Leica Science Lab - Tag : Cryo Electron Microscopy https://www.leica-microsystems.com//science-lab/tag/tags/cryo-electron-microscopy/show/Tag/ Article tagged with Cryo Electron Microscopy en-US https://www.leica-microsystems.com/27911 CLEM Improve Cryo Electron Tomography Workflow Leica Microsystems and Thermo Fisher Scientific have collaborated to create a fully integrated cryo-tomography workflow that responds to these research needs: Reveal cellular mechanisms at subnanometer resolution in 3D applying the fully integrated cryo electron tomography workflow https://www.leica-microsystems.com//science-lab/improve-cryo-electron-tomography-workflow/ Tue, 03 Sep 2019 09:50:00 +0000 https://www.leica-microsystems.com/24702 EM Sample Preparation Integrated Cryo-tomography Workflow Analyzing purified protein structures in a cellular context is key to understanding how proteins and protein complexes function. Thermo Fisher Scientific and Leica Microsystems developed the integrated cryo-tomography workflow to enable high resolution structural analysis, done in a controlled and effective way, of proteins in their native environments. In this webinar, the speakers will explain the unique features of the hardware and software integration and guide you step-by-step through the workflow. https://www.leica-microsystems.com//science-lab/changing-the-way-we-look-at-the-inner-workings-of-cells/ Sun, 03 Mar 2019 23:00:00 +0000 https://www.leica-microsystems.com/18008 CLEM Fluorescence Microscopy EM Sample Preparation Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy as a Straightforward Tool to Study Host-Pathogen Interactions Correlative light and electron microscopy is an imaging technique that enables identification and targeting of fluorescently tagged structures with subsequent imaging at near-to-nanometer resolution. We established a novel correlative cryo-fluorescence microscopy and cryo-scanning electron microscopy workflow, which enables imaging of the studied object of interest very close to its natural state, devoid of artifacts caused for instance by slow chemical fixation. This system was tested by investigating the interaction of the zoonotic bacterium Borrelia burgdorferi with two mammalian cell lines of neural origin in order to broaden our knowledge about the cell-association mechanisms that precedes the entry of the bacteria into the cell. https://www.leica-microsystems.com//science-lab/correlative-cryo-fluorescence-and-cryo-scanning-electron-microscopy-as-a-straightforward-tool-to-study-host-pathogen-interactions/ Tue, 13 Dec 2016 07:58:00 +0000 Martin Strnad https://www.leica-microsystems.com/18295 EM Sample Preparation Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast Application Note for Leica EM HPM100 - The sections are of yeast frozen with a Leica HPM100 high pressure freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer so that a final MES concentration of 50mM and a dextran concentration of 20% was achieved. https://www.leica-microsystems.com//science-lab/cryo-electron-microscopy-of-vitreous-sections-cemovis-of-yeast/ Fri, 12 Aug 2016 14:51:00 +0000 Dr. Jonathan O'Driscoll, Dr. Daniel Kofi Clare, Prof. Helen Saibil https://www.leica-microsystems.com/17382 CLEM Imaging of Host Cell-bacteria Interactions using Correlative Microscopy under Cryo-conditions Pathogenic bacteria have developed intriguing strategies to establish and promote infections in their respective hosts. Most bacterial pathogens initiate infectious diseases by adhering to host cells surface. Knowledge of the interplay between the pathogenic organism and the host cells can provide fundamental new insight into the underlying mechanisms of the infectious process and therefore of the disease. Various microscopy techniques have proven to be critical tools to study these events. https://www.leica-microsystems.com//science-lab/imaging-of-host-cell-bacteria-interactions-using-correlative-microscopy-under-cryo-conditions/ Tue, 01 Mar 2016 11:34:00 +0000 Martin Strnad https://www.leica-microsystems.com/16589 EM Sample Preparation Cryo-electron Microscopy of Tubular Arrays of HIV-1 Gag Resolves Structures Essential for Immature Virus Assembly HIV-1 undergoes a two-step assembly process. First, an immature noninfectious particle is assembled, which leaves the infected cell. Second, the structural protein, Gag, is cleaved in the virus by the viral protease, and this leads to formation of the infectious virus. We have assembled part of HIV-1 Gag in vitro to form immature virus-like tubular protein arrays, and have solved a subnanometer-resolution structure of these arrays by using cryo-EM and tomography. This structure reveals interactions of the C-terminal capsid domain of Gag that are critical for HIV-1 assembly. https://www.leica-microsystems.com//science-lab/cryo-electron-microscopy-of-tubular-arrays-of-hiv-1-gag-resolves-structures-essential-for-immature-virus-assembly/ Tue, 09 Feb 2016 12:27:00 +0000 https://www.leica-microsystems.com/15509 CLEM Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer superior structural preservation, performing in-resin super-resolution, however, remains a challenge. https://www.leica-microsystems.com//science-lab/correlative-in-resin-super-resolution-and-electron-microscopy-using-standard-fluorescent-proteins/ Thu, 04 Jun 2015 10:28:00 +0000 https://www.leica-microsystems.com/15106 EM Sample Preparation Fluorescence Microscopy Cryo CLEM – the Combination of Cryo Fluorescence Microscopy with Cryo Electron Microscopy Many biological insights can be obtained by combining the power of Fluorescence Microscopy (FM) with that of Electron Microscopy (EM) to study the same sample – this is called Correlative Light and Electron Microscopy (CLEM). In FM, specific proteins can be labelled and identified, and their dynamics and interactions can be visualized in fixed or living cells. https://www.leica-microsystems.com//science-lab/cryo-clem-the-combination-of-cryo-fluorescence-microscopy-with-cryo-electron-microscopy/ Wed, 18 Mar 2015 08:17:00 +0000 PhD Martin Schorb, PhD John A. G. Briggs