Leica Science Lab - Tag : Tissue Clearing https://www.leica-microsystems.com//science-lab/tag/tags/tissue-clearing/show/Tag/ Article tagged with Tissue Clearing en-US https://www.leica-microsystems.com/25096 Widefield Microscopy Alzheimer Plaques: fast Visualization in Thick Sections More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this disease. A few treatments try to slow down the fatal path or try to relieve the patients from symptoms. The laboratory of Dr. Mehrdad Shamloo at Stanford University studies pathological brain function with the goal of contributing to the discovery of novel therapeutics for Alzheimer’s disease. They use a mouse model of this disease to study the role of inflammation in Alzheimer’s disease progression. This requires imaging of thick uncleared brain tissues. https://www.leica-microsystems.com//science-lab/alzheimer-plaques-fast-visualization-in-thick-sections/ Thu, 27 Jun 2019 10:50:00 +0000 PhD Olga Davydenko, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24781 Confocal Microscopy Neuroscience Zebrafish Brain - Whole Organ Imaging at High Resolution Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete brain dissected from a developing zebrafish, one would need to cover a field of some ten square millimeters at a depth in the millimeter range. Usually, low magnification lenses do not provide sufficient resolution to reveal the intricate structural interactions in nervous tissue. Additionally, due to scattering processes, the depth at which one can image within dense biological tissue using a confocal microscope is generally restricted to approximately 10 microns. https://www.leica-microsystems.com//science-lab/zebrafish-brain-whole-organ-imaging-at-high-resolution/ Mon, 08 Apr 2019 22:00:00 +0000 Ryan E. Robinson, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/20142 Multiphoton Microscopy Confocal Microscopy High-Resolution 3D Imaging of Whole Organ after Clearing Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have focused on 2D images, which is time consuming and implies extrapolation of results. Three-dimensional imaging of whole organs recently became an important challenge to better understand their architecture and allow cell enumeration. https://www.leica-microsystems.com//science-lab/high-resolution-3d-imaging-of-whole-organ-after-clearing/ Tue, 08 May 2018 22:00:00 +0000 https://www.leica-microsystems.com/19111 Confocal Microscopy Light Sheet Microscopy Multiphoton Microscopy Neuroscience Image Restoration and Deconvolution Clarifying Tissue Clearing Biological specimens are intrinsically three dimensional; however because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the scatter by “clearing” the tissue have been ongoing for over a century, there have been a large number of recent innovations. This review introduces the physical basis for light-scatter in tissue, describes the mechanisms underlying various clearing techniques, and discusses several of the major advances in light microscopy for imaging cleared tissue. https://www.leica-microsystems.com//science-lab/clarifying-tissue-clearing/ Thu, 26 Jan 2017 14:39:00 +0000 PhD Douglas Richardson, Dr. Jeff Lichtman https://www.leica-microsystems.com/18779 Multiphoton Microscopy BABB Clearing and Imaging for High Resolution Confocal Microscopy: Counting and Sizing Kidney Cells in the 21st Century Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective. Understanding kidney microanatomy is key to detecting and identifying early events in kidney disease. Improvements in tissue clearing and imaging have been crucial in this field, and now we report on a novel, time-efficient method to study podocyte depletion in renal glomeruli using a combination of immunofluorescence, optical clearing, confocal microscopy and 3D analysis. https://www.leica-microsystems.com//science-lab/babb-clearing-and-imaging-for-high-resolution-confocal-microscopy-counting-and-sizing-kidney-cells-in-the-21st-century/ Tue, 13 Sep 2016 09:03:00 +0000 Ph.D. Victor G. Puelles, Prof. John F. Bertram, MSc Stephen Firth, Prof. Ian Harper https://www.leica-microsystems.com/18796 Multiphoton Microscopy Confocal Microscopy Light Sheet Microscopy Neuroscience Clearing of Fixed Tissue: A Review from a Microscopist’s Perspective Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing interest in this field has both triggered and been stimulated by recent advances in high-throughput microscopy and data analysis methods, which allowed imaging and management of large samples. https://www.leica-microsystems.com//science-lab/clearing-of-fixed-tissue-a-review-from-a-microscopists-perspective/ Mon, 12 Sep 2016 11:20:00 +0000 https://www.leica-microsystems.com/17957 Super-Resolution Confocal Microscopy Light Sheet Microscopy Neuroscience Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for high-resolution fluorescence imaging (SeeDB2). SeeDB2 matches the refractive indices of fixed tissues to that of immersion oil (1.518), thus minimizing both light scattering and spherical aberrations. https://www.leica-microsystems.com//science-lab/super-resolution-mapping-of-neuronal-circuitry-with-an-index-optimized-clearing-agent/ Wed, 27 Apr 2016 10:07:00 +0000 https://www.leica-microsystems.com/17507 Super-Resolution How to Combine STED and CLARITY Previously, the preferred way to study the subtlest elements of the kidney, such as foot processes and the slit diaphragm has been by the use of electron microscopy. Using STED microscopy, we show that the nanoscale localization of slit diaphragm proteins can now be resolved using light microscopy. Even if the nanoscopic resolution has been available for a decade, light microscopy studies of the slit diaphragm are not found in the literature. This is likely due to the difficulties of achieving the high quality of fluorescent labelling needed for super-resolution microscopy. By applying an optical clearing protocol based on the CLARITY technique, we found that the immunostaining quality in kidney tissue can be improved. The improvement is likely due to the removal of lipids, resulting in a higher availability of binding epitopes in cleared tissue, as compared to PFA fixed non-cleared tissue. https://www.leica-microsystems.com//science-lab/how-to-combine-sted-and-clarity/ Fri, 29 Jan 2016 14:46:00 +0000 https://www.leica-microsystems.com/14727 Confocal Microscopy Multiphoton Microscopy Deeper Insights in Transparent Animals CLARITY clearing derivatives for multiphoton microscopy. Transparent organisms help us to identify spatial arrangements and connections of cells and tissues, especially neuronal circuits can easily be identified and characterized. CLARITY is on everyone's lips. https://www.leica-microsystems.com//science-lab/deeper-insights-in-transparent-animals/ Mon, 10 Nov 2014 09:48:00 +0000 BS, PhD Viviana Gradinaru, PhD Isabelle Köster https://www.leica-microsystems.com/14215 Multiphoton Microscopy Neuroscience Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing Understanding the structure-function relationships at cellular, circuit, and organ-wide scale requires 3D anatomical and phenotypical maps, currently unavailable for many organs across species. At the root of this knowledge gap is the absence of a method that enables whole-organ imaging. Herein, we present techniques for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically transparent, thereby exposing their cellular structure with intact connectivity. https://www.leica-microsystems.com//science-lab/single-cell-phenotyping-within-transparent-intact-tissue-through-whole-body-clearing/ Wed, 20 Aug 2014 11:26:00 +0000