Leica Science Lab - Tag : Neuroscience https://www.leica-microsystems.com//science-lab/tag/tags/neuroscience/show/Tag/ Article tagged with Neuroscience en-US https://www.leica-microsystems.com/3815 Laser Microdissection Neuroscience Forensics Laser Microdissection Publication List This list presents current publications in the major application fields for laser microdissection and is updated monthly. https://www.leica-microsystems.com//science-lab/laser-microdissection-publication-list/ Wed, 25 Mar 2020 07:57:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/28066 Widefield Microscopy Evaluating Axon Regeneration After Brain or Spine Trauma of Mice Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and non-active axons using widefield and THUNDER imaging technology. Results indicated a better discrimination between active and non-active axons in THUNDER images. https://www.leica-microsystems.com//science-lab/evaluating-axon-regeneration-after-brain-or-spine-trauma-of-mice/ Tue, 17 Mar 2020 11:55:00 +0000 PhD James DeRose, Robert Fasulka https://www.leica-microsystems.com/27780 Surgical Microscopy GLOW800 Augmented Reality Fluorescence in Aneurysm Treatment This case study from Prof. Dr. Feres Chaddad talks about the treatment of unruptured MCA (middle cerebral artery) and PCOM (posterior communicating artery) aneurysms with microsurgical clipping. It illustrates how the Augmented Reality Fluorescence GLOW800 can help surgeons before and after clipping with one augmented, real-time view of the cerebral anatomy, augmented by real-time vascular flow. https://www.leica-microsystems.com//science-lab/glow800-augmented-reality-fluorescence-in-aneurysm-treatment/ Mon, 03 Feb 2020 12:46:00 +0000 Prof Dr. Feres Chaddad, MD Robert Ibe https://www.leica-microsystems.com/4421 Coherent Raman Scattering (CRS) Coherent Raman Scattering Microscopy Publication List CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their molecules. The two most important CRS techniques are Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). The biochemical image contrast of CRS is in many ways complementary to the molecular contrast obtained in fluorescence microscopy. A second crucial advantage of these methods is that they preserve the specimen/sample in a near pristine state. This reference list presents current and basic papers on CRS microscopy. https://www.leica-microsystems.com//science-lab/cars-publication-list/ Tue, 08 Oct 2019 22:00:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/26318 Neuroscience Confocal Microscopy Cellular Motility: Microtubules, Motor Proteins and Tau-Proteins Cellular motility is based on motor-proteins that can bind to filamentous scaffold proteins and – under consumption of ATP – can “crawl” on these filaments. This note is about proteins connected to microtubules, one of the filamentous structures that compose the cytoskeleton. Microtubules are hollow tubes of ca 25nm, composed of tubulin-heterodimers. The proteins are polymerized in a directed fashion, allowing to differentiate a plus-end and a minus-end of the fiber. Another important scaffold component that is also involved in movements, are actin fibers that cooperate with myosin as a motor-protein. The best known movement involving the actin-myosin system is muscular contraction. https://www.leica-microsystems.com//science-lab/cellular-motility-microtubules-motor-proteins-and-tau-proteins/ Mon, 12 Aug 2019 12:34:00 +0000 PhD Jen-Yi Lee, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/25022 EM Sample Preparation Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in which structures are constantly changing. Exploring specific time points of a dynamic process is therefore a major challenge. Exploring a process at the nanoscale through optogenetics or electrical field stimulation in combination with timed millisecond precision vitrification is a promising technology to overcome this challenge. In the first part of a series of application notes the practical considerations of stimulation-assisted vitrification are discussed. https://www.leica-microsystems.com//science-lab/bridging-structure-and-dynamics-at-the-nanoscale-through-optogenetics-and-electrical-stimulation/ Mon, 20 May 2019 22:00:00 +0000 Dr. Andres Kaech, PhD Frédéric