Leica Science Lab - Tag : Single Cell Analysis https://www.leica-microsystems.com//science-lab/tag/tags/single-cell-analysis/show/Tag/ Article tagged with Single Cell Analysis en-US https://www.leica-microsystems.com/20495 Laser Microdissection Nanodroplet Processing Platform for Deep and Quantitative Proteome Profiling of 10–100 Mammalian Cells Nanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches require samples comprising a minimum of thousands of cells to provide in-depth profiling. Here, we report the development of a nanoPOTS (nanodroplet processing in one pot for trace samples) platform for small cell population proteomics analysis. NanoPOTS enhances the efficiency and recovery of sample processing by downscaling processing volumes to <200 nL to minimize surface losses. https://www.leica-microsystems.com//science-lab/nanodroplet-processing-platform-for-deep-and-quantitative-proteome-profiling-of-10-100-mammalian-cells/ Wed, 12 Sep 2018 22:00:00 +0000 https://www.leica-microsystems.com/19390 Laser Microdissection Correlation of Phenotype and Genotype – microscopic samples and their biomolecular backgrounds Seeing is believing, this is what makes microscopes, imaging systems, or box-type imagers so important for researchers in many different fields like pathology research, cancer research, neuroscience, and developmental research. Correlation of phenotype (what you can see) and genotype (genetic background) is highly desired but often biased by whole tissue approaches as no real solution is available to reliably separate the single cells of interest and surrounding tissue. Thus, results of DNA mutation analysis (sequencing), gene expression profiling (quantitative real-time PCR, qPCR, microarray, digital PCR), next generation sequencing (NGS) or mass spectrometry (MS) approaches can give mixed results as no pure starting material for the biomolecular methods of choice is available. https://www.leica-microsystems.com//science-lab/correlation-of-phenotype-and-genotype-microscopic-samples-and-their-biomolecular-backgrounds/ Thu, 20 Apr 2017 07:08:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/18860 Webinar: Laser Microdissection in Cancer Research – Mutation Analysis Workflow with Pure Cancer Material Cancer can affect various organs and is caused by mutations of the DNA. A prerequisite, to explore and understand underlying gene-mutations involved in the development of a definite type of cancer, is the extraction of pure sample material, which is challenging. In this webinar, we will present how to extract 100% pure cancer tissue for DNA analysis with laser microdissection (LMD). Using tissue samples from human kidney cancer patients as an example, this webinar will provide an overview of the practical considerations when preparing a workflow to obtain highly pure material with the LMD microscope for further molecular analysis. https://www.leica-microsystems.com//science-lab/webinar-laser-microdissection-in-cancer-research-mutation-analysis-workflow-with-pure-cancer-material/ Tue, 04 Oct 2016 08:57:00 +0000 PhD Tamara Straube, Prof. Ralf Jacob https://www.leica-microsystems.com/18742 Basics in Microscopy Widefield Microscopy Image Gallery: Application Options with Leica DM2500 LED Microscope The number of applications in microscopy can be as manifold as life itself. Different specimens demand different contrast methods to get a decent result. Unstained cells normally do not show a very high contrast. Researchers approach this problem by either staining the specimen with dyes respectively fluorescent molecules; or adding contrast by manipulating the light passing through. https://www.leica-microsystems.com//science-lab/galleries/image-gallery-application-options-with-leica-dm2500-led-microscope/ Tue, 23 Aug 2016 11:45:00 +0000 Dr. Christoph Greb, PhD Tamara Straube https://www.leica-microsystems.com/17939 Laser Microdissection Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a perfect tool for this purpose and how this was taught in the course of a workshop held in Brazil. With the Leica LMD system pure tumor material can be selected and dissected for downstream analysis to ensure 100% pure starting material without any risk of cross contamination with healthy cells. https://www.leica-microsystems.com//science-lab/workflows-protocols-laser-microdissection-for-pathology-and-cancer-research/ Wed, 04 May 2016 11:30:00 +0000 Dr. Falk Schlaudraff, Dr. Christoph Greb https://www.leica-microsystems.com/16814 Laser Microdissection Neuroscience Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as it allows to separate distinct brain layers or even to isolate individual neurons. https://www.leica-microsystems.com//science-lab/workflows-protocols-connecting-microscopy-and-molecular-biology-in-neuroscience/ Fri, 04 Dec 2015 16:01:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/16808 Laser Microdissection Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome. Another topic was the dissection of individual chromosomes from chromosome spreads. Leica specialists held a short training session with the LMD. After this, new LMD users were able to run the system and practiced how to dissect chromosomes and collect single chromosomes for downstream analysis. https://www.leica-microsystems.com//science-lab/workflows-protocols-how-to-isolate-individual-chromosomes-with-laser-microdissection/ Thu, 03 Dec 2015 20:06:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/16110 Laser Microdissection Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or sequencing techniques. Here we describe the successful combination of the Leica Microsystems LMD system and Qiagen kits for purification of nucleic acids even from little amounts. https://www.leica-microsystems.com//science-lab/workflows-protocols-how-to-use-a-leica-laser-microdissection-system-and-qiagen-kits-for-successful-rna-analysis/ Fri, 10 Jul 2015 14:26:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/13782 Laser Microdissection The Basic Principles of Laser Microdissection In contrast to other systems which use a fixed laserfocus for dissection, Leica Microsystems' LMD systems guide the laserfocus for dissection. This unique feature allows highly precise laser dissection independent of the stage accuracy. This tutorial describes the basic principles of the laser guidance and how the laser can be adjusted for perfect cutting results. https://www.leica-microsystems.com//science-lab/the-basic-principles-of-laser-microdissection/ Thu, 24 Jul 2014 13:48:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/2469 Laser Microdissection Neuroscience The Morbus Parkinson Puzzle A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this disease have been identified. In the case of the genetic familial form, for example, it has been possible to identify various genes that have a causal influence for M. Parkinson. https://www.leica-microsystems.com//science-lab/the-morbus-parkinson-puzzle/ Thu, 27 Mar 2014 11:11:00 +0000 Dr. Falk Schlaudraff, Dr. Olaf Spörkel https://www.leica-microsystems.com/9585 Laser Microdissection History of Laser Microdissection Modern laser microdissection technology has its roots in the early 20th century. It has been steadily advanced and modified over the years. The following article is a summary of the history of laser microdissection from its origins to today’s state of the art. https://www.leica-microsystems.com//science-lab/history-of-laser-microdissection/ Mon, 06 May 2013 19:38:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/6773 Laser Microdissection K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced Exploration Phasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies. This activation of DA midbrain neurons occurs via a synaptically triggered switch from low-frequency background spiking to transient high-frequency burst firing. We found that, in medial DA neurons of the substantia nigra (SN), activity of ATP-sensitive potassium (K-ATP) channels enabled NMDA-mediated bursting in vitro as well as spontaneous in vivo burst firing in anesthetized mice. https://www.leica-microsystems.com//science-lab/k-atp-channels-in-dopamine-substantia-nigra-neurons-control-bursting-and-novelty-induced-exploration/ Thu, 04 Oct 2012 22:00:00 +0000 Julia Schiemann, Dr. Falk Schlaudraff, Dr. Verena Klose, Markus Bingmer, Prof. Susumu Seino, Dr. Peter J. Magill, Ph.D. Kareem A. Zaghloul, Dr. Gaby Schneider, Prof. Birgit Liss, Prof. Jochen Röper https://www.leica-microsystems.com/3819 Laser Microdissection An Introduction to Laser Microdissection Laser microdissection is a highly selective process for preparing samples for DNA, RNA and protein analysis. It is a microscope-controlled manipulation technique for the precise separation of samples and tissue using a focused laser beam. https://www.leica-microsystems.com//science-lab/an-introduction-to-laser-microdissection/ Tue, 28 Jun 2011 15:39:57 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/3835 Laser Microdissection Applications of Laser Microdissection Laser microdissection and laser micromanipulation are suitable for gaining a differentiated insight into the function of genes and proteins, and are used for a wide range of applications in neurobiology and immunology as well as in the developmental and cell biology of animal and plant organisms. https://www.leica-microsystems.com//science-lab/applications-of-laser-microdissection/ Sun, 26 Jun 2011 22:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille