Leica Science Lab - Tag : Stem Cells https://www.leica-microsystems.com//science-lab/tag/tags/stem-cells/show/Tag/ Article tagged with Stem Cells en-US https://www.leica-microsystems.com/26346 Multiphoton Microscopy Confocal Microscopy Multiphoton imaging bolsters findings on in vitro differentiation of pluripotent stem cell-derived podocyte The renal corpuscle of the kidney comprises a glomerular vasculature embraced by podocytes and supported by mesangial myofibroblasts. Their combined actions are essential for the formation of an acellular plasma filtrate that passes through the podocyte-generated slit diaphragm into the nephron. Mutations in podocyte-expressed genes lead to chronic disease. An enhanced understanding of podocyte development and function to create biologically relevant in vitro podocyte models is a clinical imperative. Our team studied human podocyte development using scRNA-seq technology and established a framework to unbiasedly evaluate human embryonic stem cells (hESC)-derived podocytes. Confocal imaging served as a powerful tool for us to capture compelling immunofluorescent and fluorescent in situ hybridization validations of transcriptomic findings. https://www.leica-microsystems.com//science-lab/multiphoton-imaging-bolsters-findings-on-in-vitro-differentiation-of-pluripotent-stem-cell-derived-podocyte/ Mon, 26 Aug 2019 11:38:00 +0000 Tracy Tran https://www.leica-microsystems.com/24680 Live-Cell Imaging Confluency Check with PAULA Cell Imager Many cell-based experiments require cells in a certain state. This can include their morphology,  fluorescent protein expression, and the confluency of the cell layer. Researchers check their cells regularly to determine these attributes. Confluency estimation can be very difficult! This guess work is biased by every individual researcher, and can result in lab error.  https://www.leica-microsystems.com//science-lab/confluency-check-with-paula-cell-imager/ Wed, 20 Feb 2019 23:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/20653 Laser Microdissection Webinar: Live Cell Isolation by Laser Microdissection Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections, laser microdissection may be applied for live cell isolation. Unlike other well-established and widely used techniques for live cell isolation and single cell cloning—such as FACS, MACS, cloning by limited dilution, and so on—laser microdissection allows for capturing live cells and cell colonies without their detachment from the carrier. https://www.leica-microsystems.com//science-lab/webinar-live-cell-isolation-by-laser-microdissection/ Tue, 16 Oct 2018 22:00:00 +0000 https://www.leica-microsystems.com/20037 CRISPR-Cas9 Role of circadian gene Clock during differentiation of mouse pluripotent stem cells Biological rhythms controlled by the circadian clock are absent in embryonic stem cells (ESCs). However, they start to develop during the differentiation of pluripotent ESCs to downstream cells. Conversely, biological rhythms in adult somatic cells disappear when they are reprogrammed into induced pluripotent stem cells (iPSCs). These studies indicated that the development of biological rhythms in ESCs might be closely associated with the maintenance and differentiation of ESCs. https://www.leica-microsystems.com//science-lab/role-of-circadian-gene-clock-during-differentiation-of-mouse-pluripotent-stem-cells/ Wed, 14 Mar 2018 23:00:00 +0000 https://www.leica-microsystems.com/19812 Widefield Microscopy Live-Cell Imaging Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia. https://www.leica-microsystems.com//science-lab/adipose-stem-cells-display-higher-regenerative-capacities-and-more-adaptable-electro-kinetic-properties-compared-to-bone-marrow-derived-mesenchymal-stromal-cells/ Sun, 21 Jan 2018 23:00:00 +0000 https://www.leica-microsystems.com/19451 Live-Cell Imaging Fluorescence Microscopy Multiphoton Microscopy Five Questions Asked: Prof. Dr. Jacco van Rheenen speaks about the most important considerations when imaging deep into mouse tissue When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to explain the significance of the pinhole for those, who did not want to spend too much time to dig into theory and details of confocal microscopy but wanted to have an idea about the effect of the pinhole. https://www.leica-microsystems.com//science-lab/five-questions-asked-prof-dr-jacco-van-rheenen-speaks-about-the-most-important-considerations-when-imaging-deep-into-mouse-tissue/ Fri, 19 May 2017 05:47:00 +0000 PhD Jacco