Leroux https://www.leica-microsystems.com/27638 Laser Microdissection How to improve your RNA Analysis Workflow with Laser Microdissection Brain research: Single neuron excision for RNA analysis - Improve your workflow with laser microdissection - Parkinson’s disease is a common progressive neurodegenerative disorder. It is connected with cell death of dopamine-releasing neurons in the substantia nigra. Differences in gene expression patterns between individual dopamine-releasing neurons of disease affected and healthy individuals allow defining target genes for therapies. For RNA analysis, single cell resolution is crucial, as investigating the whole tissue is meaningless. Analyzing mixtures of dopamine-releasing neurons and all other brain cells distorts the result. https://www.leica-microsystems.com//science-lab/how-to-improve-your-rna-analysis-workflow-with-laser-microdissection/ Sun, 17 Mar 2019 10:30:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/27643 Laser Microdissection How to improve your Alzheimer Protein Analysis with Laser Microdissection Brain Research: Collect pure starting material for proteomics - Improve your workflow with Laser Microdissection - Many brain diseases result from protein malfunction, misfolding and agglutination. For this reason protein analysis is the key to understanding causes of, and discovering therapies for, many cerebral defects. https://www.leica-microsystems.com//science-lab/how-to-improve-your-alzheimer-protein-analysis-with-laser-microdissection/ Sat, 02 Mar 2019 10:53:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/20202 Super-Resolution Researchers Find a “Digital” Mechanism Behind Neuronal Changes from Learning Neurons react to learning and memory by activating synaptic connections. The mechanisms behind this fundamental process are complex and poorly understood. Researchers at Thomas Jefferson University have found that neuron plasticity operates in a “digital” fashion through nanomodules of discrete size that multiply and strengthen neuronal connections upon stimulation. This breakthrough was published on April 23rd in the journal Nature Neuroscience. https://www.leica-microsystems.com//science-lab/researchers-find-a-digital-mechanism-behind-neuronal-changes-from-learning/ Mon, 04 Jun 2018 22:00:00 +0000 Dr. Martin Hruska, Dr. Julia Roberti https://www.leica-microsystems.com/19607 EM Sample Preparation Interview with Dr. Shigeki Watanabe on Research in Synaptic Membrane Dynamics Dr. Shigeki Watanabe, principle investigator of the department of Cell Biology at the Johns Hopkins University School of Medicine in Baltimore, held a workshop in Zürich, Switzerland on methods to study synaptic dynamics with millisecond precision. In collaboration with Dr. Andres Käch from the University of Zurich all workshop attendees enjoyed presentations and hands-on sessions on the EM ICE by Leica Microsystems with Light and Electrical Stimulation, revealing the latest developments in brain research. During this workshop Dr. Bernd Sägmüller from Leica Microsystems had the chance for an interview with Dr. Watanabe. https://www.leica-microsystems.com//science-lab/interview-with-dr-shigeki-watanabe-on-research-in-synaptic-membrane-dynamics/ Thu, 06 Jul 2017 23:00:00 +0000 Dr. Bernd Sägmüller, PhD Shigeki Watanabe https://www.leica-microsystems.com/18905 Super-Resolution Neuroscience Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been challenged, suggesting that their recycling ability may differ greatly. Botulinum neurotoxin type-A (BoNT/A) is a highly potent neurotoxin that is internalized in synaptic vesicles at motor nerve terminals and induces flaccid paralysis. Recently, BoNT/A was also shown to undergo retrograde transport, suggesting it might enter a specific pool of synaptic vesicles with a retrograde trafficking fate. Using high-resolution microscopy techniques including electron microscopy and single molecule imaging, we found that the BoNT/A binding domain is internalized within a subset of vesicles that only partially co-localize with cholera toxin B-subunit and have markedly reduced VAMP2 immunoreactivity. https://www.leica-microsystems.com//science-lab/botulinum-neurotoxin-type-a-enters-a-non-recycling-pool-of-synaptic-vesicles/ Wed, 19 Oct 2016 11:51:00 +0000 https://www.leica-microsystems.com/18691 Stereo Microscopy Confocal Microscopy Fluorescence Microscopy Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans For scientists, technicians, and teachers working with the worm C. elegans in the research lab or classroom, this report is intended to give useful information to help improve their daly work. The aim is to make the work steps of worm picking, transgenesis, RNA interference, screening, and functional imaging efficient. It also details the various possibilities for equipping a research worm lab or biology classroom/teaching lab explaining worm methods. https://www.leica-microsystems.com//science-lab/work-efficiently-in-developmental-biology-with-stereo-and-confocal-microscopy-c-elegans/ Mon, 19 Sep 2016 06:09:00 +0000 PhD James DeRose, PhD Heinrich Bürgers, PhD Martin Gamerdinger https://www.leica-microsystems.com/18301 EM Sample Preparation Freeze-Fracture Replication of Pyramidal Cells Application Note for Leica EM HPM100 - Frozen samples (90 μm thick slices frozen by HPM100) were inserted into a double replica table and then fractured into two pieces at –130°C (after insertion of the tissue into BAF 060 the samples should be left in the chamber for 20 min to reach the –130°C). https://www.leica-microsystems.com//science-lab/freeze-fracture-replication-of-pyramidal-cells/ Thu, 08 Sep 2016 16:23:00 +0000 Akos Kulik https://www.leica-microsystems.com/18146 EM Sample Preparation Cultured Rat Hippocampal Neurons Application Note for Leica EM ICE - Rat Hippocampal neurons, cultured on 50 μm thick Aclar (Aclar embedding film, EMS) for 19 days, were frozen in the 100 μm deep side of lecithin coated (detailed protocol Appendix I) type A 3 mm Cu/Au carriers (Leica) and sandwiched with the flat side of lecithin coated type B 3 mm Cu/Au carriers (Leica). No additional filler was used, only cell culture medium with the addition of Hepes buffer pH 7.2 to a final concentration of 25 mM. Samples were frozen in a high-pressure freezer (Leica EM ICE). https://www.leica-microsystems.com//science-lab/cultured-rat-hippocampal-neurons/ Mon, 29 Aug 2016 07:12:00 +0000 E. G. van Donselaar, Dr. Martin Harterink, Drs. C. E. M. Vocking, Dr. W. H. Mueller, Prof. Dr. C. C. Hoogenraad https://www.leica-microsystems.com/15645 Confocal Microscopy Live-Cell Imaging Label-free in vivo Imaging of Myelinated Axons in Health and Disease with Spectral Confocal Reflectance Microscopy We report a new technique for high-resolution in vivo imaging of myelinated axons in the brain, spinal cord and peripheral nerve that requires no fluorescent labeling. This method, based on spectral confocal reflectance microscopy (SCoRe), uses a conventional laser scanning confocal system to generate images by merging the simultaneously reflected signals from multiple lasers of different wavelengths. https://www.leica-microsystems.com//science-lab/label-free-in-vivo-imaging-of-myelinated-axons-in-health-and-disease-with-spectral-confocal-reflectance-microscopy/ Fri, 01 Jul 2016 09:29:00 +0000 https://www.leica-microsystems.com/16593 Super-Resolution Neuroscience Super-Resolution Microscopy of the Synaptic Active Zone At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Recently, super-resolution microscopy (SRM) techniques have begun to enter the neurosciences. These approaches combine high spatial resolution with the molecular specificity of fluorescence microscopy. Here, we discuss how SRM techniques can be used to obtain information on the organization of AZ proteins. https://www.leica-microsystems.com//science-lab/super-resolution-microscopy-of-the-synaptic-active-zone/ Tue, 15 Dec 2015 10:15:00 +0000 Nadine Ehmann https://www.leica-microsystems.com/14217 Super-Resolution Neuroscience JC Virus Inclusions in Progressive Multifocal Leukoencephalopathy: Scaffolding Promyelocytic Leukemia Nuclear Bodies Grow With Cell Cycle Transition Through an S-to-G2–Like State in Enlarging Oligodendrocyte Nuclei In progressive multifocal leukoencephalopathy, JC virus–infected oligodendroglia display 2 distinct patterns of intranuclear viral inclusions: full inclusions in which progeny virions are present throughout enlarged nuclei and dot-shaped inclusions in which virions are clustered in subnuclear