Leica Science Lab - Tag : Tissue Regeneration https://www.leica-microsystems.com//science-lab/tag/tags/tissue-regeneration/show/Tag/ Article tagged with Tissue Regeneration en-US https://www.leica-microsystems.com/18730 Confocal Microscopy Live-Cell Imaging Adeno-associated Viral Vectors do not Efficiently Target Muscle Satellite Cells Adeno-associated viral (AAV) vectors are becoming an important tool for gene therapy of numerous genetic and other disorders. Several recombinant AAV vectors (rAAV) have the ability to transduce striated muscles in a variety of animals following intramuscular and intravascular administration, and have attracted widespread interest for therapy of muscle disorders such as the muscular dystrophies. Here we examined the relative ability of rAAV vectors derived from AAV6 to target myoblasts, myocytes, and myotubes in culture and satellite cells and myofibers in vivo. AAV vectors are able to transduce proliferating myoblasts in culture, albeit with reduced efficiency relative to postmitotic myocytes and myotubes. In contrast, quiescent satellite cells are refractory to transduction in adult mice. https://www.leica-microsystems.com//science-lab/adeno-associated-viral-vectors-do-not-efficiently-target-muscle-satellite-cells/ Mon, 05 Sep 2016 05:58:00 +0000 https://www.leica-microsystems.com/15762 Confocal Microscopy Neuroscience Switching Roles: The Functional Plasticity of Adult Tissue Stem Cells Adult organisms have to adapt to survive, and the same is true for their tissues. Rates and types of cell production must be rapidly and reversibly adjusted to meet tissue demands in response to both local and systemic challenges. Recent work reveals how stem cell (SC) populations meet these requirements by switching between functional states tuned to homoeostasis or regeneration. https://www.leica-microsystems.com//science-lab/switching-roles-the-functional-plasticity-of-adult-tissue-stem-cells/ Mon, 18 May 2015 12:56:00 +0000 https://www.leica-microsystems.com/13257 Multiphoton Microscopy Live-Cell Imaging Tracking Glomerular Fate Over Long Time Distances Multi-channel multiphoton microscopy with dedicated optics for CLARITY. The glomerular filtration barrier (GFB) is a complex spatial structure within the kidney glomerulis where ultrafiltration takes place. Podocytes are critical elements of the GFB and take part in the filtration process. They have been shown to be involved in the development of kidney diseases. However, due to technical limitations, the mechanism of glomerular pathology is not well understood. https://www.leica-microsystems.com//science-lab/tracking-glomerular-fate-over-long-time-distances/ Mon, 14 Apr 2014 12:32:00 +0000 M.D., Ph.D. Janos Peti-Peterdi, PhD Isabelle Köster https://www.leica-microsystems.com/10106 Confocal Microscopy Stereo Microscopy Fluorescence Microscopy Organ Regeneration: An Unlikely Fish Tale Spectacular discoveries in cardiac tissue regeneration are rapidly moving researchers closer to the goal of harnessing regenerative techniques to repair the human heart. Only eleven years ago, Dr. Kenneth Poss, Professor of Cell Biology at Duke University and an Early Career Scientist of the Howard Hughes Medical Institute, published the first research to clearly visualize an example of cardiac tissue regeneration using fluorescence microscopy. https://www.leica-microsystems.com//science-lab/organ-regeneration-an-unlikely-fish-tale/ Thu, 20 Jun 2013 08:01:00 +0000 PhD Katharine H. Hendrix