Leica Science Lab - Tag : Signal Protein https://www.leica-microsystems.com//science-lab/tag/tags/signal-protein/show/Tag/ Article tagged with Signal Protein en-US https://www.leica-microsystems.com/18960 Super-Resolution Image Restoration and Deconvolution Chloroplast-Mediated Regulation of CO2-Concentrating Mechanism by Ca2+-Binding Protein CAS in the Green Alga Chlamydomonas Reinhardtii Application example of HvYolution Super-Resolution - Aquatic photosynthetic organisms, including the green alga Chlamydomonas reinhardtii, induce a CO2-concentrating mechanism (CCM) to maintain photosynthetic activity in CO2-limiting conditions by sensing environmental CO2 and light availability. In this study, the introduction of an intact CAS gene into H82 cells restored photosynthetic affinity for inorganic carbon, and RNA-seq analyses revealed that CAS could function in maintaining the expression levels of nuclear-encoded CO2-limiting–inducible genes, including the HCO3– transporters high-light activated 3 (HLA3) and low-CO2–inducible gene A (LCIA). https://www.leica-microsystems.com//science-lab/chloroplast-mediated-regulation-of-co2-concentrating-mechanism-by-ca2-binding-protein-cas-in-the-green-alga-chlamydomonas-reinhardtii/ Mon, 06 Feb 2017 13:51:00 +0000 https://www.leica-microsystems.com/13942 Fluorescence Microscopy Quantitative Fluorescence Video Talk by Roger Tsien: Fluorescent Protein Indicators In this talk, Roger Tsien discusses how fluorescent proteins have been turned into indicators for a wide variety of biological molecules, including pH, ions, redox potential, and signaling molecules like phosphoinositides. The talk also covers reporters used to measure the activity of enzymes like kinases, phosphatases, and proteases. It covers both single proteins whose intensity or wavelength change, as well as reporters using Förster resonance energy transfer (FRET). https://www.leica-microsystems.com//science-lab/video-talk-by-roger-tsien-fluorescent-protein-indicators/ Tue, 16 Aug 2016 10:29:00 +0000 PhD Roger Y. Tsien https://www.leica-microsystems.com/9287 EM Sample Preparation Targeting of Peroxisomal Matrix Proteins in the Diatom Phaeodactylum Tricornutum P. tricornutum cells expressing different types of GFP fusion proteins were harvested via centrifugation at 1,500xg and cryoimmobilized by high-pressure freezing on gold carriers. https://www.leica-microsystems.com//science-lab/targeting-of-peroxisomal-matrix-proteins-in-the-diatom-phaeodactylum-tricornutum/ Sun, 17 Mar 2013 23:00:00 +0000 Dr. Andreas Klingl https://www.leica-microsystems.com/7275 Laser Microdissection Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome" The physiological process of aging is an elementary part of life. The processes responsible for the aging process and the effect they have is one of the key focuses of biomedical research. Dr. Yvonne Begus-Nahrmann researches molecular mechanisms in stem cells and their role in aging and tumor development at the Göttingen University Medical Center. Signal proteins such as p53 and p21 appear to play an important role in this context. To investigate these proteins in specific cell types, the cells are isolated from organ tissue with the aid of laser microdissection. https://www.leica-microsystems.com//science-lab/stem-cells-in-the-aging-process-the-signal-protein-p53-as-guardian-of-the-genome/ Thu, 08 Nov 2012 23:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille, Dr. Yvonne Begus-Nahrmann