Leica Science Lab - Tag : Refractive Index https://www.leica-microsystems.com//science-lab/tag/tags/refractive-index/show/Tag/ Article tagged with Refractive Index en-US https://www.leica-microsystems.com/19673 Basics in Microscopy Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These include resolution, numerical aperture (NA), the working distance of objectives and the refractive index of the medium through which the image is collected by the front lens of an objective. In this article, we will briefly look at how using an immersion medium between the coverslip and the objective front lens helps to increase the NA and resolution. https://www.leica-microsystems.com//science-lab/immersion-objectives-using-oil-glycerol-or-water-to-overcome-some-of-the-limits-of-resolution/ Wed, 09 Aug 2017 12:07:00 +0000 PhD Martin Wilson https://www.leica-microsystems.com/19605 Basics in Microscopy Collecting Light: The Importance of Numerical Aperture in Microscopy Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA is a number without units and is related to the angles of light which are collected by a lens. In calculating NA (see below), the refractive index of a medium is also taken into account and by matching the refractive index of a slide or cell culture container with an immersion medium, then more of the detail of a specimen will be resolved. The way in which light behaves when travelling from one medium to another is also related to NA (and termed ‘refraction’). This article also covers a brief history of refraction and how this concept is a limiting factor in achieving high NA. https://www.leica-microsystems.com//science-lab/collecting-light-the-importance-of-numerical-aperture-in-microscopy/ Wed, 12 Jul 2017 07:46:00 +0000 PhD Martin Wilson https://www.leica-microsystems.com/18925 Super-Resolution Confocal Microscopy Image Restoration and Deconvolution Methods to Calibrate and Scale Axial Distances in Confocal Microscopy as a Function of Refractive Index Application example of HyVolution Super-Resolution - Accurate distance measurement in 3D confocal microscopy is important for quantitative analysis, volume visualization and image restoration. However, axial distances can be distorted by both the point spread function (PSF) and by a refractive-index mismatch between the sample and immersion liquid, which are difficult to separate. Additionally, accurate calibration of the axial distances in confocal microscopy remains cumbersome, although several high-end methods exist. In this paper we present two methods to calibrate axial distances in 3D confocal microscopy that are both accurate and easily implemented. https://www.leica-microsystems.com//science-lab/methods-to-calibrate-and-scale-axial-distances-in-confocal-microscopy-as-a-function-of-refractive-index/ Fri, 09 Dec 2016 08:29:00 +0000 https://www.leica-microsystems.com/16095 Stereo Microscopy How to Correct Aberration in Stereo Microscopy by Using the Right Objective Lenses For samples/specimens immersed in a liquid or embedded in a polymer, high quality microscopic observation can be hindered as a result of spherical aberration. An objective which can correct for refractive index mismatch allows images with greatly reduced spherical aberration and sharper focus to be obtained. https://www.leica-microsystems.com//science-lab/how-to-correct-aberration-in-stereo-microscopy-by-using-the-right-objective-lenses/ Fri, 21 Aug 2015 17:27:00 +0000 PhD James DeRose, PhD Heinrich Bürgers https://www.leica-microsystems.com/15458 Confocal Microscopy Fluorescence Microscopy Improving Axial Resolution in Confocal Microscopy with New High Refractive Index Mounting Media Resolution, high signal intensity and elevated signal to noise ratio (SNR) are key issues for biologists who aim at studying the localisation of biological structures at the cellular and subcellular levels using confocal microscopy. The resolution required to separate sub-cellular biological structures is often near to the resolving power of the microscope. https://www.leica-microsystems.com//science-lab/improving-axial-resolution-in-confocal-microscopy-with-new-high-refractive-index-mounting-media/ Thu, 11 Jun 2015 13:04:00 +0000