Leica Science Lab - Tag : Microbiology https://www.leica-microsystems.com//science-lab/tag/tags/microbiology/show/Tag/ Article tagged with Microbiology en-US https://www.leica-microsystems.com/19635 Fluorescence Microscopy Live-Cell Imaging Stereo Microscopy Widefield Microscopy Real Time Observation of Neutrophil White Blood Cell Recruitment to Bacterial Infection In Vivo The zebrafish (Danio rerio) is an emerging vertebrate model organism to study infection. The transparent larva comprises a fully functional innate immune system and enables live imaging of fluorescent immune cells in transgenic animals. Zebrafish infection models have been developed for both the human bacterial pathogen Shigella flexneri and the natural fish bacterial pathogen Mycobacterium marinum. Importantly, whilst S. flexneri causes acute infection and is typically used as an inflammatory paradigm, M. marinum causes a chronic disease similar to tuberculosis in humans. Here, we use real time fluorescence microscopy to image transgenic zebrafish larvae with neutrophils (granulocyte white blood cells) expressing the green fluorescent protein eGFP. https://www.leica-microsystems.com//science-lab/real-time-observation-of-neutrophil-white-blood-cell-recruitment-to-bacterial-infection-in-vivo/ Thu, 27 Jul 2017 11:20:00 +0000 MSc Alexandra R. Willis, MSc Vincenzo Torraca, PhD Serge Mostowy, PhD James DeRose https://www.leica-microsystems.com/18115 White Confocal Contributions of Microbiome and Mechanical Deformation to Intestinal Bacterial Overgrowth and Inflammation in a Human Gut-on-a-Chip A human gut-on-a-chip microdevice was used to coculture multiple commensal microbes in contact with living human intestinal epithelial cells for more than a week in vitro and to analyze how gut microbiome, inflammatory cells, and peristalsis-associated mechanical deformations independently contribute to intestinal bacterial overgrowth and inflammation. This in vitro model replicated results from past animal and human studies, including demonstration that probiotic and antibiotic therapies can suppress villus injury induced by pathogenic bacteria. https://www.leica-microsystems.com//science-lab/contributions-of-microbiome-and-mechanical-deformation-to-intestinal-bacterial-overgrowth-and-inflammation-in-a-human-gut-on-a-chip/ Fri, 05 Aug 2016 16:29:00 +0000 https://www.leica-microsystems.com/5321 Education Basics in Microscopy Antimicrobial Coating for Educational Microscopes Bacteria are part of our world. There are countless numbers of them in the human body and they are completely harmless. But in people with a weak immune system or at the wrong place they can cause serious illness. Educational microscopes that pass through many hands are potential breeding grounds for germs. To solve this problem... https://www.leica-microsystems.com//science-lab/antimicrobial-coating-for-educational-microscopes/ Wed, 29 Feb 2012 23:00:00 +0000 M. A. Janika Wiesner, Christoph Fankhauser