Leica Science Lab - Tag : SP8 Confocal technology https://www.leica-microsystems.com//science-lab/tag/tags/sp8-confocal-technology/show/Tag/ Article tagged with SP8 Confocal technology en-US https://www.leica-microsystems.com/24698 Confocal Microscopy What is a Resonant Scanner? A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition speed is required to track fast processes, especially in living samples. It has the additional benefit of providing a better fluorescence signal and reduced photobleaching. https://www.leica-microsystems.com//science-lab/what-is-a-resonant-scanner/ Sun, 10 Mar 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24696 Confocal Microscopy What is a Spectral Detector (SP Detector)? The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands. The bands are independent and continuously tunable within the full visible spectrum. The light in each band is detected by a light sensor: a photomultiplier tube (PMT) or a Hybrid Detector (HyD). https://www.leica-microsystems.com//science-lab/what-is-a-spectral-detector-sp-detector/ Sun, 10 Mar 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24713 Confocal Microscopy Resolved Field Number (RFN) The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot observe very large fields at once, the human eye can scan and integrate structural features over the full field. Furthermore, it is essential, that field size and resolution fit to the capabilities of the human eye. https://www.leica-microsystems.com//science-lab/resolved-field-number-rfn/ Wed, 06 Mar 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24711 Confocal Microscopy What is a Hybrid Detector (HyD)? A HyD is a sensor for detecting light (photons). It is a compound (hybrid) of two technologies: vacuum tubes, like those used in a photomultiplier tube (PMT), and semiconductor microelectronics, like an avalanche photodiode (APD). The Hybrid Detector combines essentially the advantages of both approaches. It is fast, shows very little noise, and offers a high dynamic range. For a comparison see references 1, 2, 3, and 4. The HyD is the best suited sensor for most confocal and multiphoton applications. https://www.leica-microsystems.com//science-lab/what-is-a-hybrid-detector-hyd/ Wed, 06 Mar 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24709 Confocal Microscopy What is a Field-of-View Scanner? A field-of-view scanner is an assembly of galvanometric scanning mirrors used in single-point confocal microscopes that offer the correct optical recording of large field sizes. The field-of-view scanner employs a three-mirror concept that offers superior homogeneity in illumination compared to classical two-mirror scanners, without compromising high scanning speed. The galvanometric scanners are controllable in speed and position, allowing zoom and pan functions as well as tuning the scan frequency. They also allow resting point illumination as is required for, e.g., FCS-measurements. https://www.leica-microsystems.com//science-lab/what-is-a-field-of-view-scanner/ Wed, 06 Mar 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24694 Confocal Microscopy What is a Tandem Scanner? A Tandem Scanner is an assembly of two different types of scanning together in one system for true confocal point scanning. The Tandem Scanner consists of a three-mirror scanning base with the x-scanner exchangeable with a motorized device. This combination allows scanning of large areas with high scan-resolution by a FOV-scanner and of very fast processes by a resonant scanner, both within the same instrument. https://www.leica-microsystems.com//science-lab/what-is-a-tandem-scanner/ Thu, 28 Feb 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus