Leica Science Lab - Tag : Molecular Biology https://www.leica-microsystems.com//science-lab/tag/tags/molecular-biology/show/Tag/ Article tagged with Molecular Biology en-US https://www.leica-microsystems.com/26832 Quantitative Imaging Imaging Intracellular Temperature using Fluorescence Lifetime Imaging Microscopy (FLIM) This video shows the presentation of Dr. Kohki Okabe given at the 9th Congress of the Federation of Asian and Oceanian Physiological Society (FAOPS) held in Kobe, Japan on 30 March 2019. Dr. Okabe is an Assistant Professor and JST-PRESTO Researcher with the Laboratory of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo. https://www.leica-microsystems.com//science-lab/imaging-intracellular-temperature-using-fluorescence-lifetime-imaging-microscopy-flim/ Thu, 07 Nov 2019 09:10:00 +0000 PhD Kohki Okabe, Dr. Giulia Ossato https://www.leica-microsystems.com/26294 Quantitative Imaging Development of fluorescence lifetime imaging microscopy (FLIM) and its relevance for functional imaging Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager functional imaging, during Leica Microsystems Meets Science 2019 in Mannheim, Germany. They had an inspiring chat about fluorescence lifetime imaging microscopy (FLIM). The technological development of FLIM and its relevance for functional imaging in terms of studying redox states was discussed. Prof. Periasamy also talked about his research on metabolism and dysfunction in live cells. He stated that one of the central developments in the last few decades is the application of lifetime contrast to gather information about molecular interactions and function. https://www.leica-microsystems.com//science-lab/development-of-fluorescence-lifetime-imaging-microscopy-flim-and-its-relevance-for-functional-imaging/ Wed, 28 Aug 2019 10:13:00 +0000 Professor Ammasi Periasamy, Dr. Giulia Ossato https://www.leica-microsystems.com/19872 Super-Resolution Abstracts of the 7th European Super-Resolution User-Club Meeting The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in Prague. Keeping the event close to science is one of the founding principles of the event, allowing all participants to network, share and explore exciting new super-resolution and nanoscopy applications. Central to this are the scientific talks given during the meeting, with this cutting-edge microscopy technique as their central theme. A wide selection of topics were covered, prompting interesting discussions during the workshops. https://www.leica-microsystems.com//science-lab/abstracts-of-the-7th-european-super-resolution-user-club-meeting/ Sun, 17 Dec 2017 23:00:00 +0000 Prof. DrSc. Pavel Hozák, Prof. Dr. Torsten Ochsenreiter, Dr. Martin Offterdinger, Dr. Jordi Andilla, Ph.D. Marc van Zandvoort, Prof. Christian Eggeling, Dr. Susan Cox, Dr. Eugene Katrukha, Dr. Jindřiška Fišerová, Dr. Camille Boutin https://www.leica-microsystems.com/19239 CRISPR-Cas9 Video: Genome Engineering with CRISPR-Cas9 Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studies that were previously unachievable. https://www.leica-microsystems.com//science-lab/video-genome-engineering-with-crispr-cas9/ Fri, 09 Jun 2017 06:50:00 +0000 https://www.leica-microsystems.com/19530 CRISPR-Cas9 Video: Genome Editing with CRISPR-Cas9 Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studies that were previously unachievable. https://www.leica-microsystems.com//science-lab/video-genome-editing-with-crispr-cas9/ Thu, 08 Jun 2017 06:50:00 +0000 https://www.leica-microsystems.com/19390 Laser Microdissection Correlation of Phenotype and Genotype – microscopic samples and their biomolecular backgrounds Seeing is believing, this is what makes microscopes, imaging systems, or box-type imagers so important for researchers in many different fields like pathology research, cancer research, neuroscience, and developmental research. Correlation of phenotype (what you can see) and genotype (genetic background) is highly desired but often biased by whole tissue approaches as no real solution is available to reliably separate the single cells of interest and surrounding tissue. Thus, results of DNA mutation analysis (sequencing), gene expression profiling (quantitative real-time PCR, qPCR, microarray, digital PCR), next generation sequencing (NGS) or mass spectrometry (MS) approaches can give mixed results as no pure starting material for the biomolecular methods of choice is available. https://www.leica-microsystems.com//science-lab/correlation-of-phenotype-and-genotype-microscopic-samples-and-their-biomolecular-backgrounds/ Thu, 20 