Leica Science Lab - Tag : Dye https://www.leica-microsystems.com//science-lab/tag/tags/dye/show/Tag/ Article tagged with Dye en-US https://www.leica-microsystems.com/19429 Fluorescence Microscopy What is Photomanipulation? The term photomanipulation describes a wide range of techniques that enable the microscopist the transition from passive observer to instigator of events by offering a way of interacting with their sample via targeted illumination. Typically researchers are trying to observe specific processes of interest in order to understand the underlying biological process. Microscopists are often forced to hunt through large populations of cells or acquire hours of time laps footage before they’re able to observe events of interest and in many cases it’s simply not possible to observe certain processes using conventional microscopy techniques alone. Photomanipulation tools enable the microscopist to initiate biological events, precisely adjusting sample labeling, biological activity, local chemical environments and in some instances physically destroy parts of their specimen. https://www.leica-microsystems.com//science-lab/what-is-photomanipulation/ Mon, 15 May 2017 07:59:00 +0000 Dr. Peter Laskey, Dr. Christoph Greb, Dr. Oliver Schlicker https://www.leica-microsystems.com/6395 Fluorescence Microscopy Photoactivatable, photoconvertible, and photoswitchable Fluorescent Proteins Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits. Watching dedicated, spatially limited protein populations of a certain protein of interest is impossible with common FPs, since they are expressed throughout the entire cell. At this point photoactivatable, photoconvertible and photoswitchable fluorescent proteins enter the stage. The members of this fluorescence toolkit can be activated from a non-fluorescent state, they can change their emission spectrum, or they are even able to be reversibly switched "on and off". With the help of these “optical highlighters”, researchers can track a distinct protein population over time by activating respectively converting their fluorescence with a spatially defined light beam of a given wavelength. https://www.leica-microsystems.com//science-lab/photoactivatable-photoconvertible-and-photoswitchable-fluorescent-proteins/ Thu, 04 May 2017 11:31:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/14918 Super-Resolution Live-Cell Imaging Image Restoration and Deconvolution A Bright Dye for Live-Cell STED Microscopy The aim of cell biology is to study smallest details on a cellular level preferably in a live cell experiment. By providing fast and direct super-resolution, STED (Stimulated Emission Depletion) microscopy is the perfect tool for studying cellular details in the nanometer range in vivo. https://www.leica-microsystems.com//science-lab/a-bright-dye-for-live-cell-sted-microscopy/ Mon, 19 Jan 2015 15:47:00 +0000 PhD Stefan Pitsch, PhD Isabelle Köster https://www.leica-microsystems.com/4758 Live-Cell Imaging Neuroscience Ratiometric Imaging Many fundamental functions of a cell strongly depend on delicate, but nevertheless dynamic balances of ions (e.g. calcium, magnesium), voltage potentials and pH between the cell’s cytosol and the surrounding extracellular space. Ratiometric imaging allows reliable estimations of ion concentrations and pH or voltage changes by measuring fluorophore emission shifts. https://www.leica-microsystems.com//science-lab/ratiometric-imaging/ Sun, 11 Dec 2011 23:00:00 +0000 Dr. Thomas Veitinger