Leica Science Lab - Tag : Cell Culture https://www.leica-microsystems.com//science-lab/tag/tags/cell-culture/show/Tag/ Article tagged with Cell Culture en-US https://www.leica-microsystems.com/24755 Live-Cell Imaging How can PAULA help streamline the workflow for my wound healing assay? The wound healing assay, often used as a synonym for scratch assay or migration assay, is an important tool for the evaluation and measurement of cell migration, whether single cells or cell populations. Cell migration is tightly controlled during ontogenetic processes and at times can get out of control, e.g., during cancer development and progression. https://www.leica-microsystems.com//science-lab/how-can-paula-help-streamline-the-workflow-for-my-wound-healing-assay/ Mon, 18 Mar 2019 23:00:00 +0000 Dr. Karin Schwab https://www.leica-microsystems.com/24680 Live-Cell Imaging Confluency Check with PAULA Cell Imager Many cell-based experiments require cells in a certain state. This can include their morphology,  fluorescent protein expression, and the confluency of the cell layer. Researchers check their cells regularly to determine these attributes. Confluency estimation can be very difficult! This guess work is biased by every individual researcher, and can result in lab error.  https://www.leica-microsystems.com//science-lab/confluency-check-with-paula-cell-imager/ Wed, 20 Feb 2019 23:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/19686 Live-Cell Imaging Introduction to Mammalian Cell Culture Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research would be unthinkable. This article gives an overview of mammalian cell culture systems. Mainly, they can be categorized according to their morphology, as well as cell type and organization. Moreover, you can find basic information about the correct growth conditions and what kind of microscope you need to watch your cells. https://www.leica-microsystems.com//science-lab/introduction-to-mammalian-cell-culture/ Thu, 10 Aug 2017 10:54:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/18915 Super-Resolution Visualizing Tropoelastin in a Long-Term Human Elastic Fibre Cell Culture Model Elastin is an essential protein found in a variety of tissues where resilience and flexibility are needed, such as the skin and the heart. When aiming to engineer suitable implants, elastic fibres are needed to allow adequate tissue renewal. However, the visualization of human elastogenesis remains in the dark. To date, the visualization of human tropoelastin (TE) production in a human cell context and its fibre assembly under live cell conditions has not been achieved. Here, we present a long-term cell culture model of human dermal fibroblasts expressing fluorescence-labelled human TE. We employed a lentiviral system to stably overexpress Citrine-labelled TE to build a fluorescent fibre network. Using immunofluorescence, we confirmed the functionality of the Citrine-tagged TE. Furthermore, we visualized the fibre assembly over the course of several days using confocal microscopy. Applying super resolution microscopy, we were able to investigate the inner structure of the elastin–fibrillin-1 fibre network. https://www.leica-microsystems.com//science-lab/visualizing-tropoelastin-in-a-long-term-human-elastic-fibre-cell-culture-model/ Mon, 24 Oct 2016 10:10:00 +0000 https://www.leica-microsystems.com/17667 Live-Cell Imaging Widefield Microscopy How to do a Proper Cell Culture Quick Check In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth must be monitored at regular intervals. This article describes a typical workflow for subculturing an adherent cell line with detailed illustrations of all of the necessary steps. https://www.leica-microsystems.com//science-lab/how-to-do-a-proper-cell-culture-quick-check/ Thu, 24 Mar 2016 09:18:00 +0000 PhD Tamara Straube, Claudia Müller https://www.leica-microsystems.com/14978 Super-Resolution Confocal Microscopy Detailed Morphological Characterisation of Hendra Virus Infection of Different Cell Types Using Super-Resolution and Conventional Imaging Hendra virus (HeV) is a pleomorphic virus belonging to the Paramyxovirus family. Our long-term aim is to understand the process of assembly of HeV virions. As a first step, we sought to determine the most appropriate cell culture system with which to study this process, and then to use this model to define the morphology of the virus and identify the site of assembly by imaging key virus encoded proteins in infected cells. https://www.leica-microsystems.com//science-lab/detailed-morphological-characterisation-of-hendra-virus-infection-of-different-cell-types-using-super-resolution-and-conventional-imaging/ Wed, 13 May 2015 20:09:00 +0000 https://www.leica-microsystems.com/5738 EM Sample Preparation Dry Ultrathin Sectioning Combined With High Pressure Freezing We have used cultured UMR106-01 osteoblastic cells to investigate the process of bone mineralization. UMR106-01 cells as well as primary calvarial bone cells assembly spherical extracellular supramolecular protein-lipid complexes, termed biomineralization foci (BMF), in which the first crystals of hydroxyapatite mineral are deposited (Midura et al., 2004; Wang et al., 2004). A major difference between these culture models is the speed with which mineralization occurs, ranging from 12–16 days after plating for primary osteoblastic cells to 88 h for UMR106-01 cells. https://www.leica-microsystems.com//science-lab/dry-ultrathin-sectioning-combined-with-high-pressure-freezing/ Mon, 10 Dec 2012 23:00:00 +0000 Ph.D. Jeff P. Gorski, M.Sc. Nichole T. Huffman, Thérèse Hillman-Marti, Daniel Studer https://www.leica-microsystems.com/5275 White Confocal Confocal Microscopy How to Study Protein Recruitment to DNA Lesions by a Combination of UV Laser and White Light Laser Understanding how DNA lesions are optimally repaired is of functional significance, especially from the view of genome karyotype stability. https://www.leica-microsystems.com//science-lab/how-to-study-protein-recruitment-to-dna-lesions-by-a-combination-of-uv-laser-and-white-light-laser/ Wed, 22 Feb 2012 23:00:00 +0000 Assoc. Prof. Dr. Eva Bártová, Assoc. Prof. Dr. Stanislav Kozubek, Ph.D. Gabriela Sustáčková https://www.leica-microsystems.com/4392 Laser Microdissection Cell Cultures and Laser Microdissection Many of the discoveries that are now being made in cell division and differentiation, the relationships between single cells and cell organelles, treatments of cells with pharmaceutic substances, etc. would not be possible without live cell cultures. Allowing morphological and biochemical observations of single cells under different experimental conditions, they provide a unique source of information. https://www.leica-microsystems.com//science-lab/cell-cultures-and-laser-microdissection/ Fri, 07 Oct 2011 09:15:57 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/9689 Neuroscience Confocal Microscopy Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further and currently there are many different ways to prepare organotypic cultures. The method presented here was adapted from the one described by Stoppini et al. for the preparation of the slices and from Gogolla et al. for the staining procedure. https://www.leica-microsystems.com//science-lab/organotypic-cerebellar-cultures-apoptotic-challenges-and-detection/ Tue, 17 May 2011 17:50:00 +0000