Leica Science Lab - Tag : THUNDER Imaging https://www.leica-microsystems.com//science-lab/tag/tags/thunder-imaging/show/Tag/ Article tagged with THUNDER Imaging en-US https://www.leica-microsystems.com/24935 Fluorescence Microscopy Live-Cell Imaging Widefield Microscopy Image Gallery: THUNDER Imager To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve this using Computational Clearing our new opto-digital technology. The result is high-speed, high-quality imaging of a large diversity of three-dimensional samples, including model organisms, tissue sections, and 3D cell cultures. Take a look at these images to see how THUNDER Imagers are already helping researchers to reveal the finest structural details even deep within a sample. Once you have seen the haze-free image quality achieved with THUNDER Imagers, find out more on our product page. Learn how THUNDER Imagers combine Computational Clearing with the speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes so you can decode 3D biology in real-time. https://www.leica-microsystems.com//science-lab/galleries/image-gallery-thunder-imager/ Tue, 12 May 2020 11:20:00 +0000 https://www.leica-microsystems.com/28338 Widefield Microscopy Computational Clearing - Enhance 3D Specimen Imaging This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related workflow. https://www.leica-microsystems.com//science-lab/computational-clearing-enhance-3d-specimen-imaging/ Fri, 08 May 2020 12:56:00 +0000 https://www.leica-microsystems.com/27800 Widefield Microscopy THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue sections to monitoring calcium waves in Arabidopsis seedlings and much more. https://www.leica-microsystems.com//science-lab/thunder-imagers-high-performance-versatility-and-ease-of-use-for-your-everyday-imaging-workflows/ Wed, 25 Mar 2020 12:56:00 +0000 https://www.leica-microsystems.com/28066 Widefield Microscopy Evaluating Axon Regeneration After Brain or Spine Trauma of Mice Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and non-active axons using widefield and THUNDER imaging technology. Results indicated a better discrimination between active and non-active axons in THUNDER images. https://www.leica-microsystems.com//science-lab/evaluating-axon-regeneration-after-brain-or-spine-trauma-of-mice/ Tue, 17 Mar 2020 11:55:00 +0000 PhD James DeRose, Robert Fasulka https://www.leica-microsystems.com/27942 Fluorescence Microscopy Widefield Microscopy Interview with Magali Mondin on THUNDER Imagers Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had the opportunity to test extensively the THUNDER Imager 3D Tissue in her lab for several weeks on a wide variety of specimens. We met with her after the testing was finished to discuss the results she obtained and her experience working with the THUNDER technology. The BIC offers resources for photonic and electronic imaging, mainly in the life, health, and plant sciences. It is a core facility in France with a staff of 12 highly skilled engineers. The BIC has advanced bio-imaging techniques for fixed- and live-cell imaging, such as video microscopy, confocal microscopy, multiphoton microscopy, transmission electron microscopy, and scanning electron microscopy. https://www.leica-microsystems.com//science-lab/interview-with-magali-mondin-on-thunder-imagers/ Mon, 09 Mar 2020 12:43:00 +0000 Engineer Magali Mondin https://www.leica-microsystems.com/26925 Widefield Microscopy Drosophila Testis Niche Stem Cells – Three Color Computational Clearing Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only partially) fixed. Such cells are called stem cells. Stem cells serve to proliferate new cells for the specific tasks, but at the same time always produce stem cells, which in turn can supply new specific cells when needed later. https://www.leica-microsystems.com//science-lab/drosophila-testis-niche-stem-cells-three-color-computational-clearing/ Mon, 04 Nov 2019 11:42:00 +0000 Linda B. Morgan, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/25096 Widefield Microscopy Alzheimer Plaques: fast Visualization in Thick Sections More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this disease. A few treatments try to slow down the fatal path or try to relieve the patients from symptoms. The laboratory of Dr. Mehrdad Shamloo at Stanford University studies pathological brain function with the goal of contributing to the discovery of novel therapeutics for Alzheimer’s disease. They use a mouse model of this disease to study the role of inflammation in Alzheimer’s disease progression. This requires imaging of thick uncleared brain tissues. https://www.leica-microsystems.com//science-lab/alzheimer-plaques-fast-visualization-in-thick-sections/ Thu, 27 Jun 2019 10:50:00 +0000 PhD Olga Davydenko, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24637 Widefield Microscopy Fluorescence Microscopy Live-Cell Imaging Stereo Microscopy THUNDER Technology Note So far, widefield microscopy was not suitable to image larger volumes, since the contrast of the recorded fluorescence image is reduced by the background (BG) mainly originating from out of focus regions of the recorded volume. BG reduces the effective dynamic range, the maximal possible signal to noise ratio (SNR), of the imaging system. https://www.leica-microsystems.com//science-lab/thunder-technology-note/ Tue, 05 Mar 2019 23:00:00 +0000 Dr. Jan Schumacher, Louise Bertrand