaging (SeeDB2). SeeDB2 matches the refractive indices of fixed tissues to that of immersion oil (1.518), thus minimizing both light scattering and spherical aberrations. https://www.leica-microsystems.com//science-lab/super-resolution-mapping-of-neuronal-circuitry-with-an-index-optimized-clearing-agent/ Wed, 27 Apr 2016 10:07:00 +0000 https://www.leica-microsystems.com/16814 Laser Microdissection Neuroscience Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as it allows to separate distinct brain layers or even to isolate individual neurons. https://www.leica-microsystems.com//science-lab/workflows-protocols-connecting-microscopy-and-molecular-biology-in-neuroscience/ Fri, 04 Dec 2015 16:01:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/15458 Confocal Microscopy Fluorescence Microscopy Improving Axial Resolution in Confocal Microscopy with New High Refractive Index Mounting Media Resolution, high signal intensity and elevated signal to noise ratio (SNR) are key issues for biologists who aim at studying the localisation of biological structures at the cellular and subcellular levels using confocal microscopy. The resolution required to separate sub-cellular biological structures is often near to the resolving power of the microscope. https://www.leica-microsystems.com//science-lab/improving-axial-resolution-in-confocal-microscopy-with-new-high-refractive-index-mounting-media/ Thu, 11 Jun 2015 13:04:00 +0000 https://www.leica-microsystems.com/14402 Multiphoton Microscopy Neuroscience Clearing Procedures for Deep Tissue Imaging Multi-channel multiphoton microscopy with dedicated optics for CLARITY. Why clearing? Curiosity is human nature. And nothing attracts as much curiosity as the inside of living organisms. While in ancient times those who cut human bodies open to do research were put to death, and modern anatomy started only after Pope Clement VII allowed dissection, we can now watch brains working in living animals – and have a good chance of soon being able to interfere with the observed activities for healing (or control) purposes. https://www.leica-microsystems.com//science-lab/clearing-procedures-for-deep-tissue-imaging/ Thu, 18 Sep 2014 12:36:00 +0000 Dr. Rolf T. Borlinghaus, Dr. Andrea Mülter https://www.leica-microsystems.com/12768 Neuroscience Confocal Microscopy Multiphoton Microscopy Map the Brain with CLARITY Imaging whole brains with CLARITY and multiphoton microscopy. Image a whole brain without sectioning? Investigate neuronal circuits without reconstruction? Perform molecular phenotyping without destroying subcellular structures? Understanding the brain with molecular resolution and global scope has always been challenging. The novel CLARITY method, developed by the Deisseroth laboratory at Stanford University, USA, pushes the barrier of deep tissue imaging a big step ahead. https://www.leica-microsystems.com//science-lab/map-the-brain-with-clarity/ Tue, 11 Mar 2014 07:26:00 +0000 PhD Isabelle Köster https://www.leica-microsystems.com/6742 Laser Microdissection Comparison of the RNA Quality of Native Tissue Samples with Tissue Samples after UV Laser Microdissection Due to its instability, RNA is generally more difficult to work with than DNA. RNA does not have the stability of the DNA double helix. To obtain the best results when dealing with RNA it is essential to start out with high-quality material and to carry out particularly careful quality control before and after processing. The so-called RIN (RNA Integrity Number) is an indicator of the quality of RNA. On a scale of 1–10, a RIN value of 1 indicates that the RNA is completely degraded and a RIN value of 10 that the RNA is fully intact. The higher the RIN number, the better the RNA quality. Wherever possible, material with high RIN numbers should always be used. https://www.leica-microsystems.com//science-lab/comparison-of-the-rna-quality-of-native-tissue-samples-with-tissue-samples-after-uv-laser-microdissection/ Sun, 30 Sep 2012 22:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/11027 EM Sample Preparation Neuroscience Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron microscope (FIB/SEM). The samples are fixed with aldehydes, heavy metal stained using osmium tetroxide and uranyl acetate. They are then dehydrated with alcohol and infiltrated with resin, which is then hardened. https://www.leica-microsystems.com//science-lab/focussed-ion-beam-milling-and-scanning-electron-microscopy-of-brain-tissue/ Wed, 06 Jul 2011 16:17:00 +0000 https://www.leica-microsystems.com/9689 Neuroscience Confocal Microscopy Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further and currently there are many different ways to prepare organotypic cultures. The method presented here was adapted from the one described by Stoppini et al. for the preparation of the slices and from Gogolla et al. for the staining procedure. https://www.leica-microsystems.com//science-lab/organotypic-cerebellar-cultures-apoptotic-challenges-and-detection/ Tue, 17 May 2011 17:50:00 +0000