Leica Science Lab - Tag : Cytoskeleton https://www.leica-microsystems.com//science-lab/tag/tags/cytoskeleton/show/Tag/ Article tagged with Cytoskeleton en-US https://www.leica-microsystems.com/26320 Live-Cell Imaging TIRF Microscopy Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the environment. Still, they have a distinct shape, can easily alter their shape, and even change their shape to move around. This is possible thanks to a flexible “inner wall” that is composed of the actin cortex and the cytoskeleton. https://www.leica-microsystems.com//science-lab/where-to-go-cellular-migration-requires-coordinated-transitions-of-actin-cortex/ Mon, 19 Aug 2019 13:27:00 +0000 PhD Olga Davydenko, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/26318 Neuroscience Confocal Microscopy Cellular Motility: Microtubules, Motor Proteins and Tau-Proteins Cellular motility is based on motor-proteins that can bind to filamentous scaffold proteins and – under consumption of ATP – can “crawl” on these filaments. This note is about proteins connected to microtubules, one of the filamentous structures that compose the cytoskeleton. Microtubules are hollow tubes of ca 25nm, composed of tubulin-heterodimers. The proteins are polymerized in a directed fashion, allowing to differentiate a plus-end and a minus-end of the fiber. Another important scaffold component that is also involved in movements, are actin fibers that cooperate with myosin as a motor-protein. The best known movement involving the actin-myosin system is muscular contraction. https://www.leica-microsystems.com//science-lab/cellular-motility-microtubules-motor-proteins-and-tau-proteins/ Mon, 12 Aug 2019 12:34:00 +0000 PhD Jen-Yi Lee, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/18921 Super-Resolution The Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy Hemidesmosomes have been extensively studied by immunofluorescence microscopy, but due to its limited resolution, their precise organization remained poorly understood. We studied hemidesmosome organization in cultured keratinocytes by 2- and 3-color super-resolution microscopy. We observed that in the cell periphery, nascent hemidesmosomes are associated with individual keratin filaments and that β4 is distributed along rather than under keratin filaments. By applying innovative methods to quantify molecular distances, we demonstrate that the hemidesmosomal plaque protein plectin interacts simultaneously and asymmetrically with β4 and keratin. https://www.leica-microsystems.com//science-lab/the-molecular-architecture-of-hemidesmosomes-as-revealed-by-super-resolution-microscopy/ Fri, 10 Feb 2017 09:11:00 +0000 MSc Leila Nahidiazar https://www.leica-microsystems.com/18098 Super-Resolution Actin-Dependent Vacuolar Occupancy of the Cell Determines Auxin-Induced Growth Repression The cytoskeleton is an early attribute of cellular life, and its main components are composed of conserved proteins. The actin cytoskeleton has a direct impact on the control of cell size in animal cells, but its mechanistic contribution to cellular growth in plants remains largely elusive. Here, we reveal a role of actin in regulating cell size in plants. The actin cytoskeleton shows proximity to vacuoles, and the phytohormone auxin not only controls the organization of actin filaments but also impacts vacuolar morphogenesis in an actin-dependent manner. https://www.leica-microsystems.com//science-lab/actin-dependent-vacuolar-occupancy-of-the-cell-determines-auxin-induced-growth-repression/ Fri, 03 Feb 2017 08:14:00 +0000 https://www.leica-microsystems.com/18806 Super-Resolution Practical Guide for Excellent GSDIM Super-Resolution Images Do you know that most protists and bacteria lack in one feature that each of our body cell has? Our cells are touch and communicate with one another. They send and receive a variety of signals that coordinate their behavior to act together as a functional multicellular organism. Exploring the way of cellular communication and the ways how the cell surface interacts to organize tissues and body