Apr 2017 07:08:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/18921 Super-Resolution The Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy Hemidesmosomes have been extensively studied by immunofluorescence microscopy, but due to its limited resolution, their precise organization remained poorly understood. We studied hemidesmosome organization in cultured keratinocytes by 2- and 3-color super-resolution microscopy. We observed that in the cell periphery, nascent hemidesmosomes are associated with individual keratin filaments and that β4 is distributed along rather than under keratin filaments. By applying innovative methods to quantify molecular distances, we demonstrate that the hemidesmosomal plaque protein plectin interacts simultaneously and asymmetrically with β4 and keratin. https://www.leica-microsystems.com//science-lab/the-molecular-architecture-of-hemidesmosomes-as-revealed-by-super-resolution-microscopy/ Fri, 10 Feb 2017 09:11:00 +0000 MSc Leila Nahidiazar https://www.leica-microsystems.com/18278 Super-Resolution Live-Cell Imaging Super-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics Plasma membrane dynamics are an important ruler of cellular activity, particularly through the interaction and diffusion dynamics of membrane-embedded proteins and lipids. FCS (fluorescence correlation spectroscopy) on an optical (confocal) microscope is a popular tool for investigating such dynamics. Unfortunately, its full applicability is constrained by the limited spatial resolution of a conventional optical microscope. The present chapter depicts the combination of optical super-resolution STED (stimulated emission depletion) microscopy with FCS, and why it is an important tool for investigating molecular membrane dynamics in living cells. Compared with conventional FCS, the STED-FCS approach demonstrates an improved possibility to distinguish free from anomalous molecular diffusion, and thus to give new insights into lipid–protein interactions and the traditional lipid ‘raft’ theory. https://www.leica-microsystems.com//science-lab/super-resolution-optical-microscopy-of-lipid-plasma-membrane-dynamics/ Fri, 23 Dec 2016 14:16:00 +0000 Prof. Christian Eggeling https://www.leica-microsystems.com/18885 Fluorescence Microscopy CRISPR-Cas9 Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target sequence via Watson-Crick base pairing. It serves as a marker for the foreign nucleic acid. The second component is the Cas9 protein. It binds to the marked sequence and cuts it due to its nuclease activity. Because the base pairing RNA can be synthesized easily and then used to determine a target region, researchers have utilized this system in the laboratory for genome editing. https://www.leica-microsystems.com//science-lab/gene-editing-with-crisprcas9-breakthrough-in-genome-engineering/ Thu, 17 Nov 2016 18:54:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/18806 Super-Resolution Practical Guide for Excellent GSDIM Super-Resolution Images Do you know that most protists and bacteria lack in one feature that each of our body cell has? Our cells are touch and communicate with one another. They send and receive a variety of signals that coordinate their behavior to act together as a functional multicellular organism. Exploring the way of cellular communication and the ways how the cell surface interacts to organize tissues and body structures is of great interest. Kees Jalink and his team of scientists at the Netherlands Cancer Institute (NKI) in Amsterdam obtained new scientific insights into the molecular architecture of hemidesmosomes, cytoskeletal components, cell surface receptors and vesicular proteins with the help of Ground-State-Depletion (GSD)/ dSTORM microscopy. In this interview, Kees Jalink comments on their developments in imaging chambers, buffer conditions and image analysis to get the perfect super resolution image. https://www.leica-microsystems.com//science-lab/practical-guide-for-excellent-gsdim-super-resolution-images/ Wed, 26 Oct 2016 06:58:00 +0000 PhD Kees Jalink, PhD Tamara Straube, MSc Leila Nahidiazar, MSc Daniela Leyton Puig https://www.leica-microsystems.com/18919 Super-Resolution Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the mechanism of initiation of lamellipodia and ruffles remains poorly understood. Here, we show that mDia1 acts in concert with the Arp2/3 complex to promote initiation of lamellipodia and ruffles. https://www.leica-microsystems.com//science-lab/initiation-of-lamellipodia-and-ruffles-involves-cooperation-between-mdia1-and-the-arp23-complex/ Mon, 24 Oct 2016 11:47:00 +0000 https://www.leica-microsystems.com/18876 Super-Resolution Abstracts of the 6th European Super-Resolution User-Club Meeting The 6th European Super-Resolution User Club Meeting was held in collaboration with Dr. Timo Zimmermann, CRG, and Dr. Pablo Loza-Alvarez, ICFO, Barcelona. According to the founding principle of the club of keeping close to science, both imaging facilities at the CRG and the ICFO opened their doors to the User Club members, allowing them to explore exciting super-resolution and and nanoscopy applications. The meeting agenda covered highly relevant talks around this year’s central theme “Core Facilities and Super-Resolution Microscopy”, as well as plenty of opportunities to network amongst super-resolution users from different European countries. Here we present the abstracts of the talks held during the meeting. https://www.leica-microsystems.com//science-lab/abstracts-of-the-6th-european-super-resolution-user-club-meeting/ Tue, 18 Oct 2016 08:59:00 +0000 Dr. Timo Zimmermann, Dr. Pablo Loza-Alvarez, Dr. Alberto Lleó, Dr. Gražvydas Lukinavicius, Prof. Philip Tinnefeld, Prof. Hans-Georg Kräusslich, Dr. Steffen Dietzel, Dr. Valeria Caiolfa, Lorenzo Albertazzi https://www.leica-microsystems.com/18691 Stereo Microscopy Confocal Microscopy Fluorescence Microscopy Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans For scientists, technicians, and teachers working with the worm C. elegans in the research lab or classroom, this report is intended to give useful information to help improve their daly work. The aim is to make the work steps of worm picking, transgenesis, RNA interference, screening, and functional imaging efficient. It also details the various possibilities for equipping a research worm lab or biology classroom/teaching lab explaining worm methods. https://www.leica-microsystems.com//science-lab/work-efficiently-in-developmental-biology-with-stereo-and-confocal-microscopy-c-elegans/ Mon, 19 Sep 2016 06:09:00 +0000 PhD James DeRose, PhD Heinrich Bürgers, PhD Martin Gamerdinger https://www.leica-microsystems.com/18112 Confocal Microscopy The Apical Ectodermal Ridge of the Mouse Model of Ectrodactyly Dlx5;Dlx6−/− Shows Altered Stratification and Cell Polarity, which are Restored by Exogenous Wnt5a Ligand The congenital malformation split hand/foot (SHFM) is characterized by missing central fingers and dysmorphology or fusion of the remaining ones. During limb development, the apical ectodermal ridge (AER) is a key-signaling center responsible for early proximal–distal growth and patterning. In search for the mechanism, we examined the non-canonical Wnt signaling, considering that Dwnt-5 is a target of distalless in Drosophila and the knockout of Wnt5, Ryk, Ror2 and Vangl2 in the mouse causes severe limb malformations. https://www.leica-microsystems.com//science-lab/the-apical-ectodermal-ridge-of-the-mouse-model-of-ectrodactyly-dlx5dlx6-shows-altered-stratification-and-cell-polarity-which-are-restored-by-exogenous-wnt5a-ligand/ Wed, 07 Sep 2016 07:18:00 +0000 https://www.leica-microsystems.com/13942 Fluorescence Microscopy Quantitative Imaging Video Talk by Roger Tsien: Fluorescent Protein Indicators In this talk, Roger Tsien discusses how fluorescent proteins have been turned into indicators for a wide variety of biological molecules, including pH, ions, redox potential, and signaling molecules like phosphoinositides. The talk also covers reporters used to measure the activity of enzymes like kinases, phosphatases, and proteases. It covers both single proteins whose intensity or wavelength change, as well as reporters using Förster resonance energy transfer (FRET). https://www.leica-microsystems.com//science-lab/video-talk-by-roger-tsien-fluorescent-protein-indicators/ Tue, 16 Aug 2016 10:29:00 +0000 PhD Roger Y. Tsien https://www.leica-microsystems.com/18623 Fluorescence Microscopy CRISPR-Cas9 Video Talk: CRISPR-Cas – From a Bacterial Adaptive Immune System to a Genome Engineering Tool The CRISPR-Cas system was originally discovered as an adaptive immune system of bacteria and archaea to protect against viral attack. During this talk, given at Leica Microsystems in Wetzlar, Dr. Lennart Randau, MPI Marburg, presents this simple and fascinating system in detail. Furthermore, he introduces the adaption of the CRISPR-Cas system into a potent molecular biology tool, which is used heavily for genome editing. In addition to its influence on molecular biology, meanwhile the Cas9 nuclease also stimulates microscopy techniques e.g. to fluorescently