Leica Science Lab - Tag : Self Pressurized Freezing https://www.leica-microsystems.com//science-lab/tag/tags/self-pressurized-freezing/show/Tag/ Article tagged with Self Pressurized Freezing en-US https://www.leica-microsystems.com/11782 EM Sample Preparation Freeze Substitution of Trypanosoma brucei Chemical fixation of biological specimens for ultrastructural investigation is a relatively slow and selective process, and therefore a common source of artifacts. Freezing, on the other hand, is an excellent method to physically fix biological specimens in their entirety and without delay; the formation of ice crystals large enough to displace cellular material and destroy structures, would be, however, a major issue. https://www.leica-microsystems.com//science-lab/freeze-substitution-of-trypanosoma-brucei/ Tue, 18 Mar 2014 17:25:00 +0000 Harald Kotisch, Dr. Katy Schmidt, Dr. Jan Leunissen, Dr. Guenter Resch