domains termed “promyelocytic leukemia nuclear bodies” (PML-NBs). Promyelocytic leukemia nuclear bodies may serve a scaffolding role in viral progeny production. https://www.leica-microsystems.com//science-lab/jc-virus-inclusions-in-progressive-multifocal-leukoencephalopathy-scaffolding-promyelocytic-leukemia-nuclear-bodies-grow-with-cell-cycle-transition-through-an-s-to-g2-like-state-in-enlarging-oligodendrocyte-nuclei/ Thu, 18 Dec 2014 13:31:00 +0000 https://www.leica-microsystems.com/14727 Confocal Microscopy Multiphoton Microscopy Deeper Insights in Transparent Animals CLARITY clearing derivatives for multiphoton microscopy. Transparent organisms help us to identify spatial arrangements and connections of cells and tissues, especially neuronal circuits can easily be identified and characterized. CLARITY is on everyone's lips. https://www.leica-microsystems.com//science-lab/deeper-insights-in-transparent-animals/ Mon, 10 Nov 2014 09:48:00 +0000 BS, PhD Viviana Gradinaru, PhD Isabelle Köster https://www.leica-microsystems.com/14658 Super-Resolution Neuroscience A New Probe for Super-Resolution Imaging of Membranes Elucidates Trafficking Pathways The molecular composition of the organelles involved in membrane recycling is difficult to establish as a result of the absence of suitable labeling tools. We introduce in this paper a novel probe, named membrane-binding fluorophore-cysteine-lysine-palmitoyl group (mCLING), which labels the plasma membrane and is taken up during endocytosis. https://www.leica-microsystems.com//science-lab/a-new-probe-for-super-resolution-imaging-of-membranes-elucidates-trafficking-pathways/ Fri, 17 Oct 2014 12:16:00 +0000 https://www.leica-microsystems.com/14402 Multiphoton Microscopy Neuroscience Clearing Procedures for Deep Tissue Imaging Multi-channel multiphoton microscopy with dedicated optics for CLARITY. Why clearing? Curiosity is human nature. And nothing attracts as much curiosity as the inside of living organisms. While in ancient times those who cut human bodies open to do research were put to death, and modern anatomy started only after Pope Clement VII allowed dissection, we can now watch brains working in living animals – and have a good chance of soon being able to interfere with the observed activities for healing (or control) purposes. https://www.leica-microsystems.com//science-lab/clearing-procedures-for-deep-tissue-imaging/ Thu, 18 Sep 2014 12:36:00 +0000 Dr. Rolf T. Borlinghaus, Dr. Andrea Mülter https://www.leica-microsystems.com/14017 Super-Resolution Abstracts of the 4th European Super-Resolution User-Club Meeting The 4th Super-Resolution User Club Meeting was held in collaboration with Christian Eggeling and the Weatherall Institute of Molecular Medicine in Oxford, UK. Here we present the abstracts of the talks and interviews with participants. https://www.leica-microsystems.com//science-lab/abstracts-of-the-4th-european-super-resolution-user-club-meeting/ Fri, 08 Aug 2014 08:09:00 +0000 Prof. Christian Eggeling, Ph.D. Giuseppe Vicidomini, MSc Leila Nahidiazar, Dr. Sergi Padilla-Parra, Prof. Mark Neil, Dr. Marko Lampe, PhD Kees Jalink, Dr. Katrin Willig, Dr. Timo Zimmermann, Ph.D. Marc van Zandvoort https://www.leica-microsystems.com/12777 Neuroscience Video: The Ascent – a Brief History of the Brain This video features a few of the major scientists and findings that have contributed to modern neuroscience: The history of our knowledge of the brain, how technical and conceptual advances led to new insights and the human side of neuroscience through quotations from key individuals on the nature of the brain and consciousness. https://www.leica-microsystems.com//science-lab/video-the-ascent-a-brief-history-of-the-brain/ Thu, 31 Jul 2014 06:04:00 +0000 https://www.leica-microsystems.com/2469 Laser Microdissection Neuroscience The Morbus Parkinson Puzzle A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this disease have been identified. In the case of the genetic familial form, for example, it has been possible to identify various genes that have a causal influence for M. Parkinson. https://www.leica-microsystems.com//science-lab/the-morbus-parkinson-puzzle/ Thu, 27 Mar 2014 11:11:00 +0000 Dr. Falk Schlaudraff, Dr. Olaf Spörkel https://www.leica-microsystems.com/12768 