label genomic loci in living cells. https://www.leica-microsystems.com//science-lab/video-talk-crispr-cas-from-a-bacterial-adaptive-immune-system-to-a-genome-engineering-tool/ Tue, 12 Jul 2016 04:13:00 +0000 Dr. Lennart Randau https://www.leica-microsystems.com/18271 Super-Resolution Quantitative Imaging Live-Cell Imaging STED-FLCS: An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics Heterogeneous diffusion dynamics of molecules play an important role in many cellular signaling events, such as of lipids in plasma membrane bioactivity. However, these dynamics can often only be visualized by single-molecule and super-resolution optical microscopy techniques. Using fluorescence lifetime correlation spectroscopy (FLCS, an extension of fluorescence correlation spectroscopy, FCS) on a super-resolution stimulated emission depletion (STED) microscope, we here extend previous observations of nanoscale lipid dynamics in the plasma membrane of living mammalian cells. https://www.leica-microsystems.com//science-lab/sted-flcs-an-advanced-tool-to-reveal-spatiotemporal-heterogeneity-of-molecular-membrane-dynamics/ Mon, 11 Jul 2016 08:53:00 +0000 Ph.D. Giuseppe Vicidomini https://www.leica-microsystems.com/18431 Quantitative Imaging Live-Cell Imaging Individual Macromolecule Motion in a Crowded Living Cell There is solid evidence for analyzing fluorescence correlation and dual color fluorescence crosscorrelation spectroscopy data (FCS and dual color FCCS) in cellular applications by equations based on anomalous subdiffusion. Using equations based on normal diffusion causes artifacts of the fitted biological system response parameters and of the interpretations of the FCS and dual color FCCS data in the crowded environment of living cells. Equations based on normal diffusion are not valid in living cells. The original article embraces the status of the experimental situation and touches obstacles that still hinder the applications of single molecules in the cellular environment. https://www.leica-microsystems.com//science-lab/individual-macromolecule-motion-in-a-crowded-living-cell/ Mon, 27 Jun 2016 04:57:00 +0000 https://www.leica-microsystems.com/17977 Confocal Microscopy Live-Cell Imaging The Bimodally Expressed MicroRNA miR‐142 Gates Exit from Pluripotency A stem cell's decision to self‐renew or differentiate is thought to critically depend on signaling cues provided by its environment. It is unclear whether stem cells have the intrinsic capacity to control their responsiveness to environmental signals that can be fluctuating and noisy. Using a novel single‐cell microRNA activity reporter, we show that miR‐142 is bimodally expressed in embryonic stem cells, creating two states indistinguishable by pluripotency markers. https://www.leica-microsystems.com//science-lab/the-bimodally-expressed-microrna-mir-142-gates-exit-from-pluripotency/ Fri, 13 May 2016 13:47:00 +0000 https://www.leica-microsystems.com/16593 Super-Resolution Neuroscience Super-Resolution Microscopy of the Synaptic Active Zone At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Recently, super-resolution microscopy (SRM) techniques have begun to enter the neurosciences. These approaches combine high spatial resolution with the molecular specificity of fluorescence microscopy. Here, we discuss how SRM techniques can be used to obtain information on the organization of AZ proteins. https://www.leica-microsystems.com//science-lab/super-resolution-microscopy-of-the-synaptic-active-zone/ Tue, 15 Dec 2015 10:15:00 +0000 Nadine Ehmann https://www.leica-microsystems.com/16814 Laser Microdissection Neuroscience Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as it allows to separate distinct brain layers or even to isolate individual neurons. https://www.leica-microsystems.com//science-lab/workflows-protocols-connecting-microscopy-and-molecular-biology-in-neuroscience/ Fri, 04 Dec 2015 16:01:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/13935 Fluorescence Microscopy Video Talk by Roger Tsien: Fluorescent Proteins Live cell imaging has been revolutionized by the discovery of the green fluorescent protein (GFP). This lecture covers the history of GFP, how it folds and becomes fluorescent, how it has been mutated to produce additional colors (blue, cyan, yellow), and the discovery of red fluorescent proteins from corals. It also covers novel photoswitchable and photoactivatible fluorescent proteins, whose color can be changed by light, and new infrared fluorescent proteins. https://www.leica-microsystems.com//science-lab/video-talk-by-roger-tsien-fluorescent-proteins/ Wed, 07 Oct 2015 16:55:00 +0000 PhD Roger Y. Tsien https://www.leica-microsystems.com/16019 Super-Resolution Abstracts of the 5th European Super-Resolution User-Club Meeting The 5th Super-Resolution User Club Meeting was held in collaboration with Professor Kees Jalink and The Netherlands Cancer Institute (NKI) in Amsterdam. Having the meeting at a location where super-resolution microscopy is used on a daily basis makes a big difference, offering participants the chance to use live cells for workshops and see systems working in their true environments. Thanks also to the scientists that supported the meeting by coming and giving talks. As super-resolution continues to grow in importance in research, we recognize the need to come together to network, share information and experiences. Here we present the abstracts of the talks. https://www.leica-microsystems.com//science-lab/abstracts-of-the-5th-european-super-resolution-user-club-meeting/ Tue, 23 Jun 2015 13:23:00 +0000 PhD Kees Jalink, PhD Jürgen J. Schmied, PhD Jonas Ries, PhD David Williamson, MSc Daniela Leyton Puig, Robert Nieuwenhuizen, MSc Leila Nahidiazar, PhD Luc Reymond, PhD Imre Gaspar, Luca Lanzanò, Ivan Michel Antolovic https://www.leica-microsystems.com/14492 Fluorescence Microscopy Defect Transport Mechanism Leads to Shortened Chromosome Ends Scientists at the University of Göttingen have deciphered the biogenesis of an enzyme complex whose role is to ensure that the ends of chromosomes are not shortened during the cell division process and that the genetic material is fully maintained. The results were published in Cell Reports magazine. https://www.leica-microsystems.com//science-lab/defect-transport-mechanism-leads-to-shortened-chromosome-ends/ Fri, 26 Sep 2014 16:46:00 +0000 Haijia Wu https://www.leica-microsystems.com/14017 Super-Resolution Image Restoration and Deconvolution Abstracts of the 4th European Super-Resolution User-Club Meeting The 4th Super-Resolution User Club Meeting was held in collaboration with Christian Eggeling and the Weatherall Institute of Molecular Medicine in Oxford, UK. Here we present the abstracts of the talks and interviews with participants. https://www.leica-microsystems.com//science-lab/abstracts-of-the-4th-european-super-resolution-user-club-meeting/ Fri, 08 Aug 2014 08:09:00 +0000 Prof. Christian Eggeling, Ph.D. Giuseppe Vicidomini, MSc Leila Nahidiazar, Dr. Sergi Padilla-Parra, Prof. Mark Neil, Dr. Marko Lampe, PhD Kees Jalink, Dr. Katrin Willig, Dr. Timo Zimmermann, Ph.D. Marc van Zandvoort https://www.leica-microsystems.com/12989 Confocal Microscopy Genomic Survey, Gene Expression Analysis and Structural Modeling Suggest Diverse Roles of DNA Methyltransferases in Legumes DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases), namely Methyltransferase (MET), Chromomethylase (CMT) and Domains Rearranged Methyltransferase (DRM), which maintain methylation at CG, CHG and CHH sites. DNA MTases have not been studied in legumes so far. Here, we report the identification and analysis of putative DNA MTases in five legumes, including chickpea, soybean, pigeonpea, Medicago and Lotus. MTases in legumes could be classified in known MET, CMT, DRM and DNA nucleotide methyltransferases (DNMT2) subfamilies based on their domain organization. https://www.leica-microsystems.com//science-lab/genomic-survey-gene-expression-analysis-and-structural-modeling-suggest-diverse-roles-of-dna-methyltransferases-in-legumes/ Fri, 14 Mar 2014 11:29:00 +0000 https://www.leica-microsystems.com/12768 Neuroscience Confocal Microscopy Multiphoton Microscopy Map the Brain with CLARITY Imaging whole brains with CLARITY and multiphoton microscopy. Image a whole brain without sectioning? Investigate neuronal circuits without reconstruction? Perform molecular phenotyping without destroying subcellular structures? Understanding the brain with molecular resolution and global scope has always been challenging. The novel CLARITY method, developed by the Deisseroth laboratory at Stanford University, USA, pushes the barrier of deep tissue imaging a big step ahead. https://www.leica-microsystems.com//science-lab/map-the-brain-with-clarity/ Tue, 11 Mar 2014 07:26:00 +0000 PhD Isabelle Köster https://www.leica-microsystems.com/12787 Laser Microdissection Sorbin and SH3 Domain‐Containing Protein 2 Is Released from Infarcted Heart in the Very Early Phase: Proteomic Analysis of Cardiac Tissues From Patients Many animal experiments have been performed to date to elucidate the mechanism of cardiac ischemic injury, and