Leica Science Lab - Tag : Plant Research https://www.leica-microsystems.com//science-lab/tag/tags/plant-research/show/Tag/ Article tagged with Plant Research en-US https://www.leica-microsystems.com/4421 Coherent Raman Scattering (CRS) Coherent Raman Scattering Microscopy Publication List CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their molecules. The two most important CRS techniques are Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). The biochemical image contrast of CRS is in many ways complementary to the molecular contrast obtained in fluorescence microscopy. A second crucial advantage of these methods is that they preserve the specimen/sample in a near pristine state. This reference list presents current and basic papers on CRS microscopy. https://www.leica-microsystems.com//science-lab/cars-publication-list/ Sun, 26 May 2019 22:00:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/3815 Laser Microdissection Neuroscience Forensics Laser Microdissection Publication List This monthly updated reference list demonstrates the major application fields for laser microdissection in life science research. https://www.leica-microsystems.com//science-lab/laser-microdissection-publication-list/ Sun, 28 Apr 2019 22:00:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/24510 White Confocal You Shall Not Pass! Time-gated Detection Takes Care of Endogenous Fluorescence in Plant Research. Yutaka Kodama is Associate Professor at the Biosciences Education Research Center, Utsunomiya University, Japan. He graduated from Saga University, Faculty of Agriculture, in 2004 and received his PhD from the Graduate School of Biological Science at the Nara Institute of Science and Technology in 2007. https://www.leica-microsystems.com//science-lab/you-shall-not-pass-time-gated-detection-takes-care-of-endogenous-fluorescence-in-plant-research/ Tue, 13 Nov 2018 23:00:00 +0000 https://www.leica-microsystems.com/20671 Confocal Microscopy MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1) Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little is known about the cellular mechanism regulating tapetal PCD. Here, we demonstrate that Rab7-mediated vacuolar transport of tapetum degradation-related cysteine proteases is crucial for tapetal PCD and pollen development in Arabidopsis (Arabidopsis thaliana), with the following evidence: (1) The monensin sensitivity1 (mon1) mutants, which are defective in Rab7 activation, showed impaired male fertility due to a combined defect in both tapetum and male gametophyte development. https://www.leica-microsystems.com//science-lab/monensin-sensitivity1-mon1calcium-caffeine-zinc-sensitivity1-ccz1/ Mon, 22 Oct 2018 22:00:00 +0000 https://www.leica-microsystems.com/19107 Fluorescence Microscopy Confocal Microscopy Testing the Münch Hypothesis of Long Distance Phloem Transport in Plants Long distance transport in plants occurs in sieve tubes of the phloem. The pressure flow hypothesis introduced by Ernst Münch in 1930 describes a mechanism of osmotically generated pressure differentials that are supposed to drive the movement of sugars and other solutes in the phloem, but this hypothesis has long faced major challenges. The key issue is whether the conductance of sieve tubes, including sieve plate pores, is sufficient to allow pressure flow. We show that with increasing distance between source and sink, sieve tube conductivity and turgor increases dramatically in Ipomoea nil. Our results provide strong support for the Münch hypothesis, while providing new tools for the investigation of one of the least understood plant tissues. https://www.leica-microsystems.com//science-lab/testing-the-muench-hypothesis-of-long-distance-phloem-transport-in-plants/ Tue, 14 Feb 2017 09:08:00 +0000 https://www.leica-microsystems.com/18960 Super-Resolution Image Restoration and Deconvolution Chloroplast-Mediated Regulation of CO2-Concentrating Mechanism by Ca2+-Binding Protein CAS in the Green Alga Chlamydomonas Reinhardtii Application example of HvYolution Super-Resolution - Aquatic photosynthetic organisms, including the green alga Chlamydomonas reinhardtii, induce a CO2-concentrating mechanism (CCM) to maintain photosynthetic activity in CO2-limiting conditions by sensing environmental CO2 and light availability. In this study, the introduction of an intact CAS gene into H82 cells restored photosynthetic affinity for inorganic carbon, and RNA-seq analyses revealed that CAS could function in maintaining the expression levels of nuclear-encoded CO2-limiting–inducible genes, including the HCO3– transporters high-light activated 3 (HLA3) and low-CO2–inducible gene A (LCIA). https://www.leica-microsystems.com//science-lab/chloroplast-mediated-regulation-of-co2-concentrating-mechanism-by-ca2-binding-protein-cas-in-the-green-alga-chlamydomonas-reinhardtii/ Mon, 06 Feb 2017 13:51:00 +0000 https://www.leica-microsystems.com/18098 Super-Resolution Actin-Dependent Vacuolar Occupancy of the Cell Determines Auxin-Induced Growth Repression The cytoskeleton is an early attribute of cellular life, and its main components are composed of conserved proteins. The actin cytoskeleton has a direct impact on the control of cell size in animal cells, but its mechanistic contribution to cellular growth in plants remains largely elusive. Here, we reveal a role of actin in regulating cell size in plants. The actin cytoskeleton shows proximity to vacuoles, and the phytohormone auxin not only controls the organization of actin filaments but also impacts vacuolar morphogenesis in an actin-dependent manner. https://www.leica-microsystems.com//science-lab/actin-dependent-vacuolar-occupancy-of-the-cell-determines-auxin-induced-growth-repression/ Fri, 03 Feb 2017 08:14:00 +0000 https://www.leica-microsystems.com/18207 EM Sample Preparation Picea abies (L.) KARST - Sample Preparation for TEM Application Note for Leica EM AMW - Plants (5-years old) were grown in pots filled with soil and kept in greenhouse conditions. Five weeks before harvesting the plants were transferred into growth chambers and cultivated at a temperature of 20°C during daytime and 12°C overnight. The relative humidity was set at 60% and the photoactive radiation was 500 μmol m-2 s-1 during daytime. Sample preparation for transmission electron microscopy (TEM) was performed in order to develop a standard protocol that would reduce sample preparation time for TEM-investigations. Therefore the overall and fine structure of leaf cells prepared with the Leica EM AMW were compared with leaf cells that were prepared with a conventional fixation protocol at room temperature. https://www.leica-microsystems.com//science-lab/picea-abies-l-karst-sample-preparation-for-tem/ Mon, 23 Jan 2017 19:24:00 +0000 Prof. Bernd Zechmann, Prof. Günther Zellnig https://www.leica-microsystems.com/18319 EM Sample Preparation Maple (Acer saccharum) Leaves - High Pressure Freezing and Freeze Substitution for TEM Application Note for Leica EM HPM100 - Leaves were immersed in hexadecene and placed under a gentle (0.3 bar) vacuum for 10 minutes to evacuate the internal air spaces. The leaves were then trimmed to fit the carriers and placed in the 200 μm side of a 6 mm Type A specimen carrier. Free space was filled with additional hexadecene after which a 6 mm Type B specimen carrier was placed on top with the flat side down. https://www.leica-microsystems.com//science-lab/maple-acer-saccharum-leaves-high-pressure-freezing-and-freeze-substitution-for-tem/ Mon, 16 Jan 2017 17:08:00 +0000 Dr. Kim Rensing https://www.leica-microsystems.com/18935 Fluorescence Microscopy Confocal Microscopy Multispectral Phloem-Mobile Probes: Properties and Applications Using Arabidopsis (Arabidopsis thaliana) seedlings, we identified a range of small fluorescent probes that entered the translocation stream and were unloaded at the root tip. These probes had absorbance/emission maxima ranging from 367/454 to 546/576 nm and represent a versatile toolbox for studying phloem transport. Of the probes that we tested, naturally occurring fluorescent coumarin glucosides (esculin and fraxin) were phloem loaded and transported in oocytes by the sucrose transporter, AtSUC2. Arabidopsis plants in which AtSUC2 was replaced with barley (Hordeum vulgare) sucrose transporter (HvSUT1), which does not transport esculin in oocytes, failed to load esculin into the phloem. https://www.leica-microsystems.com//science-lab/multispectral-phloem-mobile-probes-properties-and-applications/ Tue, 29 Nov 2016 16:44:00 +0000 https://www.leica-microsystems.com/18110 EM Sample Preparation Tobacco Leaf - Critical Point Drying Protocol for SEM Application Note for Leica EM CPD300 - Critical point drying of tobacco leafs with subsequent platinum coating and SEM analysis. https://www.leica-microsystems.com//science-lab/tobacco-leaf-critical-point-drying-protocol-for-sem/ Fri, 18 Nov 2016 16:21:00 +0000 Dr. Martin W. Goldberg, M.Sc. Christine Richardson https://www.leica-microsystems.com/18142 EM Sample Preparation Wall Cress Pod Protocol - Critical Point Drying of Arabidopsis thaliana for SEM Application Note for Leica EM CPD300 - Critical point drying of wall cress (Arabidopsis thaliana) pod with subsequent gold coating and SEM analysis. https://www.leica-microsystems.com//science-lab/wall-cress-pod-protocol-critical-point-drying-of-arabidopsis-thaliana-for-sem/ Tue, 25 Oct 2016 08:00:00 +0000 Dr. Chen LiYu