Neuroscience Confocal Microscopy Multiphoton Microscopy Map the Brain with CLARITY Imaging whole brains with CLARITY and multiphoton microscopy. Image a whole brain without sectioning? Investigate neuronal circuits without reconstruction? Perform molecular phenotyping without destroying subcellular structures? Understanding the brain with molecular resolution and global scope has always been challenging. The novel CLARITY method, developed by the Deisseroth laboratory at Stanford University, USA, pushes the barrier of deep tissue imaging a big step ahead. https://www.leica-microsystems.com//science-lab/map-the-brain-with-clarity/ Tue, 11 Mar 2014 07:26:00 +0000 PhD Isabelle Köster https://www.leica-microsystems.com/12772 Neuroscience Optogenetic Toolkit Goes Multicolor Optogenetics is a technique that allows scientists to control neurons’ electrical activity with light by engineering them to express light-sensitive proteins. Within the past decade, it has become a very powerful tool for discovering the functions of different types of cells in the brain. https://www.leica-microsystems.com//science-lab/optogenetic-toolkit-goes-multicolor/ Tue, 25 Feb 2014 12:35:00 +0000 Anne Trafton https://www.leica-microsystems.com/12452 Neuroscience Live-Cell Imaging The 50 Most Influential Scientists in the World Today From neuroscience, biotechnology and digital media to sustainable energy and cloud computing, almost everything today is somehow affected – and sometimes entirely reshaped – by scientific and technological advances. https://www.leica-microsystems.com//science-lab/good-to-know/the-50-most-influential-scientists-in-the-world-today/ Mon, 17 Feb 2014 19:03:00 +0000 https://www.leica-microsystems.com/11033 Neuroscience Stereo Microscopy Live-Cell Imaging Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells Mauthner cells (M-cells) are large reticulospinal neurons located in the hindbrain of teleost fish. They are key neurons involved in a characteristic behavior known as the C-start or escape response that occurs when the organism perceives a threat. The M-cell has been extensively studied in adult goldfish where it has been shown to receive a wide range of excitatory, inhibitory and neuromodulatory signals. We have been examining M-cell activity in embryonic zebrafish in order to study aspects of synaptic development in a vertebrate preparation. In the late 1990s Ali and colleagues developed a preparation for patch clamp recording from M-cells in zebrafish embryos, in which the CNS was largely intact. https://www.leica-microsystems.com//science-lab/patch-clamp-recordings-from-embryonic-zebrafish-mauthner-cells/ Fri, 11 Oct 2013 21:40:00 +0000 https://www.leica-microsystems.com/10290 Super-Resolution Live-Cell Imaging Neuroscience New Labeling Tools Can Help to Realize the Full Potential of Super-Resolution Microscopy Since super-resolution microscopy techniques revolutionized the concept of light microscopy by overcoming the physical diffraction limit, STED microscopy and other super-resolution techniques have aroused considerable interest. The diffraction limit imposes no more constraints on resolution. New microscopes with ever-decreasing resolution limits are being developed, for instance by the inventor of STED microscopy, Prof. Stefan Hell, now director at the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany. https://www.leica-microsystems.com//science-lab/new-labeling-tools-can-help-to-realize-the-full-potential-of-super-resolution-microscopy/ Fri, 02 Aug 2013 10:55:00 +0000 Dr. Matthias Schauen, Dr. Felipe Opazo, Prof. Silvio Rizzoli https://www.leica-microsystems.com/5222 Neuroscience Optogenetics Optogenetics is a technique that allows light-controlled responses of transfected cells. The cells are genetically modified by introduction of genes that code for light-induced channels or ion pumps. The term optogenetics denotes the light control feature introduced by genetic engineering. https://www.leica-microsystems.com//science-lab/optogenetics/ Mon, 29 Oct 2012 12:51:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/5331 Neuroscience Super-Resolution Sharp Live Images from the Mouse Brain To explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell’s team of researchers