Leica Science Lab - Tag : Kidney Disease https://www.leica-microsystems.com//science-lab/tag/tags/kidney-disease/show/Tag/ Article tagged with Kidney Disease en-US https://www.leica-microsystems.com/18860 Webinar: Laser Microdissection in Cancer Research – Mutation Analysis Workflow with Pure Cancer Material Cancer can affect various organs and is caused by mutations of the DNA. A prerequisite, to explore and understand underlying gene-mutations involved in the development of a definite type of cancer, is the extraction of pure sample material, which is challenging. In this webinar, we will present how to extract 100% pure cancer tissue for DNA analysis with laser microdissection (LMD). Using tissue samples from human kidney cancer patients as an example, this webinar will provide an overview of the practical considerations when preparing a workflow to obtain highly pure material with the LMD microscope for further molecular analysis. https://www.leica-microsystems.com//science-lab/webinar-laser-microdissection-in-cancer-research-mutation-analysis-workflow-with-pure-cancer-material/ Tue, 04 Oct 2016 08:57:00 +0000 PhD Tamara Straube, Prof. Ralf Jacob https://www.leica-microsystems.com/18779 Multiphoton Microscopy BABB Clearing and Imaging for High Resolution Confocal Microscopy: Counting and Sizing Kidney Cells in the 21st Century Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective. Understanding kidney microanatomy is key to detecting and identifying early events in kidney disease. Improvements in tissue clearing and imaging have been crucial in this field, and now we report on a novel, time-efficient method to study podocyte depletion in renal glomeruli using a combination of immunofluorescence, optical clearing, confocal microscopy and 3D analysis. https://www.leica-microsystems.com//science-lab/babb-clearing-and-imaging-for-high-resolution-confocal-microscopy-counting-and-sizing-kidney-cells-in-the-21st-century/ Tue, 13 Sep 2016 09:03:00 +0000 Ph.D. Victor G. Puelles, Prof. John F. Bertram, MSc Stephen Firth, Prof. Ian Harper https://www.leica-microsystems.com/13257 Multiphoton Microscopy Live-Cell Imaging Tracking Glomerular Fate Over Long Time Distances Multi-channel multiphoton microscopy with dedicated optics for CLARITY. The glomerular filtration barrier (GFB) is a complex spatial structure within the kidney glomerulis where ultrafiltration takes place. Podocytes are critical elements of the GFB and take part in the filtration process. They have been shown to be involved in the development of kidney diseases. However, due to technical limitations, the mechanism of glomerular pathology is not well understood. https://www.leica-microsystems.com//science-lab/tracking-glomerular-fate-over-long-time-distances/ Mon, 14 Apr 2014 12:32:00 +0000 M.D., Ph.D. Janos Peti-Peterdi, PhD Isabelle Köster