Leica Science Lab - Tag : Fluorochromes https://www.leica-microsystems.com//science-lab/tag/tags/fluorochromes/show/Tag/ Article tagged with Fluorochromes en-US https://www.leica-microsystems.com/13942 Fluorescence Microscopy Quantitative Fluorescence Video Talk by Roger Tsien: Fluorescent Protein Indicators In this talk, Roger Tsien discusses how fluorescent proteins have been turned into indicators for a wide variety of biological molecules, including pH, ions, redox potential, and signaling molecules like phosphoinositides. The talk also covers reporters used to measure the activity of enzymes like kinases, phosphatases, and proteases. It covers both single proteins whose intensity or wavelength change, as well as reporters using resonance energy transfer (FRET). https://www.leica-microsystems.com//science-lab/video-talk-by-roger-tsien-fluorescent-protein-indicators/ Tue, 16 Aug 2016 10:29:00 +0000 PhD Roger Y. Tsien https://www.leica-microsystems.com/16035 Fluorescence Microscopy Live-Cell Imaging Basics in Microscopy Fluorescent Proteins Illuminate Cell Biology Green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria and GFP-like fluorescent proteins from other animals have had an important role in the technical innovations that have driven these advances. This poster provides a comprehensive user's guide to fluorescent proteins and sensors , their key properties and the cell biological questions to which they can be applied. https://www.leica-microsystems.com//science-lab/fluorescent-proteins-illuminate-cell-biology/ Fri, 18 Sep 2015 09:03:00 +0000 https://www.leica-microsystems.com/15458 Confocal Microscopy Fluorescence Microscopy Improving Axial Resolution in Confocal Microscopy with New High Refractive Index Mounting Media Resolution, high signal intensity and elevated signal to noise ratio (SNR) are key issues for biologists who aim at studying the localisation of biological structures at the cellular and subcellular levels using confocal microscopy. The resolution required to separate sub-cellular biological structures is often near to the resolving power of the microscope. https://www.leica-microsystems.com//science-lab/improving-axial-resolution-in-confocal-microscopy-with-new-high-refractive-index-mounting-media/ Thu, 11 Jun 2015 13:04:00 +0000 https://www.leica-microsystems.com/15295 Fluorescence Microscopy How to Prepare Your Specimen for Immunofluorescence Microscopy Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM, Epifluorescence, TIRF, GSDIM), depending on the application or the researcher’s interest. Meanwhile, IF has become indispensable for a large number of research groups which have at least access to a simple fluorescence microscope. https://www.leica-microsystems.com//science-lab/how-to-prepare-your-specimen-for-immunofluorescence-microscopy/ Mon, 13 Apr 2015 14:22:00 +0000 Florian Hoff https://www.leica-microsystems.com/14080 Fluorescence Microscopy Live-Cell Imaging Spectral and Structural Comparison Between Bright and Dim Green Fluorescent Proteins in Amphioxus The cephalochordate Amphioxus naturally co-expresses fluorescent proteins (FPs) with different brightness, which thus offers the rare opportunity to identify FP molecular feature/s that are associated with greater/lower intensity of fluorescence. Here, we describe the spectral and structural characteristics of green FP (bfloGFPa1) with perfect (100%) quantum efficiency yielding to unprecedentedly-high brightness, and compare them to those of co-expressed bfloGFPc1 showing extremely-dim brightness due to low (0.1%) quantum efficiency. https://www.leica-microsystems.com//science-lab/spectral-and-structural-comparison-between-bright-and-dim-green-fluorescent-proteins-in-amphioxus/ Thu, 28 Aug 2014 15:54:00 +0000 https://www.leica-microsystems.com/9350 Fluorescence Microscopy Confocal Microscopy Live-Cell Imaging Handbook of Optical Filters for Fluorescence Microscopy Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and an optic that might be appropriate and optimal for one is both inappropriate and sub-optimal for another. https://www.leica-microsystems.com//science-lab/handbook-of-optical-filters-for-fluorescence-microscopy/ Tue, 06 Aug 2013 07:59:00 +0000 BSc. Jay Reichman https://www.leica-microsystems.com/5890 Fluorescence Microscopy Basics in Microscopy Basic Principles of Luminescence There are a lot of light-emitting processes occurring in nature. Luminescence is an umbrella term for those kinds of events where light emission is not the result of high temperatures. This article depicts the different forms of luminescences and goes into detail in the case of fluorescence. Relevant technical terms describing a fluorochrome, like quenching, bleaching or quantum yield, are explained in the second part of the article to give detailed insights into basic characteristics of fluorescent molecules. https://www.leica-microsystems.com//science-lab/basic-principles-of-luminescence/ Mon, 09 Apr 2012 22:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/4182 Fluorescence Microscopy Basics in Microscopy An Introduction to Fluorescence Fluorescence is widely used in microscopy and an important tool for observing the distribution of specific molecules. Most molecules in cells do not fluoresce. They therefore have to be marked with fluorescing molecules called fluorochromes. https://www.leica-microsystems.com//science-lab/an-introduction-to-fluorescence/ Tue, 31 May 2011 13:29:00 +0000 Wymke Ockenga