https://www.leica-microsystems.com/17998 EM Sample Preparation Arabidopsis thaliana(L.) Accession Col Application Note for Leica EM AMW - Plants were grown in growth chambers under defined conditions. After stratification for 4 days at 4°C seeds were grown in pots with soil with 9/15 hours day/night photoperiod. Day and night temperatures were 22°C and 18°C, respectively, the relative humidity was 60% and the plants were kept at 100% relative soil water content. Light intensity varied between 110 and 140 μmol m-2 s-1. https://www.leica-microsystems.com//science-lab/arabidopsis-thalianal-accession-col/ Mon, 01 Aug 2016 13:03:00 +0000 Prof. Bernd Zechmann, Prof. Günther Zellnig https://www.leica-microsystems.com/17941 Laser Microdissection Workflows & Protocols: Plant Laser Microdissection During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection using the Leica LMD systems was demonstrated. One special focus was on plant dissection which needs a high laser power. https://www.leica-microsystems.com//science-lab/workflows-protocols-plant-laser-microdissection/ Mon, 06 Jun 2016 05:22:00 +0000 Dr. Falk Schlaudraff, Dr. Christoph Greb https://www.leica-microsystems.com/17444 Digital Microscopy Image gallery: Life Science Imaging with the Leica DVM6 Digital Microscope Digital microscopes can be a great help in life science applications such as the documentation in botany, entomology studies and crop science, or the digitization of museum collections. The image quality digital microscopes yield, is stunning – this image gallery gives you an idea. Enjoy! https://www.leica-microsystems.com//science-lab/galleries/image-gallery-digital-microscopy/image-gallery-life-science-imaging-with-the-leica-dvm6-digital-microscope/ Mon, 22 Feb 2016 15:42:00 +0000 PhD James DeRose, PhD Heinrich Bürgers https://www.leica-microsystems.com/14890 EM Sample Preparation Confocal Microscopy Influence of Tissue and Plant Species in the Trafficking of a Recombinant Protein in Plant Cells The development of recombinant DNA technology has allowed the use of plants for the production of biopharmaceuticals. In contrast to other production platforms, plants are unexpensive, easy to scale up and lack human pathogens. Moreover, because plants are eukaryotes they can process and modify complex human proteins. https://www.leica-microsystems.com//science-lab/influence-of-tissue-and-plant-species-in-the-trafficking-of-a-recombinant-protein-in-plant-cells/ Mon, 26 Jan 2015 17:57:00 +0000 PhD Elsa Arcalis, PhD, MSc Eva Stöger https://www.leica-microsystems.com/14863 EM Sample Preparation Recovery of Recombinant Antibody is Affected by Endogenous Protein Interaction in Maize Seeds provide a useful and versatile platform for the production of recombinant proteins and their numerous advantages have been often discussed. Among seeds, cereal crops offer additional advantages such as high yield and well-stablished agricultural infrastructure which allows easy up and down scaling in response to demand. https://www.leica-microsystems.com//science-lab/recovery-of-recombinant-antibody-is-affected-by-endogenous-protein-interaction-in-maize/ Mon, 15 Dec 2014 13:39:00 +0000 Dr. Jenny Peters https://www.leica-microsystems.com/14455 EM Sample Preparation Electron Tomography of Cryo-Immobilized Plant Tissue: A Novel Approach to Studying 3D Macromolecular Architecture of Mature Plant Cell Walls In Situ Cost-effective production of lignocellulosic biofuel requires efficient breakdown of cell walls present in plant biomass to retrieve the wall polysaccharides for fermentation. In-depth knowledge of plant cell wall composition is therefore essential for improving the fuel production process. The precise spatial three-dimensional (3D) organization of cellulose, hemicellulose, pectin and lignin within plant cell walls remains unclear to date since the microscopy techniques used so far have been limited to two-dimensional, topographic or low-resolution imaging, or required isolation or chemical extraction of the cell walls. https://www.leica-microsystems.com//science-lab/electron-tomography-of-cryo-immobilized-plant-tissue-a-novel-approach-to-studying-3d-macromolecular-architecture-of-mature-plant-cell-walls-in-situ/ Mon, 17 Nov 2014 17:47:00 +0000 https://www.leica-microsystems.com/13495 Laser Microdissection Preparation of Laser Microdissected Plant Cell and Tissue Material for Microchemical Analysis Secondary plant metabolites are involved in various plant physiological and biochemical processes, allowing plants to interact successfully with their biotic and abiotic environment. Distribution patterns of these compounds at the tissue and cellular level show how plant defense systems are organized to manage