Leica Science Lab - Tag : Signal-to-Noise https://www.leica-microsystems.com//science-lab/tag/tags/signal-to-noise/show/Tag/ Article tagged with Signal-to-Noise en-US https://www.leica-microsystems.com/24935 Fluorescence Microscopy Live-Cell Imaging Widefield Microscopy Image Gallery: THUNDER Imager To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve this using Computational Clearing our new opto-digital technology. The result is high-speed, high-quality imaging of a large diversity of three-dimensional samples, including model organisms, tissue sections, and 3D cell cultures. Take a look at these images to see how THUNDER Imagers are already helping researchers to reveal the finest structural details even deep within a sample. Once you have seen the haze-free image quality achieved with THUNDER Imagers, find out more on our product page. Learn how THUNDER Imagers combine Computational Clearing with the speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes so you can decode 3D biology in real-time. https://www.leica-microsystems.com//science-lab/galleries/image-gallery-thunder-imager/ Wed, 17 Apr 2019 22:00:00 +0000 https://www.leica-microsystems.com/24637 Widefield Microscopy Fluorescence Microscopy Live-Cell Imaging Stereo Microscopy THUNDER Technology Note So far, widefield microscopy was not suitable to image larger volumes, since the contrast of the recorded fluorescence image is reduced by the background (BG) mainly originating from out of focus regions of the recorded volume. BG reduces the effective dynamic range, the maximal possible signal to noise ratio (SNR), of the imaging system. https://www.leica-microsystems.com//science-lab/thunder-technology-note/ Tue, 05 Mar 2019 23:00:00 +0000 Dr. Jan Schumacher, Louise Bertrand https://www.leica-microsystems.com/20511 Image Restoration and Deconvolution Super-Resolution How to extract Image Information by Adaptive Deconvolution Confocal Laser Scannning Microscopy (CLSM) is the standard for true 3D resolved fluorescence imaging. Fast optical sectioning using flexible scanning strategies in combination with simultaneous multi-colour, high sensitivity and low noise signal detection provides maximum resolution in the spatial and temporal domain. In combination with modern approaches to image information extraction this helps the researcher to mine as much information as possible from the images acquired. Image information extraction refers to intelligent procedures for image enhancement using a priori knowledge from the imaging system. From simple glare control and optical development to intelligent and ingenious model extraction, there are many ways to see more than just the image. https://www.leica-microsystems.com//science-lab/how-to-extract-image-information-by-adaptive-deconvolution/ Tue, 18 Sep 2018 22:00:00 +0000 Dr. Jürgen Reymann https://www.leica-microsystems.com/17472 Camera Technology Basics in Microscopy Widefield Microscopy Image Restoration and Deconvolution Definitions of Basic Technical Terms for Digital Microscope Cameras and Image Analysis Most microscopes today are operated with a camera. The characteristics of the camera often decide whether the acquired image will reveal what a researcher wants to see. But when diving into camera terminology, the technical terms can be overwhelming. We have compiled the most important terms with a concise explanation to provide orientation. https://www.leica-microsystems.com//science-lab/definitions-of-basic-technical-terms-for-digital-microscope-cameras-and-image-analysis/ Tue, 19 Apr 2016 11:39:00 +0000 Claudia Müller, Dr. Christoph Greb, Dr. Karin Schwab https://www.leica-microsystems.com/17931 Camera Technology Live-Cell Imaging What Makes sCMOS Microscope Cameras so Popular? sCMOS cameras are more sensitive and are capable of much higher acquisition speed than cameras with other sensor types. Even though CCD cameras are widely used in live cell imaging and time-lapse recordings, researchers are often concerned that their camera does not detect faint signals. In this interview, Dr. Karin Schwab, Product Manager at Leica Microsystems, talks about the characteristics of sCMOS cameras and how researchers benefit from the latest camera sensor technology. https://www.leica-microsystems.com//science-lab/what-makes-scmos-microscope-cameras-so-popular/ Mon, 11 Apr 2016 10:22:00 +0000 Claudia Müller, Dr. Karin Schwab https://www.leica-microsystems.com/17468 Camera Technology Basics in Microscopy Widefield Microscopy Introduction to Digital Camera Technology A significant majority of modern optical microscopy techniques require the use of a digital camera. By working with digital devices researchers can observe specimens on a screen in real time or acquire and store images and quantifiable data. Here we introduce the basic principles behind digital camera technologies commonly encountered in scientific imaging. https://www.leica-microsystems.com//science-lab/introduction-to-digital-camera-technology/ Tue, 02 Feb 2016 16:40:00 +0000 Dr. Christoph Greb, Dr. Peter Laskey, Dr. Karin Schwab https://www.leica-microsystems.com/15928 Super-Resolution Gated STED Microscopy with Time-gated Single-photon Avalanche Diode The maximization of the useful (within the time gate) photon flux is then an important aspect to obtain super-resolved STED images. Here we show that by using a fast-gated single-photon avalanche diode (SPAD), i.e. a detector able to rapidly (hundreds picoseconds) switch-on and -off can improve significantly the signal-to-noise ratio (SNR) of the gated STED image. In addition to an enhancement of the image SNR, the use of the fast-gated SPAD reduces also the system complexity. We demonstrate these abilities both on calibration and biological sample. https://www.leica-microsystems.com//science-lab/gated-sted-microscopy-with-time-gated-single-photon-avalanche-diode/ Tue, 25 Aug 2015 17:24:00 +0000 https://www.leica-microsystems.com/13946 TIRF Microscopy Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique is used for, explains the principles of the evanescent wave, gives many examples of different microscope configurations used in TIRF, and shows how polarized light TIRF can be used to image membrane orientation. https://www.leica-microsystems.com//science-lab/video-talk-by-daniel-axelrod-total-internal-reflection-fluorescence-tirf-microscopy/ Mon, 09 Mar 2015 08:45:00 +0000 https://www.leica-microsystems.com/14980 Super-Resolution Image Restoration and Deconvolution Multi-Images Deconvolution Improves Signal-to-Noise Ratio on Gated Stimulated Emission Depletion Microscopy Time-gated detection, namely, only collecting the fluorescence photons after a time-delay from the excitation events, reduces complexity, cost, and illumination intensity of a stimulated emission depletion (STED) microscope. In the gated continuous-wave- (CW-) STED implementation, the spatial resolution improves with increased time-delay, but the signal-to-noise ratio (SNR) reduces. https://www.leica-microsystems.com//science-lab/multi-images-deconvolution-improves-signal-to-noise-ratio-on-gated-stimulated-emission-depletion-microscopy/ Fri, 23 Jan 2015 12:22:00 +0000 https://www.leica-microsystems.com/9410 Stereo Microscopy Fluorescence Microscopy Stereo microscopes with TripleBeam Technology Especially in fluorescence microscopy, excitation light is friend and foe in one. On the one hand, energy-rich excitation via a specific light wavelength of the fluorochrome resulting in a bright positive fluorochrome signal is highly welcome. On the other hand, "noise" caused by reflections of excitation light passing through the surfaces of optical elements needs to be extremely slight to generate a perfect black background. This relation is described as "signal-to-noise ratio", which is highly relevant for differentiating optically between fluorescence positive and negative cells. https://www.leica-microsystems.com//science-lab/stereo-microscopes-with-triplebeam-technology/ Tue, 02 Apr 2013 12:35:00 +0000 Björn Fuchs https://www.leica-microsystems.com/6677 Confocal Microscopy Sensors for True Confocal Scanning In this article, advantages and disadvantages of different types of sensors for single point true confocal scanning devices are discussed. Traditionally, photomultiplier tubes have been employed in such systems. For some cases, avalanche photodiodes have proven to fit best. A new development uniting vacuum and silicon technology has led to chimeric sensors, called hybrid detectors (HyD). They benefit from both technologies. https://www.leica-microsystems.com//science-lab/sensors-for-true-confocal-scanning/ Wed, 22 Aug 2012 22:00:00 +0000 Dr. Rolf T. Borlinghaus, Holger Birk, Frank Schreiber https://www.leica-microsystems.com/5945 TIRF Microscopy Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the evanescent wave, which arises from the glass/water or glass/specimen interface, is that its propagation in z-direction gradually degrades, limiting its penetration depth into the specimen to some hundred nanometers. https://www.leica-microsystems.com//science-lab/controlling-the-tirf-penetration-depth-is-mandatory-for-reproducible-results/ Tue, 24 Apr 2012 22:00:00 +0000 Dr. Thomas Veitinger https://www.leica-microsystems.com/5403 TIRF Microscopy Live-Cell Imaging Widefield Microscopy Total Internal Reflection Fluorescence (TIRF) Microscopy Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy delivers images with an outstandingly high axial resolution below 100 nm. This allows the observation of membrane-associated processes. https://www.leica-microsystems.com//science-lab/total-internal-reflection-fluorescence-tirf-microscopy/ Sun, 11 Mar 2012 23:00:00 +0000 Wymke Ockenga https://www.leica-microsystems.com/5405 Live-Cell Imaging TIRF Microscopy Widefield Microscopy Applications of TIRF Microscopy in Life Science Research The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or lasers, the evanescent field only penetrates the specimen by about 100 nm starting from the coverslip/medium interface. https://www.leica-microsystems.com//science-lab/applications-of-tirf-microscopy-in-life-science-research/ Sun, 11 Mar 2012 23:00:00 +0000 Wymke Ockenga, Dr. Thomas Veitinger https://www.leica-microsystems.com/10321 Quantitative Fluorescence A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range FRET-based sensors for cyclic Adenosine Mono Phosphate (cAMP) have revolutionized the way in which this important intracellular messenger is studied. The currently prevailing sensors consist of the cAMP-binding protein Epac1, sandwiched between suitable donor- and acceptor fluorescent proteins (FPs). https://www.leica-microsystems.com//science-lab/a-mturquoise-based-camp-sensor-for-both-flim-and-ratiometric-read-out-has-improved-dynamic-range/ Fri, 29 Apr 2011 14:47:00 +0000