Leica Science Lab - Tag : Protein Trafficking https://www.leica-microsystems.com//science-lab/tag/tags/protein-trafficking/show/Tag/ Article tagged with Protein Trafficking en-US https://www.leica-microsystems.com/20208 Quantitative Fluorescence FLIM FRET and Biosensors: Versatile Tools for Biomedical Research Fluorescence Lifetime Imaging (FLIM) in combination with Förster Resonance Energy Transfer (FRET) has proven to be very beneficial for investigations in biomedical research for a wide range of structural elements and dynamic changes in cells. FRET allows to monitor molecular interactions, as the FRET signal depends strongly on the distance of the two FRET partners. This allows to investigate interaction of molecules, like ligand-receptor pairs, protein-protein interactions or interactions of effectors with DNA. https://www.leica-microsystems.com//science-lab/flim-fret-and-biosensors-versatile-tools-for-biomedical-research/ Sun, 10 Jun 2018 22:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/14890 EM Sample Preparation Confocal Microscopy Influence of Tissue and Plant Species in the Trafficking of a Recombinant Protein in Plant Cells The development of recombinant DNA technology has allowed the use of plants for the production of biopharmaceuticals. In contrast to other production platforms, plants are unexpensive, easy to scale up and lack human pathogens. Moreover, because plants are eukaryotes they can process and modify complex human proteins. https://www.leica-microsystems.com//science-lab/influence-of-tissue-and-plant-species-in-the-trafficking-of-a-recombinant-protein-in-plant-cells/ Mon, 26 Jan 2015 17:57:00 +0000 PhD Elsa Arcalis, PhD, MSc Eva Stöger https://www.leica-microsystems.com/11772 EM Sample Preparation Protein Trafficking in Cereal Seeds Seeds accumulate proteins and starch which will be broken down and mobilized upon germination. Among seeds, cereals constitute an example of a highly specialized storage tissue that constitutes up to 80% of the total seed volume (Watson 1987). The seed endomembrane system is highly specialized and seed storage proteins travel through the endomembrane system en route to the protein bodies, which are either derived from the Endoplasmic reticulum (ER) or of vacuolar origin (Muntz 1998). https://www.leica-microsystems.com//science-lab/protein-trafficking-in-cereal-seeds/ Mon, 10 Feb 2014 17:31:00 +0000 PhD Elsa Arcalis, PhD, MSc Eva Stöger https://www.leica-microsystems.com/14848 Confocal Microscopy Apical Cargo Traverses Endosomal Compartments on the Passage to the Cell Surface Epithelial polarity is based on intracellular sorting machinery that maintains the asymmetric distribution of lipids and proteins to the cell surface. Dependent on their lipid raft affinity, newly synthesized apical polypeptides are segregated into distinct vesicle populations subsequent to the passage through the Golgi apparatus. https://www.leica-microsystems.com//science-lab/apical-cargo-traverses-endosomal-compartments-on-the-passage-to-the-cell-surface/ Mon, 15 Sep 2008 17:25:00 +0000