these studies have enhanced our understanding of cardiac remodeling following hypoxic stress. Clinical studies using samples from admitted patients have also been conducted to discover diagnostic or prognostic biomarkers for acute myocardial infarction (AMI). https://www.leica-microsystems.com//science-lab/sorbin-and-sh3-domain-containing-protein-2-is-released-from-infarcted-heart-in-the-very-early-phase-proteomic-analysis-of-cardiac-tissues-from-patients/ Wed, 05 Mar 2014 19:11:00 +0000 https://www.leica-microsystems.com/12452 Neuroscience Live-Cell Imaging The 50 Most Influential Scientists in the World Today From neuroscience, biotechnology and digital media to sustainable energy and cloud computing, almost everything today is somehow affected – and sometimes entirely reshaped – by scientific and technological advances. https://www.leica-microsystems.com//science-lab/good-to-know/the-50-most-influential-scientists-in-the-world-today/ Mon, 17 Feb 2014 19:03:00 +0000 https://www.leica-microsystems.com/11038 Confocal Microscopy Live-Cell Imaging Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic On October 7th 2013, The Nobel Assembly at Karolinska Institutet has decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof "for their discoveries of machinery regulating vesicle traffic, a major transport system in our cells". https://www.leica-microsystems.com//science-lab/nobel-prize-2013-in-physiology-or-medicine-for-discoveries-of-the-machinery-regulating-vesicle-traffic/ Fri, 11 Oct 2013 15:14:00 +0000 https://www.leica-microsystems.com/10924 Confocal Microscopy Live-Cell Imaging A Flip of the Mitotic Spindle has Disastrous Consequences for Epithelial Cells Constructing a body is like building a house – if you compromise structural integrity, the edifice can collapse. Nowhere is that clearer on a cellular level than in the case of epithelial sheets, single layers of cells that line every body cavity from the gut to mammary glands. Stowers Institute for Medical Research Associate Investigator Matt Gibson, PhD, and his team use simple animal systems like fruit flies and sea anemones to investigate how epithelial cells maintain order while getting jostled by cell division. https://www.leica-microsystems.com//science-lab/a-flip-of-the-mitotic-spindle-has-disastrous-consequences-for-epithelial-cells/ Fri, 04 Oct 2013 07:58:00 +0000 PhD Yu-ichiro Nakajima, PhD Matthew C. Gibson https://www.leica-microsystems.com/10826 Super-Resolution STED Microscopy: A New Chapter in Light Imaging When Stefan Hell invented a technique that pushed beyond the diffraction limit of light for the first time, a new field of nanoscale imaging was born. Now laboratories around the world are using STED microscopy to smash through more boundaries in fundamental science. https://www.leica-microsystems.com//science-lab/sted-microscopy-a-new-chapter-in-light-imaging/ Mon, 16 Sep 2013 10:28:00 +0000 https://www.leica-microsystems.com/10653 Super-Resolution Live-Cell Imaging Watching Molecule Movements in Live Cells The newly developed STED-RICS microscopy method records rapid movements of molecules in live samples. By combining raster image correlation spectroscopy (RICS) with STED fluorescence microscopy, researchers of Karlsruhe Institute of Technology (KIT) opened up new applications in medical research, e.g. analyzing the dynamics of cell membranes at high protein concentrations. https://www.leica-microsystems.com//science-lab/watching-molecule-movements-in-live-cells/ Thu, 22 Aug 2013 13:28:00 +0000 https://www.leica-microsystems.com/10135 Super-Resolution Widefield Microscopy Image Restoration and Deconvolution Abstracts of the 3rd European Super-Resolution User-Club Meeting The 3rd meeting of the Leica Super-Resolution User Club was held from June 17th to 19th, 2013 in collaboration with Alberto Diaspro and the Italian Institute of Technology (IIT) in Genoa. Confocal and widefield super-resolution users from ten European countries took three days’ out to deepen their knowledge on super-resolution techniques and applications and make use of an opportunity for full exchange of experiences. https://www.leica-microsystems.com//science-lab/abstracts-of-the-3rd-european-super-resolution-user-club-meeting/ Mon, 08 Jul 2013 10:27:00 +0000 Prof. Alberto Diaspro, Ph.D. Illaria Testa, Prof. Silvio Rizzoli, Ph.D. Giuseppe Vicidomini, Prof. Ralf Jacob, Ph.D. Eric Hosy, Prof. Colin Sheppard, Zeno Lavagnino https://www.leica-microsystems.com/9759 Quantitative Imaging Step by Step Guide for FRAP Experiments Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be