van Rheenen, Dr. Bernd Sägmüller https://www.leica-microsystems.com/18730 Confocal Microscopy Live-Cell Imaging Adeno-associated Viral Vectors do not Efficiently Target Muscle Satellite Cells Adeno-associated viral (AAV) vectors are becoming an important tool for gene therapy of numerous genetic and other disorders. Several recombinant AAV vectors (rAAV) have the ability to transduce striated muscles in a variety of animals following intramuscular and intravascular administration, and have attracted widespread interest for therapy of muscle disorders such as the muscular dystrophies. Here we examined the relative ability of rAAV vectors derived from AAV6 to target myoblasts, myocytes, and myotubes in culture and satellite cells and myofibers in vivo. AAV vectors are able to transduce proliferating myoblasts in culture, albeit with reduced efficiency relative to postmitotic myocytes and myotubes. In contrast, quiescent satellite cells are refractory to transduction in adult mice. https://www.leica-microsystems.com//science-lab/adeno-associated-viral-vectors-do-not-efficiently-target-muscle-satellite-cells/ Mon, 05 Sep 2016 05:58:00 +0000 https://www.leica-microsystems.com/17977 Confocal Microscopy Live-Cell Imaging The Bimodally Expressed MicroRNA miR‐142 Gates Exit from Pluripotency A stem cell's decision to self‐renew or differentiate is thought to critically depend on signaling cues provided by its environment. It is unclear whether stem cells have the intrinsic capacity to control their responsiveness to environmental signals that can be fluctuating and noisy. Using a novel single‐cell microRNA activity reporter, we show that miR‐142 is bimodally expressed in embryonic stem cells, creating two states indistinguishable by pluripotency markers. https://www.leica-microsystems.com//science-lab/the-bimodally-expressed-microrna-mir-142-gates-exit-from-pluripotency/ Fri, 13 May 2016 13:47:00 +0000 https://www.leica-microsystems.com/16099 Confocal Microscopy "We can go home and the imaging is done automatically by the Leica HCS A Matrix Screener." Jutta Maria Bulkescher is the technical coordinator in the Novo Nordisk Foundation Center for Protein Research and Danish Stem Cell Center in Copenhagen, Denmark. The Leica HCS-A matrix screener is an invaluable tool for her facility. "It just gives us the biggest and easiest flexibility we can have to set up different imaging paramters and to check different conditions on one multi-well plate", explains Bulkescher. https://www.leica-microsystems.com//science-lab/we-can-go-home-and-the-imaging-is-done-automatically-by-the-leica-hcs-a-matrix-screener/ Wed, 19 Aug 2015 14:30:00 +0000 Jutta Bulkescher, PhD Isabelle Köster, Dipl. oec.-troph. Anja Schué https://www.leica-microsystems.com/15762 Confocal Microscopy Neuroscience Switching Roles: The Functional Plasticity of Adult Tissue Stem Cells Adult organisms have to adapt to survive, and the same is true for their tissues. Rates and types of cell production must be rapidly and reversibly adjusted to meet tissue demands in response to both local and systemic challenges. Recent work reveals how stem cell (SC) populations meet these requirements by switching between functional states tuned to homoeostasis or regeneration. https://www.leica-microsystems.com//science-lab/switching-roles-the-functional-plasticity-of-adult-tissue-stem-cells/ Mon, 18 May 2015 12:56:00 +0000 https://www.leica-microsystems.com/14774 Laser Microdissection Neuroscience Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and infiltrate brain parenchyma, they closely interact with a changing micro-environmental landscape that sustains tumor cell invasion. https://www.leica-microsystems.com//science-lab/identification-of-molecular-pathways-facilitating-glioma-cell-invasion-in-situ/ Mon, 22 Dec 2014 09:35:00 +0000 https://www.leica-microsystems.com/14066 Multiphoton Microscopy Live-Cell Imaging The Environment Makes the Stem Cell A recent publication in Nature shows that all stem cells divide and compete for niche space by passively "kicking out" others so that eventually one stem cell takes over the whole niche. Jacco van Rheenen and Saskia Ellenbroek talk about a new method of intravital imaging, which allows following the fate of individual stem cells over time in vivo and explains the new paradigm for stem cell development in the intestinal stem cell niche. https://www.leica-microsystems.com//science-lab/the-environment-makes-the-stem-cell/ Mon, 04 Aug 2014 16:25:00 +0000 PhD Jacco van Rheenen, PhD