Leica Science Lab - Tag : RNA https://www.leica-microsystems.com//science-lab/tag/tags/rna-1/show/Tag/ Article tagged with RNA en-US https://www.leica-microsystems.com/19239 CRISPR-Cas9 Video: Genome Engineering with CRISPR-Cas9 Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studies that were previously unachievable. https://www.leica-microsystems.com//science-lab/video-genome-engineering-with-crispr-cas9/ Fri, 09 Jun 2017 06:50:00 +0000 https://www.leica-microsystems.com/19530 CRISPR-Cas9 Video: Genome Editing with CRISPR-Cas9 Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studies that were previously unachievable. https://www.leica-microsystems.com//science-lab/video-genome-editing-with-crispr-cas9/ Thu, 08 Jun 2017 06:50:00 +0000 https://www.leica-microsystems.com/19232 Fluorescence Microscopy CRISPR-Cas9 Five Big Mysteries about CRISPR’s Origins Francisco Mojica was not the first to see CRISPR, but he was probably the first to be smitten by it. He remembers the day in 1992 when he got his first glimpse of the microbial immune system that would launch a biotechnology revolution. He was reviewing genome-sequence data from the salt-loving microbe Haloferax mediterranei and noticed 14 unusual DNA sequences, each 30 bases long. https://www.leica-microsystems.com//science-lab/five-big-mysteries-about-crisprs-origins/ Mon, 20 Feb 2017 08:49:00 +0000 https://www.leica-microsystems.com/19182 EM Sample Preparation Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being internalized, possess the ability to modulate vital processes in recipient cells. In this study, with SOLiD sequencing technology, we performed identification, classification, and quantification of RNAs from blood fractions: cells, plasma, plasma vesicles pelleted at 16,000 https://www.leica-microsystems.com//science-lab/variety-of-rnas-in-peripheral-blood-cells-plasma-and-plasma-fractions/ Mon, 30 Jan 2017 10:22:00 +0000 https://www.leica-microsystems.com/18558 Laser Microdissection MicroRNA Expression Profiling to Identify and Validate Reference Genes for the Relative Quantification of microRNA in Rectal Cancer Application example for laser microdissection (LMD) - MicroRNAs (miRNAs) play important roles in regulating biological processes at the post-transcriptional level. Deregulation of miRNAs has been observed in cancer, and miRNAs are being investigated as potential biomarkers regarding diagnosis, prognosis and prediction in cancer management. Real-time quantitative polymerase chain reaction (RT-qPCR) is commonly used, when measuring miRNA expression. Appropriate normalisation of RT-qPCR data is important to ensure reliable results. The aim of the present study was to identify stably expressed miRNAs applicable as normaliser candidates in future studies of miRNA expression in rectal cancer. https://www.leica-microsystems.com//science-lab/microrna-expression-profiling-to-identify-and-validate-reference-genes-for-the-relative-quantification-of-microrna-in-rectal-cancer/ Wed, 07 Dec 2016 14:44:00 +0000 https://www.leica-microsystems.com/18885 Fluorescence Microscopy CRISPR-Cas9 Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target sequence via Watson-Crick base pairing. It serves as a marker for the foreign nucleic acid. The second component is the Cas9 protein. It binds to the marked sequence and cuts it due to its nuclease activity. Because the base pairing RNA can be synthesized easily and then used to determine a target region, researchers have utilized this system in the laboratory for genome editing. https://www.leica-microsystems.com//science-lab/gene-editing-with-crisprcas9-breakthrough-in-genome-engineering/ Thu, 17 Nov 2016 18:54:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/18952 Super-Resolution Live-Cell Imaging Image Restoration and Deconvolution Discovery of Novel Peptides Targeting Pro-Atherogenic Endothelium in Disturbed Flow Regions -Targeted siRNA Delivery to Pro-Atherogenic Endothelium in vivo Application example of HyVolution Super-Resolution - Atherosclerosis occurs preferentially in arterial regions exposed to disturbed blood flow. Targeting these pro-atherogenic regions is a potential anti-atherogenic therapeutic approach, but it has been extremely challenging. Here, using in vivo phage display approach and the partial carotid ligation model of flow-induced