Leica Science Lab - Tag : Endocytosis https://www.leica-microsystems.com//science-lab/tag/tags/endocytosis/show/Tag/ Article tagged with Endocytosis en-US https://www.leica-microsystems.com/18905 Super-Resolution Neuroscience Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been challenged, suggesting that their recycling ability may differ greatly. Botulinum neurotoxin type-A (BoNT/A) is a highly potent neurotoxin that is internalized in synaptic vesicles at motor nerve terminals and induces flaccid paralysis. Recently, BoNT/A was also shown to undergo retrograde transport, suggesting it might enter a specific pool of synaptic vesicles with a retrograde trafficking fate. Using high-resolution microscopy techniques including electron microscopy and single molecule imaging, we found that the BoNT/A binding domain is internalized within a subset of vesicles that only partially co-localize with cholera toxin B-subunit and have markedly reduced VAMP2 immunoreactivity. https://www.leica-microsystems.com//science-lab/botulinum-neurotoxin-type-a-enters-a-non-recycling-pool-of-synaptic-vesicles/ Wed, 19 Oct 2016 11:51:00 +0000 https://www.leica-microsystems.com/14658 Super-Resolution Neuroscience A New Probe for Super-Resolution Imaging of Membranes Elucidates Trafficking Pathways The molecular composition of the organelles involved in membrane recycling is difficult to establish as a result of the absence of suitable labeling tools. We introduce in this paper a novel probe, named membrane-binding fluorophore-cysteine-lysine-palmitoyl group (mCLING), which labels the plasma membrane and is taken up during endocytosis. https://www.leica-microsystems.com//science-lab/a-new-probe-for-super-resolution-imaging-of-membranes-elucidates-trafficking-pathways/ Fri, 17 Oct 2014 12:16:00 +0000 https://www.leica-microsystems.com/13373 Super-Resolution Neuroscience Synaptic Vesicle Recycling: Steps and Principles Synaptic vesicle recycling is one of the bestā€studied cellular pathways. Many of the proteins involved are known, and their interactions are becoming increasingly clear. However, as for many other pathways, it is still difficult to understand synaptic vesicle recycling as a whole. https://www.leica-microsystems.com//science-lab/synaptic-vesicle-recycling-steps-and-principles/ Mon, 26 May 2014 09:30:00 +0000 Prof. Silvio Rizzoli https://www.leica-microsystems.com/5175 TIRF Microscopy Live-Cell Imaging Protein Transport Processes at the Apical Membrane of Polarized Epithelial Cells Due to their special role in organ function and the exchange of biological components some body cells developed certain polarization characteristics. These are reflected in differences of their plasma membrane composition. The essential and fascinating task of polarized protein transport in epithelial cells is to get the right protein into the right membrane. https://www.leica-microsystems.com//science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells/ Sun, 11 Mar 2012 23:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/14848 Confocal Microscopy Apical Cargo Traverses Endosomal Compartments on the Passage to the Cell Surface Epithelial polarity is based on intracellular sorting machinery that maintains the asymmetric distribution of lipids and proteins to the cell surface. Dependent on their lipid raft affinity, newly synthesized apical polypeptides are segregated into distinct vesicle populations subsequent to the passage through the Golgi apparatus. https://www.leica-microsystems.com//science-lab/apical-cargo-traverses-endosomal-compartments-on-the-passage-to-the-cell-surface/ Mon, 15 Sep 2008 17:25:00 +0000 https://www.leica-microsystems.com/3006 Live-Cell Imaging TIRF Microscopy Exploring Cell Logistics Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a potential apical sorting receptor. https://www.leica-microsystems.com//science-lab/exploring-cell-logistics/ Fri, 02 Nov 2007 11:29:00 +0000 Prof. Ralf Jacob, Dipl. Biol. Dominik Schneider