Leica Science Lab - Tag : Immersion Freezing https://www.leica-microsystems.com//science-lab/tag/tags/immersion-freezing/show/Tag/ Article tagged with Immersion Freezing en-US https://www.leica-microsystems.com/15081 EM Sample Preparation CLEM New Insights into Cilia and Flagella by Cryo-EM Cilia and flagella were the first organelles to be discovered and have been studied for centuries. However, their essential role in humans and how ciliary defects cause diseases are still not well understood. Cryo-EM has recently shed new light on their inner workings and solved some long-standing mysteries, only to raise new questions on how cilia and flagella function. https://www.leica-microsystems.com//science-lab/new-insights-into-cilia-and-flagella-by-cryo-em/ Mon, 16 Feb 2015 09:25:00 +0000 PhD Thomas Heuser https://www.leica-microsystems.com/15104 EM Sample Preparation Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications A well established usage case for cryo-TEM is three-dimensional reconstruction of isolated macromolecules, virus particles, or filaments. On one hand, these approaches are based on averaging of repetitive structures – either due to numerous identical molecules, repetitive patterns on a filament, or symmetries, to reduce the noise inherent to cryo-TEM. https://www.leica-microsystems.com//science-lab/immersion-freezing-for-cryo-transmission-electron-microscopy-applications/ Mon, 09 Feb 2015 14:51:00 +0000 Dr. Guenter Resch https://www.leica-microsystems.com/15102 EM Sample Preparation Immersion Freezing for Cryo-Transmission Electron Microscopy: Fundamentals The high vacuum required in a transmission electron microscope (TEM) greatly impairs the ability to study specimens naturally occurring in an aqueous phase: exposing "wet" specimens to a pressure significantly lower than the vapour pressure of water will lead to the water phase boiling off rapidly in the column, with devastating consequences for the structure of the specimen. Hence, various methods to dry specimens before inspection are employed in conventional TEM – a preparative step often associated with artifacts limiting the significance of the results. https://www.leica-microsystems.com//science-lab/immersion-freezing-for-cryo-transmission-electron-microscopy-fundamentals/ Mon, 09 Feb 2015 14:41:00 +0000 Dr. Guenter Resch https://www.leica-microsystems.com/9555 EM Sample Preparation Immersion Freezing of Suspended Particles and Cells for Cryo-Electron Microscopy Immersion freezing of thin aqueous specimens is an essential preparation technique for cryo-transmission electron microscopy (cryo-TEM), aiming to preserve fragile biological structures such as molecules and cells in their hydrated environment for a close-to-native visualization. For successful experiments, vitreous ice must be produced, surface contamination must be avoided, and, most important, the natural state of the structure must be preserved. https://www.leica-microsystems.com//science-lab/immersion-freezing-of-suspended-particles-and-cells-for-cryo-electron-microscopy/ Fri, 12 Apr 2013 11:48:00 +0000 Dr. Guenter Resch, Marlene Brandstetter, Lisa Königsmaier, Edith Urban, Angela M. Pickl-Herk