structures is of great interest. Kees Jalink and his team of scientists at the Netherlands Cancer Institute (NKI) in Amsterdam obtained new scientific insights into the molecular architecture of hemidesmosomes, cytoskeletal components, cell surface receptors and vesicular proteins with the help of Ground-State-Depletion (GSD)/ dSTORM microscopy. In this interview, Kees Jalink comments on their developments in imaging chambers, buffer conditions and image analysis to get the perfect super resolution image. https://www.leica-microsystems.com//science-lab/practical-guide-for-excellent-gsdim-super-resolution-images/ Wed, 26 Oct 2016 06:58:00 +0000 PhD Kees Jalink, PhD Tamara Straube, MSc Leila Nahidiazar, MSc Daniela Leyton Puig https://www.leica-microsystems.com/18919 Super-Resolution Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the mechanism of initiation of lamellipodia and ruffles remains poorly understood. Here, we show that mDia1 acts in concert with the Arp2/3 complex to promote initiation of lamellipodia and ruffles. https://www.leica-microsystems.com//science-lab/initiation-of-lamellipodia-and-ruffles-involves-cooperation-between-mdia1-and-the-arp23-complex/ Mon, 24 Oct 2016 11:47:00 +0000 https://www.leica-microsystems.com/18102 Super-Resolution The Actin Cytoskeleton Modulates the Activation of iNKT Cells by Segregating CD1d Nanoclusters on Antigen-Presenting Cells The ability of invariant natural killer T (iNKT) cells to recognize endogenous antigens represents a distinct immune recognition strategy, which underscores the constitutive memory phenotype of iNKT cells and their activation during inflammatory conditions. By using superresolution microscopy, we show that CD1d molecules form nanoclusters at the cell surface of APCs, and their size and density are constrained by the actin cytoskeleton. https://www.leica-microsystems.com//science-lab/the-actin-cytoskeleton-modulates-the-activation-of-inkt-cells-by-segregating-cd1d-nanoclusters-on-antigen-presenting-cells/ Wed, 10 Aug 2016 13:28:00 +0000 https://www.leica-microsystems.com/18917 Super-Resolution TIRF Microscopy Co-Orientation: Quantifying Simultaneous Co-Localization and Orientational Alignment of Filaments in Light Microscopy Co-localization analysis is a widely used tool to seek evidence for functional interactions between molecules in different color channels in microscopic images. Here we extend the basic co-localization analysis by including the orientations of the structures on which the molecules reside. We refer to the combination of co-localization of molecules and orientational alignment of the structures on which they reside as co-orientation. Because the orientation varies with the length scale at which it is evaluated, we consider this scale as a separate informative dimension in the analysis. Additionally we introduce a data driven method for testing the statistical significance of the co-orientation and provide a method for visualizing the local co-orientation strength in images. We demonstrate our methods on simulated localization microscopy data of filamentous structures, as well as experimental images of similar structures acquired with localization microscopy in different color channels. https://www.leica-microsystems.com//science-lab/co-orientation-quantifying-simultaneous-co-localization-and-orientational-alignment-of-filaments-in-light-microscopy/ Wed, 16 Sep 2015 15:20:00 +0000 Robert Nieuwenhuizen, MSc Leila Nahidiazar https://www.leica-microsystems.com/16174 Super-Resolution Quantitative Fluorescence Live-Cell Imaging Cortical Actin Networks Induce Spatio-temporal Confinement of Phospholipids in the Plasma Membrane – A Minimally Invasive Investigation by STED-FCS Important discoveries in the last decades have changed our view of the plasma membrane organisation. Specifically, the cortical cytoskeleton has emerged as a key modulator of the lateral diffusion of membrane proteins. Cytoskeleton-dependent compartmentalised lipid diffusion has been proposed, but this concept remains controversial because this phenomenon