Leica Science Lab - Tag : Inflammation https://www.leica-microsystems.com//science-lab/tag/tags/inflammation/show/Tag/ Article tagged with Inflammation en-US https://www.leica-microsystems.com/18172 Multiphoton Microscopy Fluorescence Microscopy Chronic Stress in Mice Remodels Lymph Vasculature to Promote Tumour Cell Dissemination Chronic stress induces signalling from the sympathetic nervous system (SNS) and drives cancer progression, although the pathways of tumour cell dissemination are unclear. Here we show that chronic stress restructures lymphatic networks within and around tumours to provide pathways for tumour cell escape. We show that VEGFC derived from tumour cells is required for stress to induce lymphatic remodelling and that this depends on COX2 inflammatory signalling from macrophages. Pharmacological inhibition of SNS signalling blocks the effect of chronic stress on lymphatic remodelling in vivo and reduces lymphatic metastasis in preclinical cancer models and in patients with breast cancer. https://www.leica-microsystems.com//science-lab/chronic-stress-in-mice-remodels-lymph-vasculature-to-promote-tumour-cell-dissemination/ Tue, 07 Feb 2017 09:52:00 +0000 https://www.leica-microsystems.com/18994 Fluorescence Microscopy Live-Cell Imaging Widefield Microscopy Image Restoration and Deconvolution Chronic Inflammation Under the Microscope In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can be examined from a cellular level to whole organisms. This article presents several widefield microscopy applications such as immunofluorescence, live-cell imaging, histology, and ratiometric analysis to get insight into the development of chronic inflammation, the related diseases, and their treatment. https://www.leica-microsystems.com//science-lab/chronic-inflammation-under-the-microscope/ Mon, 09 Jan 2017 17:42:00 +0000 M.Sc. Jan Neumann, M.Sc. Anne Scherhag, Susanne Otten, Ph.D. Fangxia Shen, Anna Lena Leifke, Dr. Udo Birk, Dr. Christoph Greb, Dr. Kurt Lucas, Prof. Dr. Dr. Christoph Cremer https://www.leica-microsystems.com/18730 Confocal Microscopy Live-Cell Imaging Adeno-associated Viral Vectors do not Efficiently Target Muscle Satellite Cells Adeno-associated viral (AAV) vectors are becoming an important tool for gene therapy of numerous genetic and other disorders. Several recombinant AAV vectors (rAAV) have the ability to transduce striated muscles in a variety of animals following intramuscular and intravascular administration, and have attracted widespread interest for therapy of muscle disorders such as the muscular dystrophies. Here we examined the relative ability of rAAV vectors derived from AAV6 to target myoblasts, myocytes, and myotubes in culture and satellite cells and myofibers in vivo. AAV vectors are able to transduce proliferating myoblasts in culture, albeit with reduced efficiency relative to postmitotic myocytes and myotubes. In contrast, quiescent satellite cells are refractory to transduction in adult mice. https://www.leica-microsystems.com//science-lab/adeno-associated-viral-vectors-do-not-efficiently-target-muscle-satellite-cells/ Mon, 05 Sep 2016 05:58:00 +0000 https://www.leica-microsystems.com/18102 Super-Resolution The Actin Cytoskeleton Modulates the Activation of iNKT Cells by Segregating CD1d Nanoclusters on Antigen-Presenting Cells The ability of invariant natural killer T (iNKT) cells to recognize endogenous antigens represents a distinct immune recognition strategy, which underscores the constitutive memory phenotype of iNKT cells and their activation during inflammatory conditions. By using superresolution microscopy, we show that CD1d molecules form nanoclusters at the cell surface of APCs, and their size and density are constrained by the actin cytoskeleton. https://www.leica-microsystems.com//science-lab/the-actin-cytoskeleton-modulates-the-activation-of-inkt-cells-by-segregating-cd1d-nanoclusters-on-antigen-presenting-cells/ Wed, 10 Aug 2016 13:28:00 +0000 https://www.leica-microsystems.com/18115 White Confocal Contributions of Microbiome and Mechanical Deformation to Intestinal Bacterial Overgrowth and Inflammation in a Human Gut-on-a-Chip A human gut-on-a-chip microdevice was used to coculture multiple commensal microbes in contact with living human intestinal epithelial cells for more than a week in vitro and to analyze how gut microbiome, inflammatory cells, and peristalsis-associated mechanical deformations independently contribute to intestinal bacterial overgrowth and inflammation. This in vitro model replicated results from past animal and human studies, including demonstration that probiotic and antibiotic therapies can suppress villus injury induced by pathogenic bacteria. https://www.leica-microsystems.com//science-lab/contributions-of-microbiome-and-mechanical-deformation-to-intestinal-bacterial-overgrowth-and-inflammation-in-a-human-gut-on-a-chip/ Fri, 05 Aug 2016 16:29:00 +0000 https://www.leica-microsystems.com/18176 White Confocal ERK2 and Akt are Negative Regulators of Insulin and Tumor Necrosis Factor-α Stimulated VCAM-1 Expression in Rat Aorta Endothelial Cells Diabetes is quickly becoming the most widespread disorder in the Western world. Among the most prevalent effects of diabetes is atherosclerosis, which in turn is driven in part by inflammation. Both insulin and Tumor Necrosis Factor-alpha (TNFα) increase the presence of Vascular Cellular Adhesion Molecule-1 (VCAM-1) expression. The aim of this study is to determine the effects of downregulating Extracellular signal-Regulated Kinase-2 (ERK2) and Akt on insulin and TNFa-stimulated VCAM-1 expression. https://www.leica-microsystems.com//science-lab/erk2-and-akt-are-negative-regulators-of-insulin-and-tumor-necrosis-factor-a-stimulated-vcam-1-expression-in-rat-aorta-endothelial-cells/ Wed, 01 Jun 2016 12:14:00 +0000