Leica Science Lab - Tag : Infrared https://www.leica-microsystems.com//science-lab/tag/tags/infrared/show/Tag/ Article tagged with Infrared en-US https://www.leica-microsystems.com/4421 Coherent Raman Scattering (CRS) Coherent Raman Scattering Microscopy Publication List CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their molecules. The two most important CRS techniques are Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). The biochemical image contrast of CRS is in many ways complementary to the molecular contrast obtained in fluorescence microscopy. A second crucial advantage of these methods is that they preserve the specimen/sample in a near pristine state. This reference list presents current and basic papers on CRS microscopy. https://www.leica-microsystems.com//science-lab/cars-publication-list/ Sun, 26 May 2019 22:00:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/19766 Multiphoton Microscopy Multiphoton Microscopy – a Satisfied Wish List The colorful picture shows colon tumor cells, fluorescently labelled and lineage traced from a multicolor tracer. The gray color codes for the second harmonic generation (SHG) signal from Collagen 1. Lineage traced tumor cells are shown in magenta, blue, green, yellow and red. All channels were recorded with two-photon excitation, using the SP8 DIVE by Leica Microsystems. Sample and image were kindly provided by J. van Rheenen, H. Snippert, Utrecht (the Nederlands,) and I. Steinmetz, Leica Microsystems Mannheim. https://www.leica-microsystems.com//science-lab/multiphoton-microscopy-a-satisfied-wish-list/ Wed, 25 Oct 2017 08:49:00 +0000 Dr. Rolf T. Borlinghaus, Ph.D. Bettina Griesshaber https://www.leica-microsystems.com/19620 Multiphoton Microscopy Laser Beam Shaping for Multicolor Multiphoton Microscopy Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely tunable non-descanning detector and an ingenious beam manipulating device VBE. The variable beam expander offers free tuning of both beam diameter and axial IR-correction for up to four IR beams simultaneously. https://www.leica-microsystems.com//science-lab/laser-beam-shaping-for-multicolor-multiphoton-microscopy/ Thu, 06 Jul 2017 08:24:00 +0000 Dr. Rolf T. Borlinghaus, Manuel Kremer https://www.leica-microsystems.com/14920 Multiphoton Microscopy CLEM Live-Cell Imaging Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points Cancer research unsing multiphoton microscopy and 3D electron microscopy. Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient events at high resolution. Performing correlative microscopy in complex and bulky samples such as an entire living organism is a time-consuming and error-prone task. https://www.leica-microsystems.com//science-lab/correlating-intravital-multi-photon-microscopy-to-3d-electron-microscopy-of-invading-tumor-cells-using-anatomical-reference-points/ Fri, 24 Apr 2015 13:06:00 +0000 https://www.leica-microsystems.com/9374 Coherent Raman Scattering (CRS) Unmask the Hidden without Probes: CARS Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul Ehrlich, had the idea that something that stains specifically should also kill specifically – which was associated with the term “magic bullet”, the essential idea of chemotherapy. His group discovered Salvarsan, a tailored drug against syphilis – though not specific enough not to cause substantial side effects. Screening many fluorescent dyes led to a long list of stainings which are used in histology, including dyes like DAPI or hematoxylin and eosin. https://www.leica-microsystems.com//science-lab/unmask-the-hidden-without-probes-cars/ Thu, 21 Mar 2013 23:00:00 +0000 Dr. Rolf T. Borlinghaus, Dr. Stefanie Degenhartt https://www.leica-microsystems.com/6654 Multiphoton Microscopy Principles of Multiphoton Microscopy for Deep Tissue Imaging Basics of multiphoton microscopy. This interactive tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking advantage of the reduced scattering of longer wavelengths. This makes multiphoton imaging the perfect tool for deep tissue imaging in thick sections and living animals. https://www.leica-microsystems.com//science-lab/principles-of-multiphoton-microscopy-for-deep-tissue-imaging/ Thu, 23 Aug 2012 22:00:00 +0000 Dr. Andrea Mülter https://www.leica-microsystems.com/4662 Coherent Raman Scattering (CRS) An Introduction to CARS Microscopy CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which are based on vibrational contrast and chemical selectivity. The crucial advantage of this method is that the sample remains almost unaffected. With CARS, new samples that could not be stained due to unavailability of the appropriate dye are now accessible. https://www.leica-microsystems.com//science-lab/an-introduction-to-cars-microscopy/ Fri, 04 Nov 2011 14:36:46 +0000 Dr. Stefanie Degenhartt https://www.leica-microsystems.com/4592 Coherent Raman Scattering (CRS) Step by Step Guide to the Molecular Basics of CARS Microscopy CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial advantage of this method is that the sample remains almost unaffected. This tutorial explains the molecular basics on Coherent Anti-Stokes Raman Scattering. https://www.leica-microsystems.com//science-lab/step-by-step-guide-to-the-molecular-basics-of-cars-microscopy/ Tue, 01 Nov 2011 17:17:00 +0000 Dr. Stefanie Degenhartt https://www.leica-microsystems.com/2743 Coherent Raman Scattering (CRS) Multiphoton Microscopy Confocal Microscopy CARS and Confocal The most important drawback of single-photon and multiphoton confocal microscopy is the need to label the specimen. CARS (Coherent Anti-Stokes Raman Spectroscopy) addresses this issue because it is non-toxic, non-destructive, and minimally invasive. https://www.leica-microsystems.com//science-lab/cars-and-confocal/ Mon, 01 Nov 2010 23:00:00 +0000 Dr. Stefanie Degenhartt, Ph.D. Vanessa Lurquin