Leica Science Lab - Tag : Membrane Protein https://www.leica-microsystems.com//science-lab/tag/tags/membrane-protein/show/Tag/ Article tagged with Membrane Protein en-US https://www.leica-microsystems.com/25094 Quantitative Fluorescence Single-residue bioorthogonal labeling of G protein-coupled receptors (GPCRs) in live cells for quantitative analysis To perform quantitative studies of membrane bound G protein-coupled receptors (GPCRs) with high-performance microscopy, the proteins must first be labeled with bright and photostable dyes. The labeling method should be minimally invasive to avoid perturbing the GPCR function and result in quantitative yields to allow stoichiometric data analysis. For the precise quantification of dye yields, a method based on fluorescence fluctuation microscopy (molecular brightness) has been developed. The method can extract the number of labeling sites at the single-cell level. Because the reported technique is performed using a commercially available microscope, the approach can be used for the general study of membrane proteins with fluorescence microscopy. https://www.leica-microsystems.com//science-lab/single-residue-bioorthogonal-labeling-of-g-protein-coupled-receptors-gpcrs-in-live-cells-for-quantitative-analysis/ Wed, 26 Jun 2019 10:03:00 +0000 PhD James DeRose, PhD Paolo Annibale https://www.leica-microsystems.com/18958 Super-Resolution Image Restoration and Deconvolution The Bif-1-Dynamin 2 Membrane Fission Machinery Regulates Atg9-Containing Vesicle Generation at the Rab11-Positive Reservoirs Application example of HyVolution Super-Resolution - Atg9 is a multispanning transmembrane protein that is required for autophagosome formation. During autophagy, vesicles containing Atg9 are generated through an unknown mechanism and delivered to the autophagosome formation sites. We have previously reported that Atg9-containing membranes undergo continuous tubulation and fission during nutrient starvation in a manner dependent on the curvature-inducing protein Bif-1/Sh3glb1. Here, we identify Dynamin 2 (DNM2) as a Bif-1-interacting protein that mediates the fission of Atg9-containing membranes during autophagy. https://www.leica-microsystems.com//science-lab/the-bif-1-dynamin-2-membrane-fission-machinery-regulates-atg9-containing-vesicle-generation-at-the-rab11-positive-reservoirs/ Wed, 25 Jan 2017 13:00:00 +0000 https://www.leica-microsystems.com/16174 Super-Resolution Quantitative Fluorescence Live-Cell Imaging Cortical Actin Networks Induce Spatio-temporal Confinement of Phospholipids in the Plasma Membrane – A Minimally Invasive Investigation by STED-FCS Important discoveries in the last decades have changed our view of the plasma membrane organisation. Specifically, the cortical cytoskeleton has emerged as a key modulator of the lateral diffusion of membrane proteins. Cytoskeleton-dependent compartmentalised lipid diffusion has been proposed, but this concept remains controversial because this phenomenon has thus far only been observed with artefact-prone probes in combination with a single technique: single particle tracking. https://www.leica-microsystems.com//science-lab/cortical-actin-networks-induce-spatio-temporal-confinement-of-phospholipids-in-the-plasma-membrane-a-minimally-invasive-investigation-by-sted-fcs/ Fri, 24 Jul 2015 13:02:00 +0000 Débora Machado Andrade https://www.leica-microsystems.com/15241 Super-Resolution Live-Cell Imaging Multi-protein Assemblies Underlie the Mesoscale Organization of the Plasma Membrane Most proteins have uneven distributions in the plasma membrane. Broadly speaking, this may be caused by mechanisms specific to each protein, or may be a consequence of a general pattern that affects the distribution of all membrane proteins. https://www.leica-microsystems.com//science-lab/multi-protein-assemblies-underlie-the-mesoscale-organization-of-the-plasma-membrane/ Thu, 09 Jul 2015 13:44:00 +0000 https://www.leica-microsystems.com/14796 Super-Resolution Multilayered Polyelectrolyte Microcapsules: Interaction with the Enzyme Cytochrome C Oxidase Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. https://www.leica-microsystems.com//science-lab/multilayered-polyelectrolyte-microcapsules-interaction-with-the-enzyme-cytochrome-c-oxidase/ Tue, 05 May 2015 17:31:00 +0000