Leica Science Lab - Tag : Cancer Research https://www.leica-microsystems.com//science-lab/tag/tags/cancer-research/show/Tag/ Article tagged with Cancer Research en-US https://www.leica-microsystems.com/4421 Coherent Raman Scattering (CRS) Coherent Raman Scattering Microscopy Publication List CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their molecules. The two most important CRS techniques are Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). The biochemical image contrast of CRS is in many ways complementary to the molecular contrast obtained in fluorescence microscopy. A second crucial advantage of these methods is that they preserve the specimen/sample in a near pristine state. This reference list presents current and basic papers on CRS microscopy. https://www.leica-microsystems.com//science-lab/cars-publication-list/ Sun, 26 May 2019 22:00:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/3815 Laser Microdissection Neuroscience Forensics Laser Microdissection Publication List This monthly updated reference list demonstrates the major application fields for laser microdissection in life science research. https://www.leica-microsystems.com//science-lab/laser-microdissection-publication-list/ Sun, 28 Apr 2019 22:00:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/24929 Confocal Microscopy Visualize mechanical interactions of Cancer Cells Cells interact with their environment. Not only on the base of chemical signals, but also by sensing and modifying mechanical properties of the extracellular matrix. The research goal of Dr. Bo Sun’s group at Oregon State University is to understand such interactions. One of their tools is visualization of matrix properties with a TCS SPE confocal microscope. https://www.leica-microsystems.com//science-lab/visualize-mechanical-interactions-of-cancer-cells/ Tue, 16 Apr 2019 22:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/20306 Super-Resolution STED Nanoscopy at the forefront of cancer research Alison Dun is the postdoctoral facility manager for the Edinburgh Super-Resolution Imaging Consortium (ESRIC), Heriot-Watt University, Edinburgh, UK. She has used a large range of microscope techniques during her PhD work. In a video published by BBC News, Alison Dun explains how STED (stimulated emission depletion) microscopes help researchers in the fight against cancer by getting a better understanding of the processes that go wrong in the human body, thus giving hope for future treatments. https://www.leica-microsystems.com//science-lab/sted-nanoscopy-at-the-forefront-of-cancer-research/ Thu, 05 Jul 2018 22:00:00 +0000 PhD Alison Dun, Dr. Julia Roberti https://www.leica-microsystems.com/20478 Super-Resolution California NanoSystems Institute at UCLA Publications A list of the published scientific articles which include work done in the ALMS/MSI Facilities. https://www.leica-microsystems.com//science-lab/cnsi-publication-list/ Sun, 14 Jan 2018 23:00:00 +0000 https://www.leica-microsystems.com/19451 Live-Cell Imaging Fluorescence Microscopy Multiphoton Microscopy Five Questions Asked: Prof. Dr. Jacco van Rheenen speaks about the most important considerations when imaging deep into mouse tissue When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to explain the significance of the pinhole for those, who did not want to spend too much time to dig into theory and details of confocal microscopy but wanted to have an idea about the effect of the pinhole. https://www.leica-microsystems.com//science-lab/five-questions-asked-prof-dr-jacco-van-rheenen-speaks-about-the-most-important-considerations-when-imaging-deep-into-mouse-tissue/ Fri, 19 May 2017 05:47:00 +0000 PhD Jacco van Rheenen, Dr. Bernd Sägmüller https://www.leica-microsystems.com/19257 Stereo Microscopy Fluorescence Microscopy Work Efficiently in Developmental Biology and Medical Research with Stereo Microscopy: Rodent and Small Animal Surgery This report provides information which can help improve the routine work of scientists and technicians performing studies involving surgery on small animals and rodents, i.e. mice, rats, hamsters etc., for developmental biology or medical research. The aim is to help make the work steps efficient and cost-effective, where the employment of microscopes is necessary. It also gives useful hints and details on the various microscopes which can be used in a developmental biology or medical research laboratory where small animal or rodent surgery is exploited. https://www.leica-microsystems.com//science-lab/work-efficiently-in-developmental-biology-and-medical-research-with-stereo-microscopy-rodent-and-small-animal-surgery/ Mon, 27 Mar 2017 08:08:00 +0000 PhD James DeRose, PhD Heinrich Bürgers, MSc Ayyakkannu Ayyanan, MD Cathrin Brisken https://www.leica-microsystems.com/18172 Multiphoton Microscopy Fluorescence Microscopy Chronic Stress in Mice Remodels Lymph Vasculature to Promote Tumour Cell Dissemination Chronic stress induces signalling from the sympathetic nervous system (SNS) and drives cancer progression, although the pathways of tumour cell dissemination are unclear. Here we show that chronic stress restructures lymphatic networks within and around tumours to provide pathways for tumour cell escape. We show that VEGFC derived from tumour cells is required for stress to induce lymphatic remodelling and that this depends on COX2 inflammatory signalling from macrophages. Pharmacological inhibition of SNS signalling blocks the effect of chronic stress on lymphatic remodelling in vivo and reduces lymphatic metastasis in preclinical cancer models and in patients with breast cancer. https://www.leica-microsystems.com//science-lab/chronic-stress-in-mice-remodels-lymph-vasculature-to-promote-tumour-cell-dissemination/ Tue, 07 Feb 2017 09:52:00 +0000 https://www.leica-microsystems.com/18937 Super-Resolution Neuroscience Image Restoration and Deconvolution Quantitative Analysis of PPT1 Interactome in Human Neuroblastoma Cells Application example of HyVolution Super-Resolution - Mutations in the CLN1 gene that encodes Palmitoyl protein thioesterase 1 (PPT1) or CLN1, cause Infantile NCL (INCL, MIM#256730). PPT1 removes long fatty acid chains such as palmitate from modified cysteine residues of proteins. The data shown here result from isolated protein complexes from PPT1-expressing SH-SY5Y stable cells that were subjected to single step affinity purification coupled to mass spectrometry (AP-MS). Prior to the MS analysis, we utilised a modified filter-aided sample preparation (FASP) protocol. Based on label free quantitative analysis of the data by SAINT, 23 PPT1 interacting partners (IP) were identified. https://www.leica-microsystems.com//science-lab/quantitative-analysis-of-ppt1-interactome-in-human-neuroblastoma-cells/ Fri, 13 Jan 2017 16:23:00 +0000 https://www.leica-microsystems.com/18558 Laser Microdissection MicroRNA Expression Profiling to Identify and Validate Reference Genes for the Relative Quantification of microRNA in Rectal Cancer Application example for laser microdissection (LMD) - MicroRNAs (miRNAs) play important roles in regulating biological processes at the post-transcriptional level. Deregulation of miRNAs has been observed in cancer, and miRNAs are being investigated as potential biomarkers regarding diagnosis, prognosis and prediction in cancer management. Real-time quantitative polymerase chain reaction (RT-qPCR) is commonly used, when measuring miRNA expression. Appropriate normalisation of RT-qPCR data is important to ensure reliable results. The aim of the present study was to identify stably expressed miRNAs applicable as normaliser candidates in future studies of miRNA expression in rectal cancer. https://www.leica-microsystems.com//science-lab/microrna-expression-profiling-to-identify-and-validate-reference-genes-for-the-relative-quantification-of-microrna-in-rectal-cancer/ Wed, 07 Dec 2016 14:44:00 +0000 https://www.leica-microsystems.com/18900 Super-Resolution Confocal Microscopy Image Restoration and Deconvolution P53- and Mevalonate Pathway–Driven Malignancies Require Arf6 for Metastasis and Drug Resistance Application example of HvYolution Super-Resolution - Drug resistance, metastasis, and a mesenchymal transcriptional program are central features of aggressive breast tumors. The GTPase Arf6, often overexpressed in tumors, is critical to promote epithelial–mesenchymal transition and invasiveness. The metabolic mevalonate pathway (MVP) is associated with tumor invasiveness and known to prenylate proteins, but which prenylated proteins are critical for MVP-driven cancers is unknown. We show here that MVP requires the Arf6-dependent mesenchymal program. https://www.leica-microsystems.com//science-lab/p53-and-mevalonate-pathway-driven-malignancies-require-arf6-for-metastasis-and-drug-resistance/ Wed, 19 Oct 2016 16:27:00 +0000 https://www.leica-microsystems.com/18860 Webinar: Laser Microdissection in Cancer Research – Mutation Analysis Workflow with Pure Cancer Material Cancer can affect various organs and is caused by mutations of the DNA. A prerequisite, to explore and understand underlying gene-mutations involved in the development of a definite type of cancer, is the extraction of pure sample material, which is challenging. In this webinar, we will present how to extract 100% pure cancer tissue for DNA analysis with laser microdissection (LMD). Using tissue samples from human kidney cancer patients as an example, this webinar will provide an overview of the practical considerations when preparing a workflow to obtain highly pure material with the LMD microscope for further molecular analysis. https://www.leica-microsystems.com//science-lab/webinar-laser-microdissection-in-cancer-research-mutation-analysis-workflow-with-pure-cancer-material/ Tue, 04 Oct 2016 08:57:00 +0000 PhD Tamara Straube, Prof. Ralf Jacob https://www.leica-microsystems.com/18000 EM Sample Preparation Epoxy Resin Embedding of Animal and Human Tissues for Pathological Diagnosis and Research Application Note for Leica EM AMW - The tissues were fixed in the modified Karnovsky fixative generally by immersion overnight (at minimum 4h at room temperature). Then pieces of approx. 1mm3 were cut with a sharp razor blade and processed for embedding in the Leica EM AMW Microwave Tissue Processor. https://www.leica-microsystems.com//science-lab/epoxy-resin-embedding-of-animal-and-human-tissues-for-pathological-diagnosis-and-research/ Fri, 19 Aug 2016 08:34:00 +0000 Dr. Josef A. Schroeder https://www.leica-microsystems.com/18108 White Confocal Fluorescence Microscopy The Oncogenic Triangle of HMGA2, LIN28B and IGF2BP1 Antagonizes Tumor-Suppressive Actions of the let-7 Family The tumor-suppressive let-7 microRNA family targets various oncogene-encoding mRNAs. We identify the let-7 targets HMGA2, LIN28B and IGF2BP1 to form a let-7 antagonizing self-promoting oncogenic triangle. Surprisingly, 3′-end processing of IGF2BP1 mRNAs is unaltered in aggressive cancers and tumor-derived cells although IGF2BP1 synthesis was proposed to escape let-7 attack by APA-dependent (alternative polyadenylation) 3′ UTR shortening. https://www.leica-microsystems.com//science-lab/the-oncogenic-triangle-of-hmga2-lin28b-and-igf2bp1-antagonizes-tumor-suppressive-actions-of-the-let-7-family/ Fri, 17 Jun 2016 13:49:00 +0000 https://www.leica-microsystems.com/17939 Laser Microdissection Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a perfect tool for this purpose and how this was taught in the course of a workshop held in Brazil. With the Leica LMD system pure tumor material can be selected and dissected for downstream analysis to ensure 100% pure starting material without any risk of cross contamination with healthy cells. https://www.leica-microsystems.com//science-lab/workflows-protocols-laser-microdissection-for-pathology-and-cancer-research/ Wed, 04 May 2016 11:30:00 +0000 Dr. Falk Schlaudraff, Dr. Christoph Greb https://www.leica-microsystems.com/17437 Fluorescence Microscopy Stereo Microscopy Investigating the Influence of Hormone Signaling Pathways on Mammary Gland Development and the Onset of Breast Cancer Over their entire lifetime, 1 out of 8 women can suffer from breast cancer. The risk of developing breast cancer (mammary carcinogenesis) increases with a woman’s age and is related to her reproductive history. The chance of mammary carcinogenesis is less for women who give birth to a child before the age of 30. However, it is now known that the risk of breast cancer also can depend on the number of times a woman experiences elevated blood serum progesterone levels, in relation to the menstrual cycle, before her first pregnancy. https://www.leica-microsystems.com//science-lab/investigating-the-influence-of-hormone-signaling-pathways-on-mammary-gland-development-and-the-onset-of-breast-cancer/ Thu, 21 Jan 2016 11:12:00 +0000 MD Cathrin Brisken, PhD Renuga Devi Rajaram, PhD Duje Buric, MSc Ayyakkannu Ayyanan, PhD James DeRose https://www.leica-microsystems.com/16458 Light Sheet Microscopy Super-Resolution Confocal Microscopy "The Leica Digital Light Sheet Module – a Clever Example of Thinking Out of the Box" Bram van den Broek is a postdoctoral fellow at the Netherlands cancer institute in Amsterdam where he supports the advanced microscopy techniques in the laboratory of Kees Jalink. Working with Leica Microsystems as a collaboration partner for beta-testing of microscopes he enjoys very much. https://www.leica-microsystems.com//science-lab/the-leica-digital-light-sheet-module-a-clever-example-of-thinking-out-of-the-box/ Tue, 29 Sep 2015 14:39:00 +0000 Bram van den Broek, Dipl. oec.-troph. Anja Schué, PhD Isabelle Köster https://www.leica-microsystems.com/16097 Super-Resolution Localization of HDAC1 Using Super-Resolution STED Microscopy Here we show staining of HDAC1 in cancer tissue and epidermoid carcinoma cells. These results clearly show that the use of appropriate validated antibodies and STED microscopy are important tools to study subcellular structures beyond the diffraction limit correcting ill-defined images. This is critical in co-localization studies of proteins inside cells. https://www.leica-microsystems.com//science-lab/localization-of-hdac1-using-super-resolution-sted-microscopy/ Wed, 02 Sep 2015 15:57:00 +0000 PhD Karin Abarca Heidemann, PhD Sarah Crowe, MS, PhD Tobias Jacob, PhD Carl A. Ascoli https://www.leica-microsystems.com/14920 Multiphoton Microscopy CLEM Live-Cell Imaging Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points Cancer research unsing multiphoton microscopy and 3D electron microscopy. Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient events at high resolution. Performing correlative microscopy in complex and bulky samples such as an entire living organism is a time-consuming and error-prone task. https://www.leica-microsystems.com//science-lab/correlating-intravital-multi-photon-microscopy-to-3d-electron-microscopy-of-invading-tumor-cells-using-anatomical-reference-points/ Fri, 24 Apr 2015 13:06:00 +0000 https://www.leica-microsystems.com/15113 Live-Cell Imaging Fluorescence Microscopy Immunotherapy to Combat Cancer: "Sleeping Beauty" – DNA Plasmid-based Gene Transfer System to Modify T Cells Fighting cancer is a major goal of present-day medicine. So far mainly surgery, chemotherapy or radiation therapy are utilized to extinguish cancerous tissue, or at least set limits to it. Interestingly the human immune system has effective potential to fight cancer cells. Typically it reacts on parasitic, viral or bacterial infections. Thereby T-cells help to destroy infested cells after binding them via their specific antigen receptor. https://www.leica-microsystems.com//science-lab/immunotherapy-to-combat-cancer-sleeping-beauty-dna-plasmid-based-gene-transfer-system-to-modify-t-cells/ Wed, 28 Jan 2015 19:55:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/14774 Laser Microdissection Neuroscience Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and infiltrate brain parenchyma, they closely interact with a changing micro-environmental landscape that sustains tumor cell invasion. https://www.leica-microsystems.com//science-lab/identification-of-molecular-pathways-facilitating-glioma-cell-invasion-in-situ/ Mon, 22 Dec 2014 09:35:00 +0000 https://www.leica-microsystems.com/13879 Super-Resolution Video Interviews with Kees Jalink Kees Jalink's group at the Netherlands Cancer Institute in Amsterdam, The Netherlands, explores signal transduction pathways and cell adhesion processes in cancer cells. In his eyes especially the new three-dimensional nanoscopic view of the relevant structure of interest is an essential feature to get the full picture. https://www.leica-microsystems.com//science-lab/video-interviews-with-kees-jalink/ Fri, 29 Aug 2014 11:58:00 +0000 PhD Kees Jalink, MSc Leila Nahidiazar, Dipl. oec.-troph. Anja Schué, Dr. Christoph Greb https://www.leica-microsystems.com/14066 Multiphoton Microscopy Live-Cell Imaging The Environment Makes the Stem Cell A recent publication in Nature shows that all stem cells divide and compete for niche space by passively "kicking out" others so that eventually one stem cell takes over the whole niche. Jacco van Rheenen and Saskia Ellenbroek talk about a new method of intravital imaging, which allows following the fate of individual stem cells over time in vivo and explains the new paradigm for stem cell development in the intestinal stem cell niche. https://www.leica-microsystems.com//science-lab/the-environment-makes-the-stem-cell/ Mon, 04 Aug 2014 16:25:00 +0000 PhD Jacco van Rheenen, PhD Saskia I. J. Ellenbroek, PhD Isabelle Köster https://www.leica-microsystems.com/13913 Laser Microdissection Glucose-Regulated Protein 78 (Grp78) Confers Chemoresistance to Tumor Endothelial Cells under Acidic Stress This study was designed to investigate the activation of the unfolded protein response (UPR) in tumor associated endothelial cells (TECs) and its association with chemoresistance during acidic pH stress. https://www.leica-microsystems.com//science-lab/glucose-regulated-protein-78-grp78-confers-chemoresistance-to-tumor-endothelial-cells-under-acidic-stress/ Mon, 21 Jul 2014 17:16:00 +0000 https://www.leica-microsystems.com/12452 Neuroscience Live-Cell Imaging The 50 Most Influential Scientists in the World Today From neuroscience, biotechnology and digital media to sustainable energy and cloud computing, almost everything today is somehow affected – and sometimes entirely reshaped – by scientific and technological advances. https://www.leica-microsystems.com//science-lab/good-to-know/the-50-most-influential-scientists-in-the-world-today/ Mon, 17 Feb 2014 19:03:00 +0000 https://www.leica-microsystems.com/10286 Live-Cell Imaging Multiphoton Microscopy Direct In Vivo Evidence for Tumor Propagation by Glioblastoma Cancer Stem Cells Cancer research using multiphoton microscopy. High-grade gliomas (World Health Organization grade III anaplastic astrocytoma and grade IV glioblastoma multiforme), the most prevalent primary malignant brain tumors, display a cellular hierarchy with self-renewing, tumorigenic cancer stem cells (CSCs) at the apex. While the CSC hypothesis has been an attractive model to describe many aspects of tumor behavior, it remains controversial due to unresolved issues including the use of ex vivo analyses with differential growth conditions. https://www.leica-microsystems.com//science-lab/direct-in-vivo-evidence-for-tumor-propagation-by-glioblastoma-cancer-stem-cells/ Fri, 12 Jul 2013 13:21:00 +0000 https://www.leica-microsystems.com/7275 Laser Microdissection Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome" The physiological process of aging is an elementary part of life. The processes responsible for the aging process and the effect they have is one of the key focuses of biomedical research. Dr. Yvonne Begus-Nahrmann researches molecular mechanisms in stem cells and their role in aging and tumor development at the Göttingen University Medical Center. Signal proteins such as p53 and p21 appear to play an important role in this context. To investigate these proteins in specific cell types, the cells are isolated from organ tissue with the aid of laser microdissection. https://www.leica-microsystems.com//science-lab/stem-cells-in-the-aging-process-the-signal-protein-p53-as-guardian-of-the-genome/ Thu, 08 Nov 2012 23:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille, Dr. Yvonne Begus-Nahrmann https://www.leica-microsystems.com/3835 Laser Microdissection Applications of Laser Microdissection Laser microdissection and laser micromanipulation are suitable for gaining a differentiated insight into the function of genes and proteins, and are used for a wide range of applications in neurobiology and immunology as well as in the developmental and cell biology of animal and plant organisms. https://www.leica-microsystems.com//science-lab/applications-of-laser-microdissection/ Sun, 26 Jun 2011 22:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/3862 Laser Microdissection Neuroscience Users Report on the Relevance of Laser Microdissection for Their Research Results Laser dissection is used in a large number of research fields, e.g. neurology, cancer research, plant analysis. Here, user report on the research results they have attained by using laser microdissection. https://www.leica-microsystems.com//science-lab/users-report-on-the-relevance-of-laser-microdissection-for-their-research-results/ Tue, 12 Apr 2011 22:00:00 +0000 Kerstin Pingel https://www.leica-microsystems.com/2561 Multiphoton Microscopy Live-Cell Imaging Confocal Microscopy Stem Cell Biology in Cancer Research The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence microscopy and confocal microscopy invaluable allies in the study of stem cells. https://www.leica-microsystems.com//science-lab/stem-cell-biology-in-cancer-research/ Thu, 22 Apr 2010 22:00:00 +0000 Laura Furia, Angelo Cicalese, Ilaria Rancati, Isabella Ponzanelli, Paolo Bianchini, Prof. Alberto Diaspro, Mario Faretta https://www.leica-microsystems.com/4290 Multiphoton Microscopy Confocal Microscopy Quantitative Fluorescence Image Restoration and Deconvolution From Molecules to Tissues Cancer research using confocal and multiphoton microscopy. Sequencing of the human genome stimulated a radical change in the approach to biomedical research. The comprehension of the mechanisms regulating life gained a scale-up in throughput to speed up the retrieval of data for a global vision of a system of incomparable complexity. https://www.leica-microsystems.com//science-lab/from-molecules-to-tissues/ Thu, 21 Aug 2008 22:00:00 +0000 Mario Faretta, Prof. Alberto Diaspro, Sara Barozzi, Massimiliano Garrè, Ivan Muradore, Andrea Palamidessi, Dario Parazzoli, Simona Ronzoni, Pietro Transidico, Davide Mazza, Ph.D. Illaria Testa