Saskia I. J. Ellenbroek, PhD Isabelle Köster https://www.leica-microsystems.com/10286 Live-Cell Imaging Multiphoton Microscopy Direct In Vivo Evidence for Tumor Propagation by Glioblastoma Cancer Stem Cells Cancer research using multiphoton microscopy. High-grade gliomas (World Health Organization grade III anaplastic astrocytoma and grade IV glioblastoma multiforme), the most prevalent primary malignant brain tumors, display a cellular hierarchy with self-renewing, tumorigenic cancer stem cells (CSCs) at the apex. While the CSC hypothesis has been an attractive model to describe many aspects of tumor behavior, it remains controversial due to unresolved issues including the use of ex vivo analyses with differential growth conditions. https://www.leica-microsystems.com//science-lab/direct-in-vivo-evidence-for-tumor-propagation-by-glioblastoma-cancer-stem-cells/ Fri, 12 Jul 2013 13:21:00 +0000 https://www.leica-microsystems.com/7275 Laser Microdissection Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome" The physiological process of aging is an elementary part of life. The processes responsible for the aging process and the effect they have is one of the key focuses of biomedical research. Dr. Yvonne Begus-Nahrmann researches molecular mechanisms in stem cells and their role in aging and tumor development at the Göttingen University Medical Center. Signal proteins such as p53 and p21 appear to play an important role in this context. To investigate these proteins in specific cell types, the cells are isolated from organ tissue with the aid of laser microdissection. https://www.leica-microsystems.com//science-lab/stem-cells-in-the-aging-process-the-signal-protein-p53-as-guardian-of-the-genome/ Thu, 08 Nov 2012 23:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille, Dr. Yvonne Begus-Nahrmann https://www.leica-microsystems.com/7290 Confocal Microscopy Live-Cell Imaging Nobel Prize 2012 in Physiology or Medicine for Stem Cell Research The Nobel Prize recognizes two scientists who discovered that mature, specialised cells can be reprogrammed to become immature cells capable of developing into all tissues of the body. Their findings have revolutionised our understanding of how cells and organisms develop. https://www.leica-microsystems.com//science-lab/nobel-prize-2012-in-physiology-or-medicine-for-stem-cell-research/ Tue, 09 Oct 2012 22:00:00 +0000 Nobel Media AB 2012 https://www.leica-microsystems.com/5929 Multiphoton Microscopy Confocal Microscopy Order versus Disorder In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular cross-talk between cells and scaffolds during tissue formation/remodeling. In bone substitutes chemical biomimesis has been particularly exploited; conversely, the relevance of pre-determined scaffold architecture for regenerated bone outputs is still unclear. https://www.leica-microsystems.com//science-lab/order-versus-disorder/ Thu, 19 Apr 2012 22:00:00 +0000 Paolo Giannoni, Paolo Bianco, Prof. Alberto Diaspro, Anna Tampieri, Ugo Valbusa, Giuseppe Firpo, Roberto Marotta, Monica Sandri, Paolo Bianchini, Rodolfo Quarto https://www.leica-microsystems.com/2561 Multiphoton Microscopy Live-Cell Imaging Confocal Microscopy Stem Cell Biology in Cancer Research The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence microscopy and confocal microscopy invaluable allies in the study of stem cells. https://www.leica-microsystems.com//science-lab/stem-cell-biology-in-cancer-research/ Thu, 22 Apr 2010 22:00:00 +0000 Laura Furia, Angelo Cicalese, Ilaria Rancati, Isabella Ponzanelli, Paolo Bianchini, Prof. Alberto Diaspro, Mario Faretta https://www.leica-microsystems.com/9447 Stereo Microscopy Detection and Characterization of Hepatic Engraftment of Embryonic Stem Derived Cells by Fluorescent Stereomicroscopy Embryonic stem (ES) cells have been investigated as a potential replacement therapy for failed organs, such as the liver. However, detection of hepatic engraftment from candidate stem cells has been difficult due to low engraftment efficiency. Previous detection methods required that the graft be processed by molecular and/or immunohistochemical techniques, limiting further functional studies. This study evaluated the use of three-dimensional fluorescent stereomicroscopy for gross detection of ES cell derived hepatic engraftment. https://www.leica-microsystems.com//science-lab/detection-and-characterization-of-hepatic-engraftment-of-embryonic-stem-derived-cells-by-fluorescent-stereomicroscopy/ Wed, 15 Aug 2007 13:23:00 +0000