used to determine kinetic properties, like the diffusion coefficient, mobile fraction, and transport rate of the fluorescently labeled molecules. FRAP employs irradiation of a fluorophore with a short laser pulse. State of the art laser scanning microscopes, like the TCS SP8 confocal system, have the advantage of using a high intensity laser for photobleaching and a low intensity laser for image recording. With the LAS AF application wizard you can choose between different ways to carry out a FRAP experiment. https://www.leica-microsystems.com//science-lab/step-by-step-guide-for-frap-experiments/ Fri, 17 May 2013 13:50:00 +0000 Dr. Jan Schröder https://www.leica-microsystems.com/9492 Live-Cell Imaging Fluorescence Microscopy Quantitative Imaging Imaging Enzymes at Work For the understanding of functions of proteins in biological and pathological processes, reporter molecules such as fluorescent proteins have become indispensable tools for visualizing the location of these proteins in intact animals, tissues, and cells. For enzymes, imaging their activity also provides information on their function or functions, which does not necessarily correlate with their location. Metabolic mapping enables imaging of activity of enzymes. https://www.leica-microsystems.com//science-lab/imaging-enzymes-at-work/ Fri, 05 Apr 2013 14:27:00 +0000 https://www.leica-microsystems.com/9495 Basics in Microscopy Image Cytometry Microscopy-based imaging is booming and the need for tools to retrieve quantitative data from images is urgent. This book provides simple but reliable tools to generate valid quantitative gene expression data, at the mRNA, protein and activity level, from microscopic images in relation to structures in cells, tissues and organs in 2D and 3D. https://www.leica-microsystems.com//science-lab/image-cytometry/ Thu, 04 Apr 2013 20:08:00 +0000 https://www.leica-microsystems.com/7401 EM Sample Preparation Capturing Neurotransmitter Receptors and Ion Channels Neurotransmitter receptors and ion channels in the central nervous system are localized to synaptic and extrasynaptic membrane compartments of pre- and postsynaptic elements of neurons. The impact of the activation of these proteins on synaptic integration and regulation of transmitter release depends on their precise location relative to synapses, as well as on the density and coupling of molecules in microcompartments of the cells. High-resolution qualitative and quantitative visualization of membranebound receptors and ion channels is, therefore, essential for understanding their roles in cell communication. https://www.leica-microsystems.com//science-lab/capturing-neurotransmitter-receptors-and-ion-channels/ Thu, 14 Feb 2013 23:00:00 +0000 Daniel Althof, Akos Kulik https://www.leica-microsystems.com/8109 Confocal Microscopy Stereo Microscopy Live-Cell Imaging Molecular Developmental Biology: Norwegian Marine Research Scientists Solve the Mysteries of Evolution The human nervous system is an infinitely complex network consisting of some 100 billions of neurons. It is the result of many-faceted evolutionary processes spanning millions of years which, like the development of other organ systems, have been little researched so far. https://www.leica-microsystems.com//science-lab/molecular-developmental-biology-norwegian-marine-research-scientists-solve-the-mysteries-of-evolution/ Mon, 11 Feb 2013 23:00:00 +0000 M. A. Janika Wiesner, Dr. Andreas Hejnol https://www.leica-microsystems.com/7275 Laser Microdissection Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome" The physiological process of aging is an elementary part of life. The processes responsible for the aging process and the effect they have is one of the key focuses of biomedical research. Dr. Yvonne Begus-Nahrmann researches molecular mechanisms in stem cells and their role in aging and tumor development at the Göttingen University Medical Center. Signal proteins such as p53 and p21 appear to play an important role in this context. To investigate these proteins in specific cell types, the cells are isolated from organ tissue with the aid of laser microdissection. https://www.leica-microsystems.com//science-lab/stem-cells-in-the-aging-process-the-signal-protein-p53-as-guardian-of-the-genome/ Thu, 08 Nov 2012 23:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille, Dr. Yvonne Begus-Nahrmann https://www.leica-microsystems.com/7735 Super-Resolution Widefield Microscopy Image Restoration and Deconvolution Abstracts of the 2nd European Super-Resolution User-Club Meeting