environmental challenges in a targeted and precise way. https://www.leica-microsystems.com//science-lab/preparation-of-laser-microdissected-plant-cell-and-tissue-material-for-microchemical-analysis/ Tue, 24 Jun 2014 09:08:00 +0000 PhD Dirk Hölscher, PhD Sara Agnolet, PhD Jingjing Fang, PhD Jonathan Gershenzon, PhD Oliver Kayser, PhD Sheng-Hong Li, PhD Bernd Schneider https://www.leica-microsystems.com/12428 Confocal Microscopy Fluorescence Microscopy Colonization of Potato Rhizosphere by GFP-Tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 Shown on Large Sections of Roots Using Enrichment Sample Preparation and Confocal Laser Scanning Microscopy The ability to colonize the host plants’ rhizospheres is a crucial feature to studyin the case of Plant Growth Promoting Rhizobacteria (PGPRs) with potential agricultural applications. In this work, we have created GFP-tagged derivatives of three candidate PGPRs: Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44. https://www.leica-microsystems.com//science-lab/colonization-of-potato-rhizosphere-by-gfp-tagged-bacillus-subtilis-mb732-pseudomonas-sp-p482-and-ochrobactrum-sp-a44-shown-on-large-sections-of-roots-using-enrichment-sample-preparation-and-confocal-laser-scanning-microscopy/ Fri, 02 May 2014 15:04:00 +0000 https://www.leica-microsystems.com/12989 Confocal Microscopy Genomic Survey, Gene Expression Analysis and Structural Modeling Suggest Diverse Roles of DNA Methyltransferases in Legumes DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases), namely Methyltransferase (MET), Chromomethylase (CMT) and Domains Rearranged Methyltransferase (DRM), which maintain methylation at CG, CHG and CHH sites. DNA MTases have not been studied in legumes so far. Here, we report the identification and analysis of putative DNA MTases in five legumes, including chickpea, soybean, pigeonpea, Medicago and Lotus. MTases in legumes could be classified in known MET, CMT, DRM and DNA nucleotide methyltransferases (DNMT2) subfamilies based on their domain organization. https://www.leica-microsystems.com//science-lab/genomic-survey-gene-expression-analysis-and-structural-modeling-suggest-diverse-roles-of-dna-methyltransferases-in-legumes/ Fri, 14 Mar 2014 11:29:00 +0000 https://www.leica-microsystems.com/7817 Laser Microdissection Quantitative One-Step Protocol to Detect Transcripts in Laser Microdissected Samples Arbuscular mycorrhizal (AM) fungi are an essential feature of the biology and ecology of most terrestrial plants and, as biofertilizers, AM fungi are an emerging issue in many projects focused on low-input agriculture practices. The identification of the events that lead to the formation of an AM, including the mechanisms involved in nutrient transfer, will be a challenging objective for a better exploitation of AMs in agricultural programs. https://www.leica-microsystems.com//science-lab/quantitative-one-step-protocol-to-detect-transcripts-in-laser-microdissected-samples/ Wed, 21 Nov 2012 23:00:00 +0000 PhD Valentina Fiorilli, Marco Giovannetti, PhD Raffaella Maria Balestrini https://www.leica-microsystems.com/5995 Stereo Microscopy Quality Assurance Stereo microscopes in the EU’s Plant Inspection Exotic fruits and sun-kissed vegetables – we have long been accustomed to a huge selection of culinary delicacies that are available fresh in stores on a daily basis. Sometimes, however, these goods flown in from far away carry along unwanted passengers: pests, fungi, or viruses, which cannot be seen with the naked eye. https://www.leica-microsystems.com//science-lab/stereo-microscopes-in-the-eus-plant-inspection/ Sun, 06 May 2012 22:00:00 +0000 Claudia Moch https://www.leica-microsystems.com/3835 Laser Microdissection Applications of Laser Microdissection Laser microdissection and laser micromanipulation are suitable for gaining a differentiated insight into the function of genes and proteins, and are used for a wide range of applications in neurobiology and immunology as well as in the developmental and cell biology of animal and plant organisms. https://www.leica-microsystems.com//science-lab/applications-of-laser-microdissection/ Sun, 26 Jun 2011 22:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/3862 Laser Microdissection Neuroscience Users Report on the Relevance of Laser Microdissection for Their Research Results Laser dissection is used in a large number of research fields, e.g. neurology, cancer research, plant analysis. Here, user report on the research results they have attained by using laser microdissection. https://www.leica-microsystems.com//science-lab/users-report-on-the-relevance-of-laser-microdissection-for-their-research-results/ Tue, 12 Apr 2011 22:00:00 +0000 Kerstin Pingel