has thus far only been observed with artefact-prone probes in combination with a single technique: single particle tracking. https://www.leica-microsystems.com//science-lab/cortical-actin-networks-induce-spatio-temporal-confinement-of-phospholipids-in-the-plasma-membrane-a-minimally-invasive-investigation-by-sted-fcs/ Fri, 24 Jul 2015 13:02:00 +0000 Débora Machado Andrade https://www.leica-microsystems.com/15104 EM Sample Preparation Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications A well established usage case for cryo-TEM is three-dimensional reconstruction of isolated macromolecules, virus particles, or filaments. On one hand, these approaches are based on averaging of repetitive structures – either due to numerous identical molecules, repetitive patterns on a filament, or symmetries, to reduce the noise inherent to cryo-TEM. https://www.leica-microsystems.com//science-lab/immersion-freezing-for-cryo-transmission-electron-microscopy-applications/ Mon, 09 Feb 2015 14:51:00 +0000 Dr. Guenter Resch https://www.leica-microsystems.com/15219 Confocal Microscopy "Leica is always flexible and dynamic" - Interview with Audrey Salles, Pasteur Institute, Paris Audrey Salles is a specialist for confocal and super-resolution microscopy at Pasteur Institute, Imagopole, PFID, Paris, France. Her research interests are cytokine signaling and skeleton organization of human TCD4-cells. https://www.leica-microsystems.com//science-lab/leica-is-always-flexible-and-dynamic-interview-with-audrey-salles-pasteur-institute-paris/ Mon, 12 Jan 2015 11:10:00 +0000 Audrey Salles, PhD Isabelle Köster, Dipl. oec.-troph. Anja Schué https://www.leica-microsystems.com/14656 Confocal Microscopy Super-Resolution CLEM Live-Cell Imaging Quantitative Fluorescence ICln: A New Regulator of Non-Erythroid 4.1R Localisation and Function To optimise the efficiency of cell machinery, cells can use the same protein (often called a hub protein) to participate in different cell functions by simply changing its target molecules. There are large data sets describing protein-protein interactions ("interactome") but they frequently fail to consider the functional significance of the interactions themselves. https://www.leica-microsystems.com//science-lab/icln-a-new-regulator-of-non-erythroid-41r-localisation-and-function/ Mon, 03 Nov 2014 18:02:00 +0000 https://www.leica-microsystems.com/13879 Super-Resolution Video Interviews with Kees Jalink Kees Jalink's group at the Netherlands Cancer Institute in Amsterdam, The Netherlands, explores signal transduction pathways and cell adhesion processes in cancer cells. In his eyes especially the new three-dimensional nanoscopic view of the relevant structure of interest is an essential feature to get the full picture. https://www.leica-microsystems.com//science-lab/video-interviews-with-kees-jalink/ Fri, 29 Aug 2014 11:58:00 +0000 PhD Kees Jalink, MSc Leila Nahidiazar, Dipl. oec.-troph. Anja Schué, Dr. Christoph Greb https://www.leica-microsystems.com/14017 Super-Resolution Image Restoration and Deconvolution Abstracts of the 4th European Super-Resolution User-Club Meeting The 4th Super-Resolution User Club Meeting was held in collaboration with Christian Eggeling and the Weatherall Institute of Molecular Medicine in Oxford, UK. Here we present the abstracts of the talks and interviews with participants. https://www.leica-microsystems.com//science-lab/abstracts-of-the-4th-european-super-resolution-user-club-meeting/ Fri, 08 Aug 2014 08:09:00 +0000 Prof. Christian Eggeling, Dr. Timo Zimmermann, Dr. Katrin Willig, PhD Kees Jalink, Dr. Marko Lampe, Prof. Mark Neil, Dr. Sergi Padilla-Parra, MSc Leila Nahidiazar, Ph.D. Giuseppe Vicidomini, Ph.D. Marc van Zandvoort https://www.leica-microsystems.com/13128 Super-Resolution Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton. https://www.leica-microsystems.com//science-lab/visualization-of-the-immunological-synapse-by-dual-color-time-gated-stimulated-emission-depletion-sted-nanoscopy/ Fri, 28 Mar 2014 16:34:00 +0000 Ph.D. Emily M. Mace, Ph.D., MD Jordan S. Orange