Leica Science Lab - Tag : 256 https://www.leica-microsystems.com//science-lab/tag/tags/cemovis/show/Tag/ Article tagged with 256 en-US https://www.leica-microsystems.com/18295 EM Sample Preparation Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast Application Note for Leica EM HPM100 - The sections are of yeast frozen with a Leica HPM100 high pressure freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer so that a final MES concentration of 50mM and a dextran concentration of 20% was achieved. https://www.leica-microsystems.com//science-lab/cryo-electron-microscopy-of-vitreous-sections-cemovis-of-yeast/ Fri, 12 Aug 2016 14:51:00 +0000 Dr. Jonathan O'Driscoll, Dr. Daniel Kofi Clare, Prof. Helen Saibil https://www.leica-microsystems.com/14887 EM Sample Preparation Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing High pressure freezing (HPF) is a cryo-fixation method primarily for biological samples, but also for a variety of non-biological materials. It is a technique that yields optimal preservation in many cell types and tissues or in organic and inorganic composites. Most commonly, the high pressure frozen samples are analyzed further with light or electron microscopy after appropriate processing. https://www.leica-microsystems.com//science-lab/video-tutorials-filling-and-assembling-of-different-carriers-for-high-pressure-freezing/ Fri, 05 Dec 2014 08:45:00 +0000 Dr. Cveta Tomova https://www.leica-microsystems.com/10060 EM Sample Preparation CLEM Brief Introduction to High-Pressure Freezing Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant structural alterations is by cryo-fixation. There are currently two common methods employed; plunge freezing and high pressure freezing. https://www.leica-microsystems.com//science-lab/brief-introduction-to-high-pressure-freezing/ Wed, 12 Jun 2013 17:59:00 +0000 Dr. Cveta Tomova