atherosclerosis in mouse, we identified novel peptides that specifically bind to endothelial cells (ECs) exposed to disturbed flow condition in pro-atherogenic regions. https://www.leica-microsystems.com//science-lab/discovery-of-novel-peptides-targeting-pro-atherogenic-endothelium-in-disturbed-flow-regions-targeted-sirna-delivery-to-pro-atherogenic-endothelium-in-vivo/ Thu, 10 Nov 2016 15:28:00 +0000 https://www.leica-microsystems.com/18691 Stereo Microscopy Confocal Microscopy Fluorescence Microscopy Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans For scientists, technicians, and teachers working with the worm C. elegans in the research lab or classroom, this report is intended to give useful information to help improve their daly work. The aim is to make the work steps of worm picking, transgenesis, RNA interference, screening, and functional imaging efficient. It also details the various possibilities for equipping a research worm lab or biology classroom/teaching lab explaining worm methods. https://www.leica-microsystems.com//science-lab/work-efficiently-in-developmental-biology-with-stereo-and-confocal-microscopy-c-elegans/ Mon, 19 Sep 2016 06:09:00 +0000 PhD James DeRose, PhD Heinrich Bürgers, PhD Martin Gamerdinger https://www.leica-microsystems.com/18623 Fluorescence Microscopy CRISPR-Cas9 Video Talk: CRISPR-Cas – From a Bacterial Adaptive Immune System to a Genome Engineering Tool The CRISPR-Cas system was originally discovered as an adaptive immune system of bacteria and archaea to protect against viral attack. During this talk, given at Leica Microsystems in Wetzlar, Dr. Lennart Randau, MPI Marburg, presents this simple and fascinating system in detail. Furthermore, he introduces the adaption of the CRISPR-Cas system into a potent molecular biology tool, which is used heavily for genome editing. In addition to its influence on molecular biology, meanwhile the Cas9 nuclease also stimulates microscopy techniques e.g. to fluorescently label genomic loci in living cells. https://www.leica-microsystems.com//science-lab/video-talk-crispr-cas-from-a-bacterial-adaptive-immune-system-to-a-genome-engineering-tool/ Tue, 12 Jul 2016 04:13:00 +0000 Dr. Lennart Randau https://www.leica-microsystems.com/18108 White Confocal Fluorescence Microscopy The Oncogenic Triangle of HMGA2, LIN28B and IGF2BP1 Antagonizes Tumor-Suppressive Actions of the let-7 Family The tumor-suppressive let-7 microRNA family targets various oncogene-encoding mRNAs. We identify the let-7 targets HMGA2, LIN28B and IGF2BP1 to form a let-7 antagonizing self-promoting oncogenic triangle. Surprisingly, 3′-end processing of IGF2BP1 mRNAs is unaltered in aggressive cancers and tumor-derived cells although IGF2BP1 synthesis was proposed to escape let-7 attack by APA-dependent (alternative polyadenylation) 3′ UTR shortening. https://www.leica-microsystems.com//science-lab/the-oncogenic-triangle-of-hmga2-lin28b-and-igf2bp1-antagonizes-tumor-suppressive-actions-of-the-let-7-family/ Fri, 17 Jun 2016 13:49:00 +0000 https://www.leica-microsystems.com/17977 Confocal Microscopy Live-Cell Imaging The Bimodally Expressed MicroRNA miR‐142 Gates Exit from Pluripotency A stem cell's decision to self‐renew or differentiate is thought to critically depend on signaling cues provided by its environment. It is unclear whether stem cells have the intrinsic capacity to control their responsiveness to environmental signals that can be fluctuating and noisy. Using a novel single‐cell microRNA activity reporter, we show that miR‐142 is bimodally expressed in embryonic stem cells, creating two states indistinguishable by pluripotency markers. https://www.leica-microsystems.com//science-lab/the-bimodally-expressed-microrna-mir-142-gates-exit-from-pluripotency/ Fri, 13 May 2016 13:47:00 +0000 https://www.leica-microsystems.com/16521 Super-Resolution Live-Cell Imaging Fluorescence Microscopy Probes that FIT RNA We have been developing new tools based on fluorogenic forced intercalation (FIT) probes for RNA detection quantification and interference in biological samples. Upon duplex formation with target nucleic acids, the base surrogates TO dye increases its quantum yield and brightness substantially (>10 fold). https://www.leica-microsystems.com//science-lab/probes-that-fit-rna/ Tue, 22 Sep 2015 17:25:00 +0000 PhD Imre Gaspar