Leica Science Lab - Tag : Evanescent Field https://www.leica-microsystems.com//science-lab/tag/tags/evanescent-field/show/Tag/ Article tagged with Evanescent Field en-US https://www.leica-microsystems.com/6282 TIRF Microscopy TIRF Publication List This monthly updated references list presents current papers using Leica AM TIRF in the major application fields for TIRF microscopy. https://www.leica-microsystems.com//science-lab/tirf-publication-list/ Mon, 19 Dec 2016 09:29:00 +0000 https://www.leica-microsystems.com/13946 TIRF Microscopy Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique is used for, explains the principles of the evanescent wave, gives many examples of different microscope configurations used in TIRF, and shows how polarized light TIRF can be used to image membrane orientation. https://www.leica-microsystems.com//science-lab/video-talk-by-daniel-axelrod-total-internal-reflection-fluorescence-tirf-microscopy/ Mon, 09 Mar 2015 08:45:00 +0000 https://www.leica-microsystems.com/5945 TIRF Microscopy Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the evanescent wave, which arises from the glass/water or glass/specimen interface, is that its propagation in z-direction gradually degrades, limiting its penetration depth into the specimen to some hundred nanometers. https://www.leica-microsystems.com//science-lab/controlling-the-tirf-penetration-depth-is-mandatory-for-reproducible-results/ Tue, 24 Apr 2012 22:00:00 +0000 Dr. Thomas Veitinger https://www.leica-microsystems.com/5403 TIRF Microscopy Live-Cell Imaging Widefield Microscopy Total Internal Reflection Fluorescence (TIRF) Microscopy Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy delivers images with an outstandingly high axial resolution below 100 nm. This allows the observation of membrane-associated processes. https://www.leica-microsystems.com//science-lab/total-internal-reflection-fluorescence-tirf-microscopy/ Sun, 11 Mar 2012 23:00:00 +0000 Wymke Ockenga https://www.leica-microsystems.com/5405 Live-Cell Imaging TIRF Microscopy Widefield Microscopy Applications of TIRF Microscopy in Life Science Research The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or lasers, the evanescent field only penetrates the specimen by about 100 nm starting from the coverslip/medium interface. https://www.leica-microsystems.com//science-lab/applications-of-tirf-microscopy-in-life-science-research/ Sun, 11 Mar 2012 23:00:00 +0000 Wymke Ockenga, Dr. Thomas Veitinger