Leica Science Lab - Tag : RESOLFT https://www.leica-microsystems.com//science-lab/tag/tags/resolft/show/Tag/ Article tagged with RESOLFT en-US https://www.leica-microsystems.com/17611 Super-Resolution Multiphoton Microscopy Live-Cell Imaging 4Pi-RESOLFT Nanoscopy Here we apply the 4Pi scheme to RESOLFT nanoscopy using two-photon absorption for the on-switching of fluorescent proteins. We show that in this combination, the lobes are so low that low-light level, 3D nanoscale imaging of living cells becomes possible. Our method thus offers robust access to densely packed, axially extended cellular regions that have been notoriously difficult to super-resolve. Our approach also entails a fluorescence read-out scheme that translates molecular sensitivity to local off-switching rates into improved signal-to-noise ratio and resolution. https://www.leica-microsystems.com//science-lab/4pi-resolft-nanoscopy/ Fri, 26 Feb 2016 12:41:00 +0000 https://www.leica-microsystems.com/16796 Super-Resolution Video: Fluorescence is a State of Mind How to break a fundamental law of physics and win a Nobel Prize to boot. Stefan Hell explains super-resolved fluorescence microscopy for which he shared the 2014 Nobel Prize in chemistry. https://www.leica-microsystems.com//science-lab/video-fluorescence-is-a-state-of-mind/ Thu, 07 Jan 2016 20:45:00 +0000 Prof. Dr. Dr. h.c. Stefan Hell https://www.leica-microsystems.com/15700 Super-Resolution Live-Cell Imaging CRISPR-Cas9 CRISPR/Cas9-mediated Endogenous Protein Tagging for RESOLFT Super-Resolution Microscopy of Living Human Cells Overexpression is a notorious concern in conventional and especially in super-resolution fluorescence light microscopy studies because it may cause numerous artifacts including ectopic sub-cellular localizations, erroneous formation of protein complexes, and others. Nonetheless, current live cell super-resolution microscopy studies generally rely on the overexpression of a host protein fused to a fluorescent protein. https://www.leica-microsystems.com//science-lab/crisprcas9-mediated-endogenous-protein-tagging-for-resolft-super-resolution-microscopy-of-living-human-cells/ Wed, 22 Jul 2015 16:24:00 +0000 https://www.leica-microsystems.com/15059 Super-Resolution Finding, Defining and Breaking the Diffraction Barrier in Microscopy – A Historical Perspective Diffraction plays a crucial role in microscopy as it prevents the recording of arbitrarily sharp images with conventional light microscopes. Many names are connected with the notion of diffraction and the definition of resolution. An overview over the contributions of the different scientists to the recognition and definition of the diffraction barrier in the past centuries is given and the recent developments that led to breaking this barrier are portrayed. https://www.leica-microsystems.com//science-lab/finding-defining-and-breaking-the-diffraction-barrier-in-microscopy-a-historical-perspective/ Mon, 12 Jan 2015 15:42:00 +0000 https://www.leica-microsystems.com/13950 Super-Resolution Neuroscience STED Microscopy of Living Cells – New Frontiers in Membrane and Neurobiology Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review the current approaches to super-resolution optical microscopy and present the implementation of STED microscopy for novel insights into live cell mechanisms, with a focus on neurobiology and plasma membrane dynamics. https://www.leica-microsystems.com//science-lab/sted-microscopy-of-living-cells-new-frontiers-in-membrane-and-neurobiology/ Tue, 12 Aug 2014 12:11:00 +0000 Prof. Christian Eggeling https://www.leica-microsystems.com/13527 Super-Resolution Video Talk on Super-Resolution: Overview and Stimulated Emission Depletion (STED) Microscopy Historically, light microscopy has been limited in its ability to resolve closely spaced objects, with the best microscopes only able to resolve objects separated by 200 nm or more. This limit is known as the diffraction limit. In the last twenty years, a number of techniques have been developed that allow resolution beyond the diffraction limit. https://www.leica-microsystems.com//science-lab/video-talk-on-super-resolution-overview-and-stimulated-emission-depletion-sted-microscopy/ Thu, 05 Jun 2014 17:05:00 +0000 Prof. Dr. Dr. h.c. Stefan Hell https://www.leica-microsystems.com/10826 Super-Resolution STED Microscopy: A New Chapter in Light Imaging When Stefan Hell invented a technique that pushed beyond the diffraction limit of light for the first time, a new field of nanoscale imaging was born. Now laboratories around the world are using STED microscopy to smash through more boundaries in fundamental science. https://www.leica-microsystems.com//science-lab/sted-microscopy-a-new-chapter-in-light-imaging/ Mon, 16 Sep 2013 10:28:00 +0000 https://www.leica-microsystems.com/10135 Super-Resolution Widefield Microscopy Image Restoration and Deconvolution Abstracts of the 3rd European Super-Resolution User-Club Meeting The 3rd meeting of the Leica Super-Resolution User Club was held from June 17th to 19th, 2013 in collaboration with Alberto Diaspro and the Italian Institute of Technology (IIT) in Genoa. Confocal and widefield super-resolution users from ten European countries took three days’ out to deepen their knowledge on super-resolution techniques and applications and make use of an opportunity for full exchange of experiences. https://www.leica-microsystems.com//science-lab/abstracts-of-the-3rd-european-super-resolution-user-club-meeting/ Mon, 08 Jul 2013 10:27:00 +0000 Prof. Alberto Diaspro, Ph.D. Illaria Testa, Prof. Silvio Rizzoli, Ph.D. Giuseppe Vicidomini, Prof. Ralf Jacob, Ph.D. Eric Hosy, Prof. Colin Sheppard, Zeno Lavagnino https://www.leica-microsystems.com/7735 Super-Resolution Widefield Microscopy Image Restoration and Deconvolution Abstracts of the 2nd European Super-Resolution User-Club Meeting The 2nd meeting of the Leica Super-resolution User club was held from September 25 to 27, 2012 in collaboration with the Science for Life Laboratory at the Karolinska Institute, Stockholm, Sweden. With a mixture of engaging talks by key experts in the field of super-resolution microscopy and stimulating discussion sessions, the meeting proved as popular as last year’s event, attracting a wide range of scientists interested in both confocal and widefield super-resolution and sample preparation techniques. https://www.leica-microsystems.com//science-lab/abstracts-of-the-2nd-european-super-resolution-user-club-meeting/ Thu, 01 Nov 2012 23:00:00 +0000 Prof. Hjalmar Brismar, Prof. Dr. Dr. h.c. Stefan Hell, Prof. Christian Eggeling, Dr. Anna Szymborska, Prof. Alberto Diaspro, Ph.D. Eric Hosy, Ph.D. Giuseppe Vicidomini, Hans van der Voort