The 2nd meeting of the Leica Super-resolution User club was held from September 25 to 27, 2012 in collaboration with the Science for Life Laboratory at the Karolinska Institute, Stockholm, Sweden. With a mixture of engaging talks by key experts in the field of super-resolution microscopy and stimulating discussion sessions, the meeting proved as popular as last year’s event, attracting a wide range of scientists interested in both confocal and widefield super-resolution and sample preparation techniques. https://www.leica-microsystems.com//science-lab/abstracts-of-the-2nd-european-super-resolution-user-club-meeting/ Thu, 01 Nov 2012 23:00:00 +0000 Prof. Hjalmar Brismar, Prof. Dr. Dr. h.c. Stefan Hell, Prof. Christian Eggeling, Dr. Anna Szymborska, Prof. Alberto Diaspro, Ph.D. Eric Hosy, Ph.D. Giuseppe Vicidomini, Hans van der Voort https://www.leica-microsystems.com/7364 Confocal Microscopy Live-Cell Imaging Nobel Prize 2012 in Chemistry for Studies of G-Protein-Coupled Receptors The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in Chemistry for 2012 to Robert J. Lefkowitz and Brian K. Kobilka for studies of G-protein–coupled receptors. https://www.leica-microsystems.com//science-lab/nobel-prize-2012-in-chemistry-for-studies-of-g-protein-coupled-receptors/ Thu, 11 Oct 2012 22:00:00 +0000 Nobel Media AB 2012 https://www.leica-microsystems.com/6792 Live-Cell Imaging Stereo Microscopy Taking the Long View In exploring how embryos take shape, John Wallingford has identified a key pathway involved in vertebrate development – and human disease. https://www.leica-microsystems.com//science-lab/taking-the-long-view/ Mon, 10 Sep 2012 22:00:00 +0000 https://www.leica-microsystems.com/5217 Fluorescence Microscopy Live-Cell Imaging Neuroscience TIRF Microscopy Confocal Microscopy Quantitative Imaging The New Repository on the Block The need for data validation and accessibility has never been greater than it is today. We are inundated with information from a multitude of resources, but how can we easily evaluate the accuracy of that data? In the past, the peer review process provided this and was often run by publishers. https://www.leica-microsystems.com//science-lab/the-new-repository-on-the-block/ Mon, 06 Feb 2012 23:00:00 +0000 M.B.A. David N. Orloff, Ph.D. Janet Iwasa, Ph.D. Caroline Kane https://www.leica-microsystems.com/10866 CLEM Fluorescence Microscopy EM Sample Preparation A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues. Here we introduce ‘"miniSOG"’ (for mini Singlet Oxygen Generator), a fluorescent flavoprotein engineered from Arabidopsis phototropin 2. https://www.leica-microsystems.com//science-lab/a-genetically-encoded-tag-for-correlated-light-and-electron-microscopy-of-intact-cells-tissues-and-organisms/ Sun, 04 Sep 2011 19:37:00 +0000 https://www.leica-microsystems.com/3819 Laser Microdissection An Introduction to Laser Microdissection Laser microdissection is a highly selective process for preparing samples for DNA, RNA and protein analysis. It is a microscope-controlled manipulation technique for the precise separation of samples and tissue using a focused laser beam. https://www.leica-microsystems.com//science-lab/an-introduction-to-laser-microdissection/ Tue, 28 Jun 2011 15:39:57 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/3835 Laser Microdissection Applications of Laser Microdissection Laser microdissection and laser micromanipulation are suitable for gaining a differentiated insight into the function of genes and proteins, and are used for a wide range of applications in neurobiology and immunology as well as in the developmental and cell biology of animal and plant organisms. https://www.leica-microsystems.com//science-lab/applications-of-laser-microdissection/ Sun, 26 Jun 2011 22:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/9352 Quantitative Imaging Confocal Microscopy FRET Measurements on Fuzzy Fluorescent Nanostructures In the last decade, fluorescence resonance energy transfer (FRET) has become a useful technique for studying intermolecular interactions applied to the analysis of biological systems. Although FRET measurements may be very helpful in the comprehension of different cellular processes, it can be difficult to obtain quantitative results, hence the necessity of studying FRET on controllable systems. https://www.leica-microsystems.com//science-lab/fret-measurements-on-fuzzy-fluorescent-nanostructures/ Tue, 27 Mar 2007 22:00:00 +0000 Valentina Caorsi, Emiliano Ronzitti, Ph.D. Giuseppe Vicidomini, Prof. Gail McConnell, Prof. Alberto Diaspro