Leica Science Lab - Tag : TCSPC https://www.leica-microsystems.com//science-lab/tag/tags/tcspc/show/Tag/ Article tagged with TCSPC en-US https://www.leica-microsystems.com/20109 Quantitative Fluorescence Lifetime – a Proper Alternative „Way too complicated!“ - the notorious feedback when it comes to fluorescence lifetime measurements. This will change now! New technologies and new concepts for data evaluation, all implemented in the new Leica SP8 FALCON, render fluorescence lifetime imaging (FLIM) as fuss-free as ordinary confocal imaging. And by the way: with Leica FALCON you can record frames 10 times faster compared to the classical standard. And three (or more) dimensional image stacks or time series are generated in a snap. Four channels simultaneously? No problem! And of course there are tunable excitation wavelength both visible with white light lasers (WLL) and infrared (the latter for multiphoton microscopy). That should be reason enough to delve into fluorescence lifetime imaging. The picture shows a lifetime image of a mouse embryo. Recorded in 722 stitched tiles and fitted for four separate characteristic times. Recording time ca 1 hour – compared to ca 1 day with the classical approach. https://www.leica-microsystems.com//science-lab/lifetime-a-proper-alternative/ Wed, 28 Mar 2018 22:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/3514 Confocal Microscopy Quantitative Fluorescence White Confocal Choose Your Excitation Wavelength Although time correlated single photon counting (TCSPC) is the method of choice for fluorescence lifetime quantification, it requires dedicated instrumentation including a pulsed laser source, a photon counting card, and a fast detector. https://www.leica-microsystems.com//science-lab/choose-your-excitation-wavelength/ Tue, 12 Apr 2011 22:00:00 +0000 Ph.D. Corentin Spriet, Ph.D. Aymeric Leray, Dave Trinel, Franck Riquet, Laurent Héliot