at the Max Planck Institute for Biophysical Chemistry in Göttingen has made a significant step closer to this goal. Using the STED microscopy developed by Hell, the scientists have, for the first time, managed to record detailed live images inside the brain of a living mouse. https://www.leica-microsystems.com//science-lab/sharp-live-images-from-the-mouse-brain/ Sun, 04 Mar 2012 23:00:00 +0000 Dr. Sebastian Berning, Dr. Katrin Willig, Dr. Heinz Steffens, Dr. Payam Dibaj, Prof. Dr. Dr. h.c. Stefan Hell https://www.leica-microsystems.com/5268 Laser Microdissection Neuroscience Region-Specific Gene Expression in Adult Mouse CNS Tissues Different areas of the Central Nervous System (CNS) display a specific and selective gene expression profile. Here, we used the Laser Microdissection system Leica LMD6500 to study region-specific mRNA expression in the adult mouse retina and hippocampus. https://www.leica-microsystems.com//science-lab/region-specific-gene-expression-in-adult-mouse-cns-tissues/ Tue, 21 Feb 2012 23:00:00 +0000 Andrea Messina, Mark Dunleavy, Ph.D. Paola Sgadò, Valentina Adami, Ph.D. Yuri Bozzi, Ph.D. Simona Casarosa https://www.leica-microsystems.com/4849 Laser Microdissection Neuroscience TED Talk of Allan Jones: Mapping the Human Brain How can we begin to understand the complexity of the human brain and the way it works? The same way we begin to understand a city: by making a map. In this talk, Allan Jones shows how his team is mapping which genes are turned on in each tiny region, and how it all connects up. https://www.leica-microsystems.com//science-lab/ted-talk-of-allan-jones-mapping-the-human-brain/ Fri, 02 Dec 2011 16:42:00 +0000 Ph.D. Allan Jones https://www.leica-microsystems.com/10165 Super-Resolution Neuroscience Live-Cell Imaging STED Nanoscopy of Actin Dynamics in Synapses deep inside Living Brain Slices It is difficult to investigate the mechanisms that mediate long-term changes in synapse function because synapses are small and deeply embedded inside brain tissue. Although recent fluorescence nanoscopy techniques afford improved resolution, they have so far been restricted to dissociated cells or tissue surfaces. However, to study synapses under realistic conditions, one must image several cell layers deep inside more-intact, three-dimensional preparations that exhibit strong light scattering, such as brain slices or brains in vivo. https://www.leica-microsystems.com//science-lab/sted-nanoscopy-of-actin-dynamics-in-synapses-deep-inside-living-brain-slices/ Wed, 07 Sep 2011 16:39:00 +0000 https://www.leica-microsystems.com/9689 Neuroscience Confocal Microscopy Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further and currently there are many different ways to prepare organotypic cultures. The method presented here was adapted from the one described by Stoppini et al. for the preparation of the slices and from Gogolla et al. for the staining procedure. https://www.leica-microsystems.com//science-lab/organotypic-cerebellar-cultures-apoptotic-challenges-and-detection/ Tue, 17 May 2011 17:50:00 +0000 https://www.leica-microsystems.com/3862 Laser Microdissection Neuroscience Users Report on the Relevance of Laser Microdissection for Their Research Results Laser dissection is used in a large number of research fields, e.g. neurology, cancer research, plant analysis. Here, user report on the research results they have attained by using laser microdissection. https://www.leica-microsystems.com//science-lab/users-report-on-the-relevance-of-laser-microdissection-for-their-research-results/ Tue, 12 Apr 2011 22:00:00 +0000 https://www.leica-microsystems.com/3000 Live-Cell Imaging Neuroscience Mapping Billions of Synapses with Microscopy and Mathematics A combination of widefield imaging techniques and image segmentation analysis enable researchers to map learning-induced functional changes in individual synapses throughout the hippocampus. https://www.leica-microsystems.com//science-lab/mapping-billions-of-synapses-with-microscopy-and-mathematics/ Tue, 12 Apr 2011 22:00:00 +0000 Dr. Christopher S. Rex, Allison Paradise https://www.leica-microsystems.com/2471 Neuroscience Super-Resolution The Missing Link to the Nanocosm of Life Fully understanding the functionality and complexity of