Leica Science Lab - Tag : Dark States https://www.leica-microsystems.com//science-lab/tag/tags/dark-states/show/Tag/ Article tagged with Dark States en-US https://www.leica-microsystems.com/6497 Widefield Microscopy Super-Resolution Widefield Super-Resolution with GSDIM Great advancements in biology have been possible by using fluorescence microscopy. So far, the resolution of the images was limited due to physical constraints. In the past couple of years, new methods evolved circumventing these limitations and bringing fluorescence microscopy to a new level of resolution, boosting the possibilities in science with fluorescence microscopes. https://www.leica-microsystems.com//science-lab/widefield-super-resolution-with-gsdim/ Wed, 11 Jul 2012 22:00:00 +0000 Dr. Marko Lampe https://www.leica-microsystems.com/4621 Super-Resolution Widefield Microscopy How Widefield Super-Resolution GSDIM Images are Created The localization microscopy technique GSDIM is a proven technology to achieve super-resolution images with a resolution of up to 20 nm. In the following tutorial we will describe the basic principles and features of GSDIM. https://www.leica-microsystems.com//science-lab/how-widefield-super-resolution-gsdim-images-are-created/ Mon, 31 Oct 2011 17:17:00 +0000 Dr. Marko Lampe https://www.leica-microsystems.com/4600 Super-Resolution Step by Step Guide to the Molecular Basics of GSDIM Microscopy Ground state depletion microscopy followed by individual molecule return (GSDIM) is a super-resolution technique based on single molecule localization (Localization Microscopy). To localize single molecules and create a high resolution image the ensemble of overlapping fluorophores (in a diffraction-limited setup) has to be broken up. Individual fluorophores must be temporally "separated" to allow high precision detection of single molecules. This tutorial will explain the molecular basics of GSDIM. https://www.leica-microsystems.com//science-lab/step-by-step-guide-to-the-molecular-basics-of-gsdim-microscopy/ Fri, 28 Oct 2011 16:17:00 +0000 Dr. Marko Lampe https://www.leica-microsystems.com/4438 Super-Resolution Super-Resolution GSDIM Microscopy The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within the cell. There is now a first commercial system (the Leica SR GSD) on the market that is helping to make the GSDIM technique available to a wider group of users in research labs and imaging centers. https://www.leica-microsystems.com//science-lab/super-resolution-gsdim-microscopy/ Thu, 20 Oct 2011 22:00:00 +0000 PhD Tamara Straube, Alexandra Elli, Prof. Ralf Jacob https://www.leica-microsystems.com/4126 Confocal Microscopy Fluorescence Microscopy Brighter Fluorescence by Resonant Scanning Fast True Confocal Scanning reduces photobleaching and increases the fluorescence yield at identical acquisition times. The long-lasting triplet state (or any other “dark state”) is less populated when the illumination is applied in shorter pulses at the same intensity. Consequently, more fluorochromes are available for the fluorescence process (brighter images) and fewer fluorochromes disintegrate from triplet states or excited triplet states (less bleaching). https://www.leica-microsystems.com//science-lab/brighter-fluorescence-by-resonant-scanning/ Wed, 03 Aug 2011 08:42:54 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/4186 Basics in Microscopy Fluorescence Microscopy Photo Effects of Light Molecules and atoms can exist in different quantum states. These states are dedicated to different energy levels; the quantum state with the lowest energy is called the ground state. Every state of greater energy is an excited state of the quantum mechanical system. Electrons can be excited by an exogenous energy source and switch to a higher energy level, changing the quantum state of the molecule or atom. The electrons are often referred to as being brought to a state of higher energy. https://www.leica-microsystems.com//science-lab/photo-effects-of-light/ Mon, 30 May 2011 15:13:00 +0000 Wymke Ockenga