the human central nervous system remains one of the major open questions in modern science. Stimulated emission depletion microscopy (STED) can be the method to reveal biological nanostructures https://www.leica-microsystems.com//science-lab/the-missing-link-to-the-nanocosm-of-life/ Mon, 01 Nov 2010 23:00:00 +0000 https://www.leica-microsystems.com/2727 Neuroscience Super-Resolution Restless Receptors Synapses are the switch-points in our brain for information transmission, learning and memory. News studies and developments of imaging techniques have provided new insights into the dynamics of glutamate receptors. The use of superresolution technologies is making an essential contribution to this research. https://www.leica-microsystems.com//science-lab/restless-receptors/ Mon, 01 Nov 2010 23:00:00 +0000 Dipl. oec.-troph. Anja Schué, PhD Daniel Choquet https://www.leica-microsystems.com/2732 Confocal Microscopy Multiphoton Microscopy Live-Cell Imaging Neuroscience Exploring the Concert of Neuronal Activities Brain research using Confocal and Multiphoton Microscopy. Using imaging techniques such as confocal and two-photon microscopy, neuronal dendritic arborization of neurons and their synaptic interconnections can be visualized. https://www.leica-microsystems.com//science-lab/exploring-the-concert-of-neuronal-activities/ Mon, 01 Nov 2010 23:00:00 +0000 Ph.D. Randy Bruno, Ph.D. Myriam Gastard https://www.leica-microsystems.com/9687 Neuroscience Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells We will demonstrate how to study the functional effects of introducing a point mutation in an ion channel. We study G protein-gated inwardly rectifying potassium (referred to as GIRK) channels, which are important for regulating the excitability of neurons. There are four different mammalian GIRK channel subunits (GIRK1-GIRK4) – we focus on GIRK2 because it forms a homotetramer. https://www.leica-microsystems.com//science-lab/mutagenesis-and-functional-analysis-of-ion-channels-heterologously-expressed-in-mammalian-cells/ Fri, 01 Oct 2010 16:35:00 +0000 https://www.leica-microsystems.com/10214 Super-Resolution Neuroscience The Fate of Synaptic Vesicle Components upon Fusion Neurotransmitter release relies on the fusion of synaptic vesicles with the plasma membrane of synaptic boutons, which is followed by the recycling of vesicle components and formation of new vesicles. It is not yet clear whether upon fusion the vesicles persist as multimolecular patches in the plasma membrane, or whether they segregate into individual components. https://www.leica-microsystems.com//science-lab/the-fate-of-synaptic-vesicle-components-upon-fusion/ Fri, 01 Oct 2010 11:41:00 +0000 Dr. Felipe Opazo https://www.leica-microsystems.com/3833 Laser Microdissection Neuroscience The Mitochondrial Hypothesis of Ageing Why do we grow old? Research scientists have been looking for an answer to this question for many years – particularly against the background of the increase in neurodegenerative diseases among older people such as Morbus Parkinson. https://www.leica-microsystems.com//science-lab/the-mitochondrial-hypothesis-of-ageing/ Mon, 07 Sep 2009 16:11:00 +0000 Dr. Andreas Bender, Dipl. oec.-troph. Anja Schué https://www.leica-microsystems.com/8111 Neuroscience Live-Cell Imaging New Standard in Electrophysiology and Deep Tissue Imaging The function of nerve and muscle cells relies on ionic currents flowing through ion channels. These ion channels play a major role in cell physiology. One way to investigate ion channels is to use patch clamping. This method allows investigation of ion channels in detail and recording of the electric activity of different types of cells, mainly excitable cells like neurons, muscle fibres or beta cells of the pancreas. The patch clamping technique was developed by Erwin Neher and Bert Sakmann in the 1970s and 80s to study individual ion channels in living cells. In 1991 they received the Nobel Prize for Physiology and Medicine for their work. Today the patch clamping technique is one of the most important methods in the field of electrophysiology. https://www.leica-microsystems.com//science-lab/new-standard-in-electrophysiology-and-deep-tissue-imaging/ Tue, 17 Mar 2009 22:04:00 +0000